Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregate

Varga, János; Kevei, Ferenc; Fekete, Csaba; Coenen, A.; Kozakiewicz, Z.; Croft, J. H.

doi:10.1016/s0953-7562(09)81286-0

Cited by 77 publications

(66 citation statements)

References 16 publications

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“…Species boundaries of organisms without a known sexual state are not always clear, and rely on a combination of these techniques to assign strains to the different species (Varga et al 1993;Parenicova´et al 1997;Parenicova´et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Aspergillus vadensis, a new species of the group of black Aspergilli

Vries

Frisvad

Vondervoort

et al. 2005

Antonie Van Leeuwenhoek

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A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the b-tubulin sequences of the individual strains. The new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone B, nigragillin, asperazine and kotanins. RFLP analysis using three genes as probes also resulted in a unique pattern. These data indicate that the strain was closely related but not identical to Aspergillus foetidus, Aspergillus niger and Aspergillus tubingensis. It was therefore designated as a novel species and named Aspergillus vadensis.

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Section: Discussionmentioning

confidence: 99%

Aspergillus vadensis, a new species of the group of black Aspergilli

Vries

Frisvad

Vondervoort

et al. 2005

Antonie Van Leeuwenhoek

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“…Isopropanol was used to precipitate the nucleic acids, they were washed with 70 % ethanol and finally resupended in demi water or TE buffer (10 mM Tris/ 10 mM EDTA, pH 7.5). Restriction enzyme analyses were performed with both BglII and HaeIII for A. niger, A. tubingensis and A.brasiliensis haplotypes (Varga et al 1993(Varga et al , 1994 and with HaeIII or EcoRI for A. japonicus and relatives (Hamari et al 1997) and A. carbonarius and relatives (Kevei et al 1996).…”

Section: Mitochondrial Dna Rflp Characterisationmentioning

confidence: 99%

“…b A. niger mitochondrial RFLP haplotypes 1a-1d and A. tubingensis haplotypes 2a-2f as recognised by Varga et al (1993Varga et al ( , 1994. c jap, A. japonicus and direct relatives (Hamari et al 1997).…”

Section: Tannasesmentioning

confidence: 99%

“…Aspergillus sect. Nigri consists of at least eight species based on recent morphological, RAPD, mitochondrial and RFLP studies (A. carbonarius, A. japonicus, A. ellipticus, A. heteromorphus, A. aculeatus, A. niger, A. tubingensis, and A. brasiliensis) (Kusters-van Someren, Samson & Visser 1991, Me´gne´gneau, Debets & Hoekstra 1993, Varga et al 1993. All members of the section have characteristic dark brown to black conidia.…”

Section: Introductionmentioning

confidence: 99%

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Efficient degradation of tannic acid by black Aspergillus species

Diepeningen

Debets

Varga

et al. 2004

Mycological Research

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“…These two species have a high morphological similarity and A. tubingensis was considered as a subspecies of A. niger. The utilization of molecular methods allowed a better distinction of the A. niger group until 1991 (Kusters- Van Someren et al, 1991;Varga et al, 1993;Accensi et al, 1999; and the classification of A. niger and A. tubingensis as distinct species.…”

Section: A Carbonarius 1%mentioning

confidence: 99%

Molecular identification of Aspergillus spp. isolated from coffee beans

Magnani

Fernandes

Prete

et al. 2005

Sci. agric. (Piracicaba, Braz.)

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Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was the species most frequently detected, followed by A. ochraceus and A. carbonarius.

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Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregate

Cited by 77 publications

References 16 publications

Aspergillus vadensis, a new species of the group of black Aspergilli

Aspergillus vadensis, a new species of the group of black Aspergilli

Efficient degradation of tannic acid by black Aspergillus species

Molecular identification of Aspergillus spp. isolated from coffee beans

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