Restriction fragment length polymorphism analysis of Fusobacterium necrophorum using a novel repeat DNA sequence and a 16S rRNA gene probe

Hodgson, A

doi:10.1016/0378-1097(93)90311-o

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…34 There are three animal biovars of F. necrophorum. Type A, which will agglutinate chicken, human, and pigeon erythrocytes and is highly virulent, causing bovine hepatic abscesses 35 , Type AB, which is associated with bovine and ovine foot abscesses, and Type B, which is least virulent and can be isolated from the rumen of animals and is also found within lesions caused by the A or AB biotypes. 35 There are no convenient methods for distinguishing between isolates within the three biotype categories.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Falkler¹,

Enwonwu²,

Idigbe³

1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. A study of the predominant microflora in active sites of noma (cancrum oris) lesions was carried out in eight noma patients 3-15 years of age in Sokoto State in northwestern Nigeria. Paper point sampling and conventional anaerobic microbiologic techniques were used. Fusobacterium necrophorum was recovered from 87.5% of the noma lesions. Oral microorganisms included Prevotella intermedia, alpha-hemolytic streptococci, and Actinomyces spp. which were isolated from 75.0%, 50.0%, and 37.5% of the patients, respectively. Peptostreptococcus micros, Veillonella parvula, Staphylococcus aureus, and Pseudomonas spp. were each recovered from one lesion. The F. necrophorum and P. intermedia isolates were tested for antibiotic sensitivity to clindamycin, tetracycline, metronidazole, and penicillin using the E-test, and all strains were observed to be sensitive to all of the antibiotics tested with the exception of one strain of P. intermedia, which showed resistance to penicillin. The first reported isolation from human noma lesions of F. necrophorum, a pathogen primarily associated with animal diseases, may have important etiologic and animal transmission implications.Noma (cancrum oris) is a destructive gangrenous stomatitis occurring mainly in children. It may lead to devastating facial deformity, circumferential scarring, stenosis of the mouth, and in many cases death. This disease occurs almost exclusively among poor malnourished children in developing countries.

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Section: Discussionmentioning

confidence: 99%

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Falkler¹,

Enwonwu²,

Idigbe³

1999

The American Journal of Tropical Medicine and Hygiene

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“…Using seven strains of biotype AB (virulent) and one strain of biotype B (avirulent) (F. necrophorum subsp. funduliforme) originating from ovine foot abscesses or interdigital foot lesions, Hodgson et al (15) reported on restriction fragment length polymorphism analysis of F. necrophorum using a novel repeat DNA sequence (insertion elements) and a 16S rRNA gene probe. The repeated DNA sequence probe was shown to be sensitive and capable of distinguishing between F. necrophorum isolates having sequence homology with the 16S rRNA gene probe; however, the repeat DNA sequence probe failed to hybridize to DNA from biotype B of F. necrophorum, indicating absence of that particular sequence.…”

Section: Discussionmentioning

confidence: 99%

Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses

Okwumabua

Tan

Staats

et al. 1996

Appl Environ Microbiol

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Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fusobacterium necrophorum subsp. necrophorum. Ruminal F. necrophorum subsp. necrophorum had low leukotoxin titers yet was virulent in mice. Fusobacterium necrophorum subsp. funduliforme of hepatic or ruminal origin had no hemagglutination activity, had low hemolytic and leukotoxic activities, and was less virulent to mice. For ribotyping, chromosomal DNAs of 10 F. necrophorum subsp. necrophorum and 11 F. necrophorum subsp. funduliforme isolates were digested with restriction endonucleases (EcoRI, EcoRV, SalI, PstI, and HaeIII) and examined by restriction fragment length polymorphisms after hybridizing with a digoxigenin-labeled cDNA probe transcribed from a mixture of 16 and 23S rRNAs from Escherichia coli. The most discriminating restriction endonuclease enzyme for ribotyping was EcoRI. The presence or absence of two distinct bands of 2.6 and 4.3 kb differentiated the two subspecies. Regardless of the origin, only F. necrophorum subsp. necrophorum, a virulent subspecies, had a ca. 2.6-kb band, whereas F. necrophorum subsp. funduliforme, a less virulent subspecies, had a ca. 4.3-kb band. Ribotyping appears to be a useful technique to genetically differentiate the two subspecies of F. necrophorum. Fusobacterium necrophorum, a gram-negative, pleomorphically rod-shaped, and anaerobic bacterium, is associated with a wide variety of necrotic lesions (necrobacillosis) in animals and humans (16, 18, 20). It is the primary etiologic agent of liver abscesses and foot rot in feedlot cattle (11, 20, 29). Two major biotypes or subspecies of F. necrophorum-biotype A, or Fusobacterium necrophorum subsp. necrophorum, and biotype B, or Fusobacterium necrophorum subsp. funduliforme-are involved in liver abscesses of cattle (20, 29). The classification is based on cellular and colonial morphology, growth characteristics in a broth medium, toxin (leukotoxin and hemolysin) production, virulence in mice, and ability to agglutinate chicken erythrocytes (4, 21, 31, 38). Also, differences in the production of certain extracellular enzymes (DNase, alkaline phosphatase, and lipase) have been reported (2, 30, 38). However, in some instances, the difference between the two subspecies, particularly in relation to biological activity, was less defined (38). Differences at the genetic level between the two subspecies based on DNA-DNA homology have been reported (31). However, this method lacks the ability to differentiate strains within a given subspecies and is not useful for epidemiological purposes. DNA restriction fragment length polymorphism analysis, using a novel repeat of a DNA sequence and a 16S rRNA gene probe, of F. necrophorum isolates of foot rot origin has been reported (15). However, in that study, F.

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“…General features. The IS91 family is composed of eight members, of which one, ISFn1, has been only partially determined (145) and three are isoforms. Two additional members, IS92L and R form part of a composite transposon.…”

Section: Is91 Familymentioning

confidence: 99%

Insertion Sequences

Mahillon

Chandler

1998

Microbiol Mol Biol Rev

1,275

831

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SUMMARY Insertion sequences (ISs) constitute an important component of most bacterial genomes. Over 500 individual ISs have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. The last 10 years have also seen some striking advances in our understanding of the transposition process itself. Not least of these has been the development of various in vitro transposition systems for both prokaryotic and eukaryotic elements and, for several of these, a detailed understanding of the transposition process at the chemical level. This review presents a general overview of the organization and function of insertion sequences of eubacterial, archaebacterial, and eukaryotic origins with particular emphasis on bacterial elements and on different aspects of the transposition mechanism. It also attempts to provide a framework for classification of these elements by assigning them to various families or groups. A total of 443 members of the collection have been grouped in 17 families based on combinations of the following criteria: (i) similarities in genetic organization (arrangement of open reading frames); (ii) marked identities or similarities in the enzymes which mediate the transposition reactions, the recombinases/transposases (Tpases); (iii) similar features of their ends (terminal IRs); and (iv) fate of the nucleotide sequence of their target sites (generation of a direct target duplication of determined length). A brief description of the mechanism(s) involved in the mobility of individual ISs in each family and of the structure-function relationships of the individual Tpases is included where available.

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Restriction fragment length polymorphism analysis of Fusobacterium necrophorum using a novel repeat DNA sequence and a 16S rRNA gene probe

Cited by 4 publications

References 8 publications

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Isolation of Fusobacterium necrophorum from cancrum oris (noma).

Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses

Insertion Sequences

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