Restriction endonuclease mapping of the fowlpox virus genome

Coupar, Barbara E.H.; Teo, Tian Seng; Boyle, David B.

doi:10.1016/0042-6822(90)90285-y

Cited by 48 publications

(27 citation statements)

References 34 publications

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“…The FPV genome was assembled into a contiguous sequence of 288,539 bp, which is slightly smaller in size than previous estimates of 299 to 309 kbp for low-passage-number FPV field strains (45,115). Because the hairpin loops were not sequenced, the left-most nucleotide of the assembled sequence was arbitrarily designated base 1.…”

Section: Resultsmentioning

confidence: 99%

“…The FPV genome, containing 260 to 309 kbp of doublestranded DNA, is larger than other described chordopoxvirus (ChPV) genomes (45,115,120). Past work on FPV genomics, much of which used highly tissue culture-passaged FPV strains, has provided genetic information on approximately one-third of the viral genome, including some viral genes with putative immune evasion and host range functions (16,18,20,57,93,127,150,163,166,191).…”

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confidence: 99%

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The Genome of Fowlpox Virus

Afonso

Tulman

et al. 2000

J Virol

305

312

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

The Genome of Fowlpox Virus

Afonso

Tulman

et al. 2000

J Virol

305

312

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“…Precipitated DNA was spooled out, dried briefly under vacuum and resuspended with 10 mM Tris-HC1 pH 7.5, to a concentration of 0.5 to 1.0 pg ml-'. Restriction enzyme digests, agarose gel electro-phoresls and field inversion gel electrophoresis were carried out as described in Coupar et al (1990).…”

Section: Cells and Viruses Bluegill Fry (Bf-2 Atcc CCL 91)mentioning

confidence: 99%

Comparison of epizootic haematopoietic necrosis and Bohle iridoviruses, recently isolated Australian iridoviruses

Hengstberger¹,

Hyatt²,

Speare³

et al. 1993

Dis. Aquat. Org.

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ABSTRACT. Epizootic haematopoletic necrosis virus (EHNV) and Bohle virus (BIV) are piscine and amphibian iridoviruses respectively which have been isolated in Australia from redfin perch Perca fluviatjlis L., cultured rainbow trout Oncorhynchus mykiss (Walbaum) and the ornate burrowing frog Limnodynastes ornatus (Gray). To determine whether the viruses were similar they were examined at the ultrastructural, protein, antigenic and genomic levels. EHNV was found to differ from BIV in cytopathic effect on host cells, size, site of virus release, molecular weights of proteins and restriction endonuclease digest profiles of the respective DNA's. On the other hand both iridoviruses \yere also found to infect a similar range of tissue culture cells, exhibit a similar sequence of ultrastuctural events and possess proteins of similar antigenic reactivity. DNA from EHNV and BIV cross hybridized to a high level. When the BIV polypeptides were compared to the published data for purified frog virus 3 (FV3) and with the appropriate restriction enzyme profiles, a similarity was noted between the 2 viruses. This implies that EHNV, which shares many structural and biochemical characteristics with BIV, is itself related to the Ranavirus genus of iridoviruses.

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“…The virus replicates in the cytoplasm of infected cells (Moss et al, 1990). It contains a double-stranded DNA genome of approximately 260 to 300 kbp (Muller et al, 1978;Coupar et al, 1990;Mockett et al, 1992;Afonso et al, 2000). The mature virus is brick shaped, measures about 330-280-200 nm, and has an outer coat composed of random arrangements of surface tubules (Tripathy and Reed, 2003).…”

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confidence: 99%

Poxvirus infection in Hungarian great tits ( Parus major ): Case report

Palade

Biró²,

Dobos-Kovács

et al. 2008

Acta Veterinaria Hungarica

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From a total of 1819 great tits (Parus major) ringed in 2007 in Pilis Mountains, Hungary, 15 birds presented nodular proliferative lesions on different areas of the head and eyelids, suggesting a poxvirus infection. Three birds were submitted for analysis. The presence of avipoxvirus infection was confirmed by histopathology, electron microscopy (EM) and a polymerase chain reaction (PCR) based technique. Nucleotide sequence analysis of a 428 base pairs (bp) fragment of the viral 4b core protein gene revealed 100% identity between two of the Hungarian isolates (PM9 HUN, PM33 HUN) and two great tit poxvirus strains isolated in Norway in 1973 (GTV A256, GTV A311). The third Hungarian isolate (PM34 HUN) was more closely related to a different Norwegian isolate (GTVA310) than to the Hungarian isolates. The nucleotide sequence analysis of a shorter fragment of the viral 4b core protein (227 bp) gene revealed 100% identity between the Hungarian isolates, the same Norwegian isolates and a great tit poxvirus strain detected in Austria in 2007.

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Restriction endonuclease mapping of the fowlpox virus genome

Cited by 48 publications

References 34 publications

The Genome of Fowlpox Virus

The Genome of Fowlpox Virus

Comparison of epizootic haematopoietic necrosis and Bohle iridoviruses, recently isolated Australian iridoviruses

Poxvirus infection in Hungarian great tits ( Parus major ): Case report

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