Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Olsen, John Elmerdahl; Larsen, J. L.

doi:10.1128/aem.56.10.3130-3132.1990

Cited by 40 publications

(22 citation statements)

References 14 publications

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“…Comprehensive work has been done to clarify the relatedness of V. anguillarum and V. ordalii Kaper et al 1983;Larsen 1990;Grisez et al 1991;Pazos et al 1993;Alsina et al 1994). These works have shown a clear phenotypic and hence, taxonomic separation between these two species.…”

Section: Discussionmentioning

confidence: 99%

“…ordalii, as well as comparing these two species. Biochemical characterization (Kaper et al 1983;Larsen 1990;Grisez et al 1991 ;Pazos et al 1993;Alsina et al 1994) and serogrouping (Kitao et al 1983;SBrensen and Larsen 1986;Tajima et al 1986a, b) have probably been the most commonly used methods until now. In the last decade, analysis of bacterial DNA-plasmids as well as chromosomal DNA-has become prominent.…”

Section: Introductionmentioning

confidence: 99%

“…anguillarum serovar 0 2 strains have no plasmids, and a plasmid of 3&35 kb has been found in all V . ordalii strains Olsen and Larsen 1990). Hence, plasmid profiling alone is not an ideal method for epidemiological studies of these two species.…”

Section: Introductionmentioning

confidence: 99%

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Ribotyping and plasmid profiling of Vibrio anguillarum serovar O2 and Vibrio ordalii

Tiainen¹,

Pedersen²,

Larsen³

1995

Journal of Applied Bacteriology

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One hundred and twenty-nine strains of Vibrio anguillarum serovar O2 and 14 strains of Vibrio ordalii were ribotyped and examined for plasmid contents. A total of 35 different ribotypes were detected. The V. anguillarum serovar O2 strains were divided into 32 different ribotypes. The V. ordalii strains showed three different ribotypes, clearly distinct from those of the V. anguillarum strains. Ribotypes were separated into seven clusters, of which one comprised the V. ordalii strains. Clustering of the strains indicated a genetic difference between North European and South European V. anguillarum O2 strains. Sero-subgroups O2a and O2b shared ribotypes; however, three of the clusters did not include O2a strains. All V. ordalii strains had a plasmid of 32 kb. This plasmid was not detected in any of the V. anguillarum strains. Seventeen different plasmid profiles with 17 different sized plasmids were detected among the V. anguillarum strains. Most of the plasmids were small (< 6 kb) and found in several strains. Except for one South European strain, plasmids were detected only in the North European strains of V. anguillarum O2.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ribotyping and plasmid profiling of Vibrio anguillarum serovar O2 and Vibrio ordalii

Tiainen¹,

Pedersen²,

Larsen³

1995

Journal of Applied Bacteriology

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“…Following lysis, phenol-chloroform-isoamyl alcohol (25:24:1) was added and the samples were mixed well. After centrifugation, a sample of the supernatant was electrophoresed and plasmids were visualized essentially as described previously (21). V. cholerae O1 V 1057/25 and V 1075/25, each containing an approximately 150-kb plasmid, were used as control strains (27).…”

Section: Bacterial Strainsmentioning

confidence: 99%

Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

et al. 1996

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In the present study, 19 strains of Vibrio cholerae O1 biotype El Tor isolated during outbreaks of cholera in Guinea-Bissau in 1987, 1994, and 1995 were characterized to investigate a possible epidemiological relationship among the isolates. On the basis of ribotyping with the restriction enzyme BglI, 5 strains isolated in 1987 showed two closely related ribotypes, while 14 strains isolated in 1994 and 1995 showed the same ribotype that was distinct from the ribotypes of strains isolated in 1987. Southern blot hybridization of BglI-digested genomic DNA with a cholera toxin probe demonstrated that the strains isolated in 1987 showed an identical cholera toxin genotype, whereas O1 strains isolated in 1994 and 1995 showed the same genotype that was distinct from the genotype of strains isolated in 1987. These results were supported by the results of antibiotic susceptibility testing, in which strains isolated in 1987 showed resistance to polymyxin B only, while each of the strains from 1994 and 1995 showed resistance to polymyxin B, trimethoprim-sulfamethoxazole, and the vibriostatic agent O/129. Although our results are based on a limited number of V. cholerae O1 strains, they suggest that the epidemic in Guinea-Bissau in 1994 and 1995 was due to the introduction of a new strain to the country.

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“…In recent years, restriction fragment length polymorphism (RFLP) of plasmids has become widespread used both in epidemiological studies and the differentiation of several bacterial strains within a species such as Vibrio anguillarum (OLSEN and LARSEN 1990), V. ordalii (PEDERSEN et al 1996) andV. vulnificus (LEWIN et al 2000).…”

mentioning

confidence: 99%

Characterization by restriction fragment length polymorphism and plasmid profiling of Vibrio tapetis strains

Chevalier

Boulay

Paillard

2003

J. Basic Microbiol.

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A total of nine Vibrio tapetis strains acquired from France, the United Kingdom and Norway were studied for their plasmid content. All the isolates contained from two to four large plasmids, ranging from approximately 60 to 100 kpb. in size. These plasmids were subsequently subjected to restriction fragment length polymorphism analysis (RFLP). Using the EcoRI enzyme, three different restriction patterns were demonstrated, two of which were closely related. On the basis of RFLP patterns, the strain from Norway differed noticeably from the French and British strains.

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Restriction fragment length polymorphism of the Vibrio anguillarum serovar O1 virulence plasmid

Cited by 40 publications

References 14 publications

Ribotyping and plasmid profiling of Vibrio anguillarum serovar O2 and Vibrio ordalii

Ribotyping and plasmid profiling of Vibrio anguillarum serovar O2 and Vibrio ordalii

Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

Characterization by restriction fragment length polymorphism and plasmid profiling of Vibrio tapetis strains

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