1993
DOI: 10.1002/aja.1001980405
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Restricted expression of Mov13 mutant α1(I) collagen gene in osteoblasts and its consequences for bone development

Abstract: Cell type-specific differences in the transcriptional control of the mouse gene coding for the a1 chain of collagen type I (Collal) have been revealed previously with the help of the Movl3 mouse strain which carries a retroviral insert in the first intron of the gene. Transcription of this mutant Collal allele is completely blocked in all mesodermal cell types tested so far, with the exception of the odontoblast where it is expressed at an apparently normal rate (Kratochwil et al. [1989] Cell 5E807-816). To de… Show more

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Cited by 19 publications
(14 citation statements)
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“…Subsequent studies showed that the insertional mutation inactivated Col1a1 in most cells, with the exception of odontoblasts and osteoblasts. 18,19 These initial reports sparked a number of sometimes conflicting studies from laboratories that attempted to define the sequences that were inactivated by this mutation. 5 Eventually, the preponderance of evidence supported the existence of orientation-dependent, cell-specific transcriptional elements in the first intron of the ␣1(I) collagen gene in both mice and humans.…”
Section: Discussionmentioning
confidence: 99%
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“…Subsequent studies showed that the insertional mutation inactivated Col1a1 in most cells, with the exception of odontoblasts and osteoblasts. 18,19 These initial reports sparked a number of sometimes conflicting studies from laboratories that attempted to define the sequences that were inactivated by this mutation. 5 Eventually, the preponderance of evidence supported the existence of orientation-dependent, cell-specific transcriptional elements in the first intron of the ␣1(I) collagen gene in both mice and humans.…”
Section: Discussionmentioning
confidence: 99%
“…However, patients occasionally develop dissection of the aorta 16 Although a targeted disruption of the Col1a1 gene has not been performed, the random insertion of the Molony murine leukemia virus into the first intron of Col1a1 inactivated the gene in most tissues, including blood vessels. 18,19 These mice die at day 11 to 14 of embryonic age from rupture of blood vessels. The availability of ⌬/⌬ mice, in which an agedependent reduction in collagen synthesis in a number of tissues had been demonstrated, has made it possible to study the function of the first intron in a more systematic manner.…”
mentioning
confidence: 99%
“…Nonspecific processing of the C-propeptide of type I procollagen is occasionally observed in tissue culture (29,30) but not in vivo (13,14). No genetic defects in the enzyme have been detected in surveys of hundreds of patients with genetic diseases of connective tissues (14), and transgenic mice without fibrils of type I collagen (31,32) …”
Section: Discussionmentioning
confidence: 99%
“…Subsequently, it was demonstrated that the retroviral insertion led to transcriptional inactivation of the gene in mouse embryo cell lines (24). It was also shown that the Col1A1 gene was transcribed in odontoblasts and osteoblasts and that in these cells, the first intron along with the integrated retrovirus was spliced out (30,31,48). These findings implied that incorrect splicing could not account for the lethality of the mutation in homozygous mice and that different cis-acting elements function in the regulation of Col1A1 in different cells.…”
mentioning
confidence: 99%