Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1.

Grail, Ann; Harbour, D. A.

doi:10.1292/jvms1939.52.1007

Cited by 10 publications

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“…This is similar to the situation for BHV-6 [9]. However, from examination of 50 field isolates we have shown that the FHV-1 genome is not unique and that variants occur sporadically [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionsupporting

confidence: 58%

Restriction endonuclease mapping of the genome of feline herpesvirus type 1

Grail

Harbour

Chia

1991

Archives of Virology

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Restriction endonuclease mapping was used to determine the molecular structure of feline herpesvirus type 1 (FHV-1) strain B 927. FHV-1 DNA purified from infected cells by pulsed field gel electrophoresis was partially restricted with Sau 3A to generate a series of overlapping fragments of 10-20 kb. EMBL 4 DNA was digested with Bam HI and Sal I and viral DNA was inserted into the "stuffer" region. Recombinant phage were selected on E. coli strain Q359. Single and double digestions with the enzymes Eco RI, Bam HI, Sal I, Sst I and Xho I enabled construction of physical maps of the genome. The FHV-1 genome is approximately 126 kbp in length and is composed of a long unique region of 99 kbp and a short region of 27 kbp comprising an unique sequence of 8-9 kbp flanked by inverted repeats of 7-8.5 kbp.

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Section: Introductionsupporting

confidence: 58%

Restriction endonuclease mapping of the genome of feline herpesvirus type 1

Grail

Harbour

Chia

1991

Archives of Virology

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“…Most produce a relatively uniform disease although some show reduced or increased virulence [39,42]. Antigenically all isolates belong to one serotype, and they are relatively homogeneous on restriction enzyme analysis of their DNA [41,52]. Some minor genetic differences have been reported for some strains, including differences in Mlu 1 cleavage patterns, re-arrangements in the gC gene, and differences in the Sal 1 site in the UL 17 herpes simplex virus gene homologue [43,44,59,76].…”

Section: Aetiologymentioning

confidence: 99%

Feline herpesvirus

et al. 2007

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“…Early studies demonstrated that isolates of FHV-1 were antigenically indistinguishable using conventional serological techniques. Additionally, a high level of genomic homogeneity was identified using restriction endonuclease cleavage analyses [10,11]. A subsequent restriction endonuclease cleavage analysis study of 78 Japanese isolates detected three FHV-1 genotypes, although these genotypes still appeared to be very similar [12].…”

Section: Introductionmentioning

confidence: 99%

Low genetic diversity among historical and contemporary clinical isolates of felid herpesvirus 1

et al. 2016

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Background: Felid herpesvirus 1 (FHV-1) causes upper respiratory tract diseases in cats worldwide, including nasal and ocular discharge, conjunctivitis and oral ulceration. The nature and severity of disease can vary between clinical cases. Genetic determinants of virulence are likely to contribute to differences in the in vivo phenotype of FHV-1 isolates, but to date there have been limited studies investigating FHV-1 genetic diversity. This study used next generation sequencing to compare the genomes of contemporary Australian clinical isolates of FHV-1, vaccine isolates and historical clinical isolates, including isolates that predated the introduction of live attenuated vaccines into Australia. Analysis of the genome sequences aimed to assess the level of genetic diversity, identify potential genetic markers that could influence the in vivo phenotype of the isolates and examine the sequences for evidence of recombination. Results: The full genome sequences of 26 isolates of FHV-1 were determined, including two vaccine isolates and 24 clinical isolates that were collected over a period of approximately 40 years. Analysis of the genome sequences revealed a remarkably low level of diversity (0.0-0.01 %) between the isolates. No potential genetic determinants of virulence were identified, but unique single nucleotide polymorphisms (SNPs) in the UL28 and UL44 genes were detected in the vaccine isolates that were not present in the clinical isolates. No evidence of FHV-1 recombination was detected using multiple methods of recombination detection, even though many of the isolates originated from cats housed in a shelter environment where high infective pressures were likely to exist. Evidence of displacement of dominant FHV-1 isolates with other (genetically distinct) FHV-1 isolates over time was observed amongst the isolates obtained from the shelter-housed animals. Conclusions:The results show that FHV-1 genomes are highly conserved. The lack of recombination detected in the FHV-1 genomes suggests that the risk of attenuated vaccines recombining to generate virulent field viruses is lower than has been suggested for some other herpesviruses. The SNPs detected only in the vaccine isolates offer the potential to develop PCR-based methods of differentiating vaccine and clinical isolates of FHV-1 in order to facilitate future epidemiological studies.

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Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1.

Cited by 10 publications

References 0 publications

Restriction endonuclease mapping of the genome of feline herpesvirus type 1

Restriction endonuclease mapping of the genome of feline herpesvirus type 1

Feline herpesvirus

Low genetic diversity among historical and contemporary clinical isolates of felid herpesvirus 1

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