Restriction fragment length polymorphism analysis shows that the hippuricase gene of
            <i>Campylobacter jejuni</i>
            is highly conserved

Slater, E.; Owen, R. J.

doi:10.1046/j.1472-765x.1997.00218.x

Cited by 42 publications

(30 citation statements)

References 4 publications

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“…Strain 6871 was identified as an atypical (hippurate-negative) C. jejuni strain, according to PCR-RFLP analysis of the 23S rRNA gene and dot blot hybridization. Similar hippurate-negative C. jejuni strains have been shown to be quite uncommon (33,37). In one study, 1.6% of human and poultry strains were reported to be hippurate negative according to the results of quantitative DNA hybridizations and hippurate hydrolysis experiments (37).…”

Section: Discussionmentioning

confidence: 92%

“…In one study, 1.6% of human and poultry strains were reported to be hippurate negative according to the results of quantitative DNA hybridizations and hippurate hydrolysis experiments (37). In another study, some C. jejuni strains (less than 1%) did not produce a PCR product for the hippuricase gene (33). Hippurate-negative strains producing a hippuricase PCR product smaller than expected have also been reported (6,35).…”

Section: Discussionmentioning

confidence: 95%

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Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

2004

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The phylogeny of 12 Campylobacter species and reference strains of Arcobacter butzleri and Helicobacter pylori was studied based on partial 593-bp groEL gene sequences. The topology of the phylogenetic neighbor-joining tree based on the groEL gene was similar to that of the tree based on the 16S rRNA gene. However, groEL was found to provide a better resolution for Campylobacter species, with lower interspecies sequence similarities (range, 65 to 94%) compared with those for the 16S rRNA gene (range, 90 to 99%) and high intraspecies sequence similarities (range, 95 to 100%; average, 99%). A new universal reverse primer that amplifies a 517-bp fragment of the groEL gene was developed and used for PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of 68 strains representing 11 Campylobacter species as well as reference strains of A. butzlerii and H. pylori. Digestion with the AluI enzyme discriminated all Campylobacter species included in the study but showed more intraspecies diversity than digestion with the ApoI enzyme. A hippurate-negative variant of Campylobacter jejuni with a high level of groEL sequence similarity to both C. jejuni (96%) and C. coli (94%) gave a unique AluI profile and an ApoI profile identical to those of other C. jejuni strains. In conclusion, groEL gene sequencing and PCR-RFLP analysis are recommended as valuable tools for the identification of Campylobacter species.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

2004

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“…Then, 100 l of the final enrichment broth was plated on Karmali agar and incubated in a microaerobic atmosphere at 42°C for 48 h. Plates that were negative for Campylobacter at that time were reincubated for an additional 24 h. Isolates were identified to the species level by routine phenotyping methods (32) and by two PCR methods (7,14). Isolates which had a hippurate-negative phenotype but in which a hippurate gene was detected by PCR were identified as C. jejuni (38).…”

Section: Methodsmentioning

confidence: 99%

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

et al. 2008

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Molecular strain typing is essential for deciphering the epidemiology of Campylobacter jejuni infections. We applied two different methods, multilocus sequence typing (MLST) and analysis of the flaA short variable repeat (SVR), to 289 isolates (163 human, 56 chicken, 34 raw milk, and 36 environmental water isolates) collected in the province of Québec, Canada, over 3 years; in addition, the analysis included the pulsed-field gel electrophoresis (PFGE) typing results for a subset of 131 isolates studied previously. MLST defined 96 sequence types (STs) and 20 clonal complexes (CCs), including 49 STs (73 isolates, 25%) and 39 alleles not previously documented in an international database. The frequency of new STs was significantly higher among water isolates than among isolates from other sources (18/36 [50%] and 55/253 [22%], respectively; P < 0.001). Nine of the 10 most prevalent CCs included isolates from humans and at least one other source; five CCs comprised exclusively or mostly human and chicken isolates. However, water and milk were the predominant nonhuman sources among the remaining CCs, suggesting that sporadic C. jejuni infections in humans may frequently arise from sources other than chickens. All three typing systems were discriminatory (discriminatory index > 0.9). Among 131 isolates analyzed by PFGE, each of the 20 types represented by two or more isolates corresponded to a single CC. In contrast, among the 14 most prevalent types detected by analysis of the flaA SVR (5 to 27 isolates each), 8 (57%) included isolates that represented multiple different CCs. The basis for these discordant results was uncertain. Antimicrobial resistance was randomly distributed among the CCs and appeared to be more closely related to the source of an isolate than its genotype. Although MLST is labor-intensive and expensive, it remains the single best method for the genotyping of C. jejuni isolates and deciphering the epidemiologic relationships among isolates.

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“…C. jejuni isolates are hippurate positive, although atypical isolates, which are phenotypically negative but have the hippuricase gene, have been described. Speciation was established by detection of the hippuricase gene by PCR (30) and confirmed by a 23S rRNA PCR restriction fragment length polymorphism method (10).…”

Section: Molecular Identificationmentioning

confidence: 99%

Detection of Heat-Stable Antigens of Campylobacter jejuni and C. coli by Direct Agglutination and Passive Hemagglutination

et al. 2002

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The two serotyping schemes for the detection of heat-stable antigens of Campylobacter jejuni and Campylobacter coli use the same strains for antiserum production but differ in the detection systems used for identifying agglutination. The Penner method uses passive hemagglutination (PHA) while the Laboratory of Enteric Pathogens method uses the same antisera but in a whole-bacterial-cell direct agglutination (DA) protocol. C. jejuni produces a polysaccharide capsule, which is antigenic, and is the main component detected by the PHA method. The DA method will detect both capsule antigens and lipopolysaccharide (LPS) or lipooligosaccharide (LOS) surface antigens. Comparison of both methods by using a selection of isolates from human infection has shown a range of variation in agglutination specificity, reflecting the differences in antigens detected by the two methods. While 27.4% of the 416 C. jejuni isolates reacted with the antisera raised against the same type strains by either method, the majority showed a range of more complex relationships. None of the 37 C. coli isolates reacted with the same antiserum by both methods. Together the two schemes gave a total of 102 distinct combined serogroups for C. jejuni and 16 for C. coli. Thus, while some clonally related isolates share the same capsule and LOS or LPS antigens, other strains appear to have a common capsule antigen but differ in their LPS or LOS structures or vice versa.

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Restriction fragment length polymorphism analysis shows that the hippuricase gene of Campylobacter jejuni is highly conserved

Cited by 42 publications

References 4 publications

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences

Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada

Detection of Heat-Stable Antigens of Campylobacter jejuni and C. coli by Direct Agglutination and Passive Hemagglutination

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