Restriction in the lytic efficiency of complement on different erythrocyte targets: A re-examination of the activities of horse C8 and C9

Yarnall, David P.; Boyle, Michael D. P.

doi:10.1016/0161-5890(86)90067-2

Cited by 4 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The complement system in horses and its role in protecting against invading pathogens have been poorly investigated. Early studies indicated that horses had a potent alternative complement pathway capable of readily lysing rabbit erythrocytes [ 27 ] but not sheep erythrocytes [ 28 ]. Thus, the efficacy of the complement system in horses might depend on the pathway activated and the target antigen.…”

Section: Discussionmentioning

confidence: 99%

Orally administered β-glucan improves the hemolytic activity of the complement system in horses

et al. 2021

View full text Add to dashboard Cite

Background and Aim: Immune-modulating molecules mainly act on innate immune cells, which are central to early defense against invading pathogens and contribute to developing adaptive immunity. Yeast-extracted β-glucan, a model immune-modulating molecule, is widely used in several animal species; however, its effect on horse immune parameters has not been thoroughly investigated yet. This study aimed to evaluate the effects of orally administered β-glucan on selected innate immune parameters in horses. Materials and Methods: Eighteen thoroughbred horses were assigned equally into three groups as follows: One control group (no β-glucan) and two β-glucan experimental groups (one received 125 mg and the other 2 g of β-glucan per day for 28 days). Blood samples were collected before and at the end of the experiment for hematological analysis, whole blood phagocytosis, respiratory burst assays, and to assess the serum lysozyme and complement hemolytic activities. Results: At the end of the experiment, significant decreases (p<0.05) in monocyte numbers were observed in the control horses (258.8±45.9 vs. 115.3±41.5) and in those fed 125 mg/day of β-glucan (208.8±72.3 vs. 99.2±60.7), whereas a significant increase in numbers was noted in the horses that were fed 2 g/day of β-glucan (303.5±45.8 vs. 429.8±86.0; p<0.05). The natural hemolytic activity of the complement was higher only in horses fed 2 g/day of β-glucan (p=0.018) compared to the other groups. The hemolytic activity in the classical pathway was higher in those fed 125 mg/day (p=0.0035) and 2 g/day of β-glucan (p=0.0001). Conclusion: β-glucan improves important innate immune parameters and might be fed to horses before stressful events.

show abstract

Section: Discussionmentioning

confidence: 99%

Orally administered β-glucan improves the hemolytic activity of the complement system in horses

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It is known that sheep red cells are not lysed by horse serum and that the terminal components of complement participate in this restriction phenomenon [14,22]. The composition of the cell membrane is known to interfere with the action of the complement in both homologous and heterologous systems.…”

Section: Discussionmentioning

confidence: 99%

“…The effect of the in vitro ageing process on the lysis of sheep erythrocytes by horse complement was analysed. These cells are resistant to lysis by horse serum due to inhibition at the MAC level [14,22]. We found that sheep erythrocytes become susceptible to horse complement after in vitro ageing at 4°C in whole blood or in buffer.…”

Section: Introductionmentioning

confidence: 99%

Effect of the In Vitro Ageing on the Restriction of Sheep Erythrocyte Lysis by Horse Serum

et al. 1991

View full text Add to dashboard Cite

Fresh sheep erythrocytes are resistant to lysis by horse serum but gradually became susceptible to this source of complement after storage (ageing) in the blood at 4 degrees C. This occurs faster when the fresh cells are stored in isotonic buffer. The supernatant of the buffer/red cell suspension stored at 4 degrees C for 10-15 days restrict lysis by horse serum when incubated back at 37 degrees C or 43 degrees C with the 'aged' sheep cells or fresh guinea pig red cells. In assays using fresh guinea pig erythrocytes this effect is described by reincubation of the cells in buffer, and is specific to horse serum when compared to human serum.

show abstract

Horse complement protein C9: Primary structure and cytotoxic activity

Esser¹,

Tarnuzzer²,

Tomlinson³

et al. 1996

Molecular Immunology

View full text Add to dashboard Cite

Restriction in the lytic efficiency of complement on different erythrocyte targets: A re-examination of the activities of horse C8 and C9

Cited by 4 publications

References 10 publications

Orally administered β-glucan improves the hemolytic activity of the complement system in horses

Orally administered β-glucan improves the hemolytic activity of the complement system in horses

Effect of the In Vitro Ageing on the Restriction of Sheep Erythrocyte Lysis by Horse Serum

Horse complement protein C9: Primary structure and cytotoxic activity

Contact Info

Product

Resources

About