Restriction Site Mapping of Adenovirus Types 9 and 15 and Genome Types of Intermediate Adenovirus 15/H9

Adrian, Thomas E.; Bastian, Boris C.; Wagner, Valentina

doi:10.1159/000150089

Cited by 11 publications

(12 citation statements)

References 14 publications

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“…HAdV-15/H9 was neutralized by both HAdV-15 and -29 antisera and showed weak cross-reactivity against HAdV-9 antiserum (23,36). HAdV-15/H9 was then isolated from the rectum, stools, throat, and eye in the United States and Europe in the 1970s and 1980s (1,2,36). However, the isolation of HAdV-15/H9 has become rarer since the 1990s.…”

Section: Discussionmentioning

confidence: 99%

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

et al. 2011

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For 4 months from September 2008, 102 conjunctival swab specimens were collected for surveillance purposes from patients across Japan suspected of having epidemic keratoconjunctivitis (EKC). Human adenovirus (HAdV) DNA was detected in 61 samples by PCR, though the HAdV type for 6 of the PCR-positive samples could not be determined by phylogenetic analysis using a partial hexon gene sequence. Moreover, for 2 months from January 2009, HAdV strains with identical sequences were isolated from five conjunctival swab samples obtained from EKC patients in five different regions of Japan. For the analyses of the 11 samples mentioned above, we determined the nucleotide sequences of the entire penton base, hexon, and fiber genes and early 3 (E3) region, which are variable regions among HAdV types, and compared them to those of other HAdV species D strains. The nucleotide sequences of loops 1 and 2 in the hexons of all 11 samples showed high degrees of identity with those of the HAdV type 15 (HAdV-15) and HAdV-29 prototype strains. However, the fiber gene and E3 region sequences showed high degrees of identity with those of HAdV-9, and the penton base gene sequence showed a high degree of identity with the penton base gene sequences of HAdV-9 and -26. Moreover, the complete genome sequence of the 2307-S strain, which was isolated by viral culture from 1 of the 11 samples, was determined. The 2307-S strain was a recombinant HAdV between HAdV-9, -15, -26, -29, and/or another HAdV type; however, the recombination sites in the genome were not obvious. We propose that this virus is a novel intertypic recombinant, HAdV-15/29/H9, and may be an etiological agent of EKC.

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Section: Discussionmentioning

confidence: 99%

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

et al. 2011

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“…AV9 has a small (0.3 kbp) fragment [3], which had not been detected previously [2]; it is also present in AV23 and 33. Another difference is that the E fragment of AV29 [2] was proved to be spurious; consequently the DNA pattern of AV29 is identical with that of AV10 and 30.…”

Section: Bglhmentioning

confidence: 75%

“…AV8 DNA has been mapped physically in our laboratory using the three enzymes (Adrian, Wolf, Lauer and Wigand, to be published), likewise AV9 and AV15 [3]. Furthermore, three restriction patterns of genome types of the intermediate strains AV15/H9 [3] correspond to the following prototypes which can, therefore, also be considered as physically mapped: HindlII pattern (2) =AV17, BgllI pattern (3)=AV10, and BamHI pattern (4)=AV10. Gomes and Niel [6] reported data on physical maps of AV9 and AV19 with HindlII and BamHI.…”

Section: Data and Evaluationmentioning

confidence: 99%

“…Hence it is likely that comigrating fragments of different types have an identical location on genomes. This was proved experimentally, e.g., for AV9, AV15, and AV15/H9 intermediate strains [3]. Therefore, we attempted to map the genomes of all AV prototypes by adaptation of the restriction fragments obtained with the endonucleases HindlII, BgllI, and BamHI, considering their molecular weights and comparing them with those types whose maps have been established by classical biochemical methods.…”

Section: Introductionmentioning

confidence: 99%

“…Until now, physical mapping has been done only with a few types and restriction enzymes [3,6,11] and Adrian et al (unpublished results on AV8). AV types of subgenus D show a high degree of DNA homology, determined by liquid hybridization [7].…”

Section: Introductionmentioning

confidence: 99%

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Restriction site mapping of adenovirus prototypes of subgenus D

Wigand

Adrian

1990

Med Microbiol Immunol

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Restriction site maps with the endonucleases HindIII, BglII, and Bam HI were elaborated for the majority of 23 adenovirus prototypes of subgenus D (AV8 to AV39). On the basis of four or five types which have been mapped by biochemical methods, the physical maps for other types were constructed by adapting restriction fragments of defined molecular weight, as the homology among types of the same subgenus is considerable. Prototypes of subgenus D shared several common restriction sites; different sites, however, were distributed at random over the genome. The gene loci for hexon and fiber did not show either higher or lower variability.

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Genome polymorphism of human adenoviruses of subgenus C

Adrian

1996

Archives of Virology

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The genome of adenovirus (AV) types 1, 2, and 5 is known to be very variable as evidenced by the great number of genome types described (36, 61, and 35 for AV1, 2, and 5 respectively). Physical maps were constructed for nearly all of the restriction or R-variants by biochemical methods and by adapting restriction fragments of defined molecular weight. The alterations found with seven DNA restriction endonucleases were mapped on the genome. Altered restriction sites, found for the genome types of each of the three serotypes, appeared to be randomly distributed over the genome, although mutations seemed to occur preferentially on distinct sites of the genome. Many strains, especially those of AV5, were genomically much more related with one another than with the prototype. This finding is compatible with the interpretation that the related strains were derived from an unknown common ancestor.

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Restriction Site Mapping of Adenovirus Types 9 and 15 and Genome Types of Intermediate Adenovirus 15/H9

Cited by 11 publications

References 14 publications

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

Complete Genome Analysis of a Novel Intertypic Recombinant Human Adenovirus Causing Epidemic Keratoconjunctivitis in Japan

Restriction site mapping of adenovirus prototypes of subgenus D

Genome polymorphism of human adenoviruses of subgenus C

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