Restriction endonuclease analysis of Leptospira interrogans serovar hardjo isolates from cattle

Ellis, William; Thiermann, A B; Montgomery, J.M.; Handsaker, A L; Winter, P.J.; Marshall, R.B.

doi:10.1016/s0034-5288(18)30874-9

Cited by 49 publications

(31 citation statements)

References 17 publications

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“…Due to their speed and sensitivity, several molecular techniques including the use of serovar-specific probes in DNA hybridization experiments (Yasuda et al, 1987;Terpstra et al, 1986;Zuener & Bolin, 1990), RFLP analysis (Marshall et al, 1984;Ellis et al, 1988) and ribotyping of 16S rRNA (Merien et al, 1992;Ralph et al, 1993;Perolat et al, 1994) have been used in phylogenetic studies and in the identification of strains and serovars of Leptospira. Although they are very useful for the genetic identification of serovars of Leptospira, many of these techniques present limitations such as being restricted to specific radioactively labelled probes, in addition to the use of complex technical procedures.…”

Section: Discussionmentioning

confidence: 99%

Low-stringency single specific primer PCR for identification of Leptospira

Oliveira¹,

Caballero²,

Vágó³

et al. 2003

Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 99%

Low-stringency single specific primer PCR for identification of Leptospira

Oliveira¹,

Caballero²,

Vágó³

et al. 2003

Journal of Medical Microbiology

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“…The most prevalent subtypes in cattle herds are Leptospira borgpetersenii serovar Hardjo and Leptospira interrogans serovar Hardjo. Differences among these subtypes are related to the geographic areas of isolation, genomic patterns and pathogenic features [5]. Hardjoprajitno isolates have been described in Australia, the United States of America (USA), the United Kingdom, Mexico, and Canada [6].…”

Section: Introductionmentioning

confidence: 99%

Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock

et al. 2017

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BackgroundLeptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008.ResultsSerological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains.ConclusionThis study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1081-9) contains supplementary material, which is available to authorized users.

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“…Clinical signs of hardjo infection include abortion and milk drop syndrome. Whilst there are genetic, epidemiological and pathogenic differences between the two species, the two microorganisms are indistinguishable by serological tests [17,28]. …”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibodies to Leptospira interrogans serovar hardjoin bulk tank milk from unvaccinated irish dairy herds

et al. 2004

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Bulk tank milk samples, collected from 347 herds throughout the Republic of Ireland using a sampling frame based on seven milk-recording organisations, were tested by ELISA for antibodies to Leptospira interrogans serovar hardjo. These herds, which had not been vaccinated against leptospirosis within the previous five years, were categorised according to their province, milk-recording organisation and size. Two-hundred-and-seventy-three herds (79%) had a positive ELISA titre. Both the probability of a herd being seropositive and the antibody level in the herd milk sample were affected by the province (P < 0.05 and P < 0.01, respectively) and the herd size category (P < 0.05 and P < 0.01, respectively). Larger herds were significantly more likely to have positive reactions and higher mean concentrations of antibody. It was concluded that a high proportion of unvaccinated Irish dairy herds have been exposed to infection with Leptospira hardjo.

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Restriction endonuclease analysis of Leptospira interrogans serovar hardjo isolates from cattle

Cited by 49 publications

References 17 publications

Low-stringency single specific primer PCR for identification of Leptospira

Low-stringency single specific primer PCR for identification of Leptospira

Molecular typing of Leptospira interrogans serovar Hardjo isolates from leptospirosis outbreaks in Brazilian livestock

Prevalence of antibodies to Leptospira interrogans serovar hardjoin bulk tank milk from unvaccinated irish dairy herds

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