Restricted expression of mutant SOD1 in spinal motor neurons and interneurons induces motor neuron pathology

Wang, Lijun; Sharma, Kamal; Deng, Han Xiang; Siddique, Teepu; Grisotti, Gabriella; Liu, Erdong

doi:10.1016/j.nbd.2007.10.004

Cited by 91 publications

(91 citation statements)

References 18 publications

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“…The ROSA:LNL:tTA system effectively induced Notch4* expression, resulting in even higher protein levels than Tie2-tTAdriven expression (SI Appendix, Fig. S13 and Movie S3) (25). As demonstrated in other tissues (26), BMX(PAC)-CreERT2 efficiently activated the same reporter throughout the arterial trunk Fig.…”

Section: Ec Area But Not Number or Proliferation Increased In Av Shuntmentioning

confidence: 78%

Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels

Murphy

Kim

Huang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Brain arteriovenous malformations are focal lesions of enlarged, tangled vessels that shunt blood from arteries directly to veins. They can cause ischemia, hemorrhage, disability, and death, particularly in young people, accounting for 50% of childhood stroke. The molecular etiology of the disease remains poorly understood, hindering the development of therapeutic treatments. Here, we report that, in an animal model, the lesion arises from the enlargement of capillary-like vessels. Notch signaling in the endothelium of microvasculature and veins is critical for the disease initiation by increasing cell areas but not proliferation. Blood flow mediates disease progression by a positive feedback of increasing flow and vessel diameter. Our data shed light on the mechanism underlying the pathogenesis of this devastating disease.

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Section: Ec Area But Not Number or Proliferation Increased In Av Shuntmentioning

confidence: 78%

Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels

Murphy

Kim

Huang

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Corin-cre (Enshell-Seijffers et al, 2010a), ROSA:LNL: rtTA-IRES-EGFP (Belteki et al, 2005), ROSA:LNL:tTA (Wang et al, 2008), tetODTA/+ (Lee et al, 1998) and rYFP (Srinivas et al, 2001). ) to prevent melanin from obscuring cells in the upper DP.…”

Section: Micementioning

confidence: 99%

“…Corin-cre was used to initiate expression of a credependent tet transactivator allele (r26 Lee et al, 1998;Wang et al, 2008) (Fig. 3A).…”

Section: Dp Cell Number Specifies Hair Size and Shapementioning

confidence: 99%

Dermal papilla cell number specifies hair size, shape and cycling and its reduction causes follicular decline

2013

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Although the hair shaft is derived from the progeny of keratinocyte stem cells in the follicular epithelium, the growth and differentiation of follicular keratinocytes is guided by a specialized mesenchymal population, the dermal papilla (DP), that is embedded in the hair bulb. Here we show that the number of DP cells in the follicle correlates with the size and shape of the hair produced in the mouse pelage. The same stem cell pool gives rise to hairs of different sizes or types in successive hair cycles, and this shift is accompanied by a corresponding change in DP cell number. Using a mouse model that allows selective ablation of DP cells in vivo, we show that DP cell number dictates the size and shape of the hair. Furthermore, we confirm the hypothesis that the DP plays a crucial role in activating stem cells to initiate the formation of a new hair shaft. When DP cell number falls below a critical threshold, hair follicles with a normal keratinocyte compartment fail to generate new hairs. However, neighbouring follicles with a few more DP cells can re-enter the growth phase, and those that do exploit an intrinsic mechanism to restore both DP cell number and normal hair growth. These results demonstrate that the mesenchymal niche directs stem and progenitor cell behaviour to initiate regeneration and specify hair morphology. Degeneration of the DP population in mice leads to the types of hair thinning and loss observed during human aging, and the results reported here suggest novel approaches to reversing hair loss.

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“…SSTFlp;nNOS-CreER mice were first bred with a Rosa26-loxPNeoSTOPloxP-tTA (LNL-tTA) (Wang et al, 2008) line to generate SST-Flp;nNOS-CreER;LNL-tTA mice, in which tamoxifen induction can convert CreER into constitutive tTA expression in nNOS neurons. We further generated a new AAV vector in which TVA-mcherry expression is dependent on both tTA and Flp activities ( Figure 1D, Table 1, Figure 5I).…”

Section: Combinatorial Sst Nnos and Viral Targeting Captures Corticamentioning

confidence: 99%

Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex

He¹,

Tucciarone²,

Lee³

et al. 2016

Neuron

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SummarySystematic genetic access to GABAergic cell types will facilitate studying the function and development of inhibitory circuitry. However, single gene-driven recombinase lines mark relatively broad and heterogeneous cell populations. Although intersectional approaches improve precision, it remains unclear whether they can capture cell types defined by multiple features. Here we demonstrate that combinatorial genetic and viral approaches target restricted GABAergic subpopulations and cell types characterized by distinct laminar location, morphology, axonal projection, and electrophysiological properties. Intersectional embryonic transcription factor drivers allow finer fate mapping of progenitor pools that give rise to distinct GABAergic populations, including laminar cohorts. Conversion of progenitor fate restriction signals to constitutive recombinase expression enables viral targeting of cell types based on their lineage and birth time. Properly designed intersection, subtraction, conversion, and multi-color reporters enhance the precision and versatility of drivers and viral vectors. These strategies and tools will facilitate studying GABAergic neurons throughout the mouse brain. eTOC BlurbHe et al. present strategies and tools for the combinatorial targeting of GABAergic neurons in the mouse brain. Specific cell type targeting can be achieved by combining 2 to 3 driver-reporter alleles with viral vectors that engage multiple cell-defining features.

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Restricted expression of mutant SOD1 in spinal motor neurons and interneurons induces motor neuron pathology

Cited by 91 publications

References 18 publications

Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels

Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels

Dermal papilla cell number specifies hair size, shape and cycling and its reduction causes follicular decline

Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex

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