Restriction endonucleases whose sites are predictable from the amino acid sequence offer an improved strategy for typing bovine rotaviruses

Hussein, Heba; Frost, Éric; Deslandes, Sylvie; Talbot, Brian G.; Elazhary, Youssef

doi:10.1006/mcpr.1997.0128

Cited by 3 publications

(15 citation statements)

References 18 publications

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“…The well-characterized cell culture-adapted BRV strains NCDV (G6,P [1]), UK (G6,P [5]), B223 (G10,P [11]), and 678 (G8,P [5]) (3) were used to confirm the specificity of the reverse transcription-PCR (RT-PCR) typing assay.…”

Section: Virusesmentioning

confidence: 99%

“…The upstream generic primer on the VP7 gene and three specific G-typing primers (Table 1) were used in a second round of PCR amplification for the characterization of the G6, G8, and G10 serotypes. Similarly, the upstream generic primer on the VP4 gene and three specific P-typing primers (Table 2) were used in a second amplification for the characterization of the P [1], P [5], and P [11] serotypes. RT-PCR of full-length VP7 and partial-length VP4 genes.…”

Section: Virusesmentioning

confidence: 99%

“…Molecular methods such as methods that use specific probes (13,17) or type-specific PCR primers (8,14) and restriction endonuclease analysis (1,6,11) have been shown to be a solution to the disadvantages encountered by serological methods and, in recent years, have largely been used in epidemiological studies carried out worldwide.…”

mentioning

confidence: 99%

“…Field surveys have demonstrated that, so far, G6 and G10 are the most prevalent G serotypes among calf strains worldwide, whereas G8 is the least common serotype worldwide (1,17,18). According to the available evidence, P [5] and P [11] represent the most common P serotypes occurring in cattle; conversely, P [1] strains have very rarely been detected in the field (1,17).…”

mentioning

confidence: 99%

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Determination of Bovine Rotavirus G and P Serotypes in Italy by PCR

et al. 1999

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Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55.7% of the samples; viruses of the G10 and G8 serotypes were found in 34.9 and 4.7% of the samples, respectively. The virus in one sample (0.7%) was not classified due to concomitant infection with G6 and G8 strains, whereas viruses in six samples (4.0%) could not be characterized with any of the three G serotype-specific primers selected for the present study. When examined for their P-serotype specificities, viruses in 55 and 42.3% of the samples were characterized as P[11] and P[5], respectively, no P[1] serotype was identified, and viruses in 2.7% of the samples could not be classified due to multiple reactivity with both P[5]- and P[11]-specific primers. Various combinations of G and P serotypes were observed, the most frequent being G6,P[5] (38.3%), G10,P[11] (31.5%), and G6,P[11] (15.4%). The results of the present study, while contributing to a better understanding of the epidemiology of bovine rotaviruses in Italy, address the relevance of serotype specificity with regard to the constancy of the quality of bovine rotavirus vaccines under different field conditions.

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Section: Virusesmentioning

confidence: 99%

Section: Virusesmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Determination of Bovine Rotavirus G and P Serotypes in Italy by PCR

et al. 1999

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“…A variety of RT-PCR-based genotyping assays have been developed since the early 1990s. These assays target primarily the VP7 and VP4 genes and, more recently, the NSP4 gene (Gouvea et al, 1990(Gouvea et al, , 1994aGentsch et al, 1992;Das et al, 1994;Ray et al, 2003;Rodríguez-Díaz et al, 2008), although RT-PCR amplification combined with either RFLP analysis or nucleotide sequencing of various genes has also been adapted for strain characterization in various laboratories (Gouvea et al, 1993;Iizuka et al, 1993;Chang et al, 1996;Dubois et al, 1997;Hussein et al, 1997;Kudo et al, 2001;O'Halloran et al, 2002;DiStefano et al, 2005). A combination of typing methods based on a variety of genes provides a means of characterizing group A rotavirus strains of most host species and generates insight into the genetic diversity and ecology of these viruses.…”

Section: Introductionmentioning

confidence: 99%

Assignment of the group A rotavirus NSP4 gene into genotypes using a hemi-nested multiplex PCR assay: a rapid and reproducible assay for strain surveillance studies

Bányai

Bogdan

Szücs

et al. 2009

Journal of Medical Microbiology

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The rotavirus non-structural protein NSP4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. The NSP4 gene has been classified into six genotypes (A-F). A seminested triplex PCR was developed for genotyping the major human NSP4 genotypes (A-C), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. A total of 192 previously characterized human strains representing numerous G and P type specificities (such as G1P [8] [7]) were also successfully NSP4-typed. Four human G3P[9] strains and one feline G3P [9] strain were found to possess an NSP4 A genotype, instead of NSP4 C, suggesting a reassortment event between heterologous strains. Routine NSP4 genotyping may help to determine the genomic constellation of rotaviruses of man and livestock, and identify interspecies transmission of heterologous strains.

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