Restraint stress induces uterine microenvironment disorder in mice during early pregnancy through the β<sub>2</sub>-AR/cAMP/PKA pathway

Lu, Jun; Liu, Guanhui; Wang, Zixu; Cao, Jing; Chen, Yaoxing; Dong, Yulan

doi:10.1080/10253890.2020.1855419

Cited by 6 publications

(1 citation statement)

References 90 publications

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“…The SCN5A gene is a myocardial cell Na + channel that regulates myocardial cell action potential and is involved in the occurrence and development of cardiomyopathy [6]. Genes such as AR [14] and PGR [15] are involved in hormone regulation in the body, thereby improving heart failure symptoms [16], and reducing mortality [17]. Seven core targets, including CASP3, CXCL8 (IL-8), IL6, MAPK1, TNF, TP53, and VEGFA, were nally derived by constructing a PPI network analysis and were considered effective targets in the treatment of PS.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Wu,

Guo,

Zhang

et al. 2023

Preprint

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Purpose: NvZhen ErXian HeJi (NZEXHJ) is used to treat perimenopausal syndrome (PS), but its effect on perimenopausal coronary heart disease is unclear. To study the effect of NZEXHJ on perimenopausal coronary heart disease in a rat model based on a network pharmacology approach. Methods: Based on network pharmacological analysis combined with molecular docking, and that predicted the potential therapeutic target and pharmacological mechanism of NZEXHJ in the treatment of PMCHD. We used an ovariectomized rat (OVR) model to understand the effect of NZEXHJ on myocardial injury and further verify the target of NZEXHJ in the intervention of PMCHD. Results: We selected 52 active components of NZEXHJ against PMCHD, and an intersection of their targets on network pharmacology, which SCN5A, SER1, AR, and PGR were significantly correlated. Protein-protein interaction network revealed CASP3, CXCL8, IL6, MAPK1, TNF, TP53, and VEGFA, in the treatment of PMCHD with NZEXHJ. The Kaempferol, luteolin, and mistletoe pre-sented good affinity towards the aforementioned targets by Molecular docking NZEXHJ exerted protecting cardiomyocytes for OVR. The mechanism was related to a reduction in the expression levels of the CXCL8, TNF, and regulate PI3K-AKT signaling pathways. Conclusion: NZEXHJ may protect against myocardial injury after myocardial infarction in ovariectomized rats by regulating the PI3K-AKT signaling pathway through CXCL8, TNF, and other targets. Our study provides new ideas and targets for the treatment of perimenopausal coronary heart disease in the future.

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Section: Discussionmentioning

confidence: 99%

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Wu,

Guo,

Zhang

et al. 2023

Preprint

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Integrating pharmacokinetics and network pharmacology to identify and validate targets of Guben Xiaozhen prescription for the treatment of chronic urticaria

Ren

Liu

et al. 2022

Journal of Ethnopharmacology

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NE-activated β₂-AR/β-arrestin 2/Src pathway mediates duodenal hyperpermeability induced by water-immersion restraint stress

Yan

Zhu

Liang

et al. 2023

American Journal of Physiology-Cell Physiology

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Stress causes a rapid spike in norepinephrine (NE) levels, leading to gastrointestinal dysfunction. NE reduces the expression of tight junctions (TJs) and aggravates intestinal mucosal damage, but the regulatory mechanism is still unclear. The present study aimed to investigate the molecular mechanisms underlying the regulation of stress-associated duodenal hyperpermeability by NE. Fluorescein isothiocyanate -dextran permeability, transepithelial resistance, immunofluorescence, Western blot and high-performance liquid chromatography analysis were used in water-immersion restraint stress (WIRS) rats in this study. The results indicate that the duodenal permeability, degradation of TJs, mucosal NE and β2-adrenergic receptor (β2-AR), increased in WIRS rats. The duodenal intracellular cyclic adenosine monophosphate levels were decreased, while the expression of β-arrestin 2, negatively regulates G-protein coupled receptors signaling, was significantly increased. Src recruitment was mediated by β-arrestin; thus, the levels of Src kinase activation were enhanced in WIRS rats. NE depletion, β2-AR, or β-arrestin 2 blockade significantly decreased mucosal permeability and increased TJs expression, suggesting improved mucosal barrier function. Moreover, NE induced an increased duodenal permeability of normal rats with activated β-arrestin 2/Src signaling, which was significantly inhibited by β2-AR blockade. The present findings demonstrate that the enhanced NE induced an increased duodenal permeability in WIRS rats through the activated β2-AR/β-arrestin 2/Src pathway. This study provides novel insight into the molecular mechanism underlying the regulation of NE on the duodenal mucosal barrier and a new target for treating duodenal ulcers induced by stress.

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Restraint stress induces uterine microenvironment disorder in mice during early pregnancy through the β₂-AR/cAMP/PKA pathway

Cited by 6 publications

References 90 publications

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Integrating pharmacokinetics and network pharmacology to identify and validate targets of Guben Xiaozhen prescription for the treatment of chronic urticaria

NE-activated β₂-AR/β-arrestin 2/Src pathway mediates duodenal hyperpermeability induced by water-immersion restraint stress

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Restraint stress induces uterine microenvironment disorder in mice during early pregnancy through the β2-AR/cAMP/PKA pathway

Cited by 6 publications

References 90 publications

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Mechanism of action of NvZhen ErXian HeJi in ovariectomized rats with myocardial infarction based on network pharmacology

Integrating pharmacokinetics and network pharmacology to identify and validate targets of Guben Xiaozhen prescription for the treatment of chronic urticaria

NE-activated β2-AR/β-arrestin 2/Src pathway mediates duodenal hyperpermeability induced by water-immersion restraint stress

Contact Info

Product

Resources

About

Restraint stress induces uterine microenvironment disorder in mice during early pregnancy through the β₂-AR/cAMP/PKA pathway

NE-activated β₂-AR/β-arrestin 2/Src pathway mediates duodenal hyperpermeability induced by water-immersion restraint stress