Restorative Effect of Distillated Nerium Oleander Extract on Diabetic Neuropathy: Animal Model Study

Abstract

The current study aims to investigate the possible role of Nerium oleander (NeOL) distillate in the treatment of diabetic neuropathy. The restorative affects of distillated NeOL on the diabetes-induced electrophysiological alterations were investigated. Induction of diabetes was done by the combination of single dose streptozotocin injection together with a high fat diet for four weeks. Experimental groups were designed as follows: control, diabetic, and NeOL treated diabetic. Nerve conduction velocity (CV) recordings were performed through a suction electrode from the tibia branch of the sciatic nerve trunk. Diabetes results an increase in rheobase, chronaxie values together with a decrease in maximum depolarization (MD), compound action potential (CAP) area and CV measurements. With its antioxidant nature, NeOL treatment produced nearly complete restorations of the diabetes-induced alterations. Current study has shown that distillated NeOL (375 µg /0.5 ml dH2O /day) can be a highly potential therapeutic agent on the diabetes induced alterations.

Effects of the Experiment Conditions on the Nerve Action Potential: A Model Study

“…The other problem on this issue is that whether the experimental models reflect the clinical situation or not? At this point age, gender, body weight, species, body temperature of the experimental animals and the environmental factors must be taken into account [4][5][6].…”