Restriction Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis Isolated from Countries in the Western Pacific Region

Abstract

A total of 422 Mycobacterium tuberculosis isolates from eight countries were subjected to IS 6110 and IS 1081 DNA fingerprinting by means of restriction fragment analysis to characterize M. tuberculosis strains from each country. Chinese, Mongolian, Hong Kong, Filipino, and Korean isolates had comparatively more copies of IS 6110 (proportion with eight or more copies; 95% ± 5%), while Thai, Malaysian, and Vietnamese isolates had fewer copies (proportion with eight or more copies, 60% ± 4%). We found a number of novel IS 1081 types in this study. One IS 1081 type was present in 56% of Filipino isolates, had a specific 6.6-kb Pvu II fragment in its IS 6110 DNA fingerprint, and was termed the “Filipino family.” The IS 1081 types of Thai isolates had interposing characteristics between the characteristics of northeastern Asian and southeastern Asian IS 1081 types. A 1.3-kb single-copy IS 6110 fragment was found only in Vietnamese M. tuberculosis isolates. Although M. tuberculosis isolates from each country had comparatively similar characteristics depending on the classification factor, each country's isolates showed characteristic DNA fingerprints and differed slightly from the isolates from the other countries in either the mode number of IS 6110 copies or the distribution of IS 1081 types.

Mycobacterium tuberculosisBeijing Genotype Strains Associated with Febrile Response to Treatment

“…Worldwide, DNA fingerprinting has revealed extensive heterogeneity of M. tuberculosis genotypes (13). However, a distinct and predominant M. tuberculosis genotype, termed Beijing, has been found in the People's Republic of China and neighboring countries (1,14). Our study demonstrates that Beijing strains are also present in the Indonesian archipelago.…”

Characterization of mutations in drug resistant tuberculosis and diagnostic challenges in referral health facilities, Tanzania

“…Advances in genetic techniques have facilitated the better understanding of many epidemiological and bio-physiological attributes of the pathogen. The recent introduction of genotyping methods like IS6110 Restriction Fragment Length Polymorphism (Park at al., 2000), Spacer Oligonucleotide Typing (Spoligotyping) and mycobacterial interspersed repetitive units (Sola et al, 2003;Supply et al, 2001) has been useful in understanding molecular epidemiology of mycobacteria as they generate new insight regarding transmission pathways, relapse, and re-infection of TB (Alland et al, 1994;Small et al, 1994a). The general principle is that patients infected with clustered strains are epidemiologically linked (Alland et al, 1994;Kamerbeek et al, 1997;Small et al, 1994a).…”