Restructuring of Endophytic Bacterial Communities in Grapevine Yellows-Diseased and Recovered Vitis vinifera L. Plants

Bulgari, Daniela; Casati, P.; Crepaldi, P.; Daffonchio, Daniele; Quaglino, F.; Brusetti, Lorenzo; Bianco, P.A.

doi:10.1128/aem.00051-11

Cited by 95 publications

(77 citation statements)

References 13 publications

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“…The endophytic community in a single plant is usually composed by numerous and systematically diverse species of fungi (Petrini 1991;Oono et al 2015). Their number and species composition is influenced by factors such as the environment (Saona et al 2010;Yousaf et al 2010;N unez-Trujillo et al 2012), plant physiology (Islam et al 2010), anthropogenic factors (Rasche et al 2006), and pathogen infections (Araujo et al 2002;Bulgari et al 2011;Buyer et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

et al. 2016

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a b s t r a c tFungal endophytes present in different asymptomatic grapevine plants (Vitis vinifera L.) located in different vineyards within Alentejo, a highly important viticulture region in Portugal, were identified in this study. Sampled grapevine plants included the three most representative cultivars in the region, Syrah, Cabernet Sauvignon, and Aragonez, growing under two different modes of management, conventional and biological. Sixteen fungal taxa were identified through sequencing of the internal transcribed spacer region. Total number of endophytic fungi isolated showed significant differences both in management mode and in cultivars, with higher numbers in grapevines under conventional mode and from Syrah cultivar. The composition of fungal endophytic communities did not show significant differences among cultivars, but differences were observed between fungal communities isolated from grapevines under biological or conventional modes. The most fungal taxa isolated from grapevines cultivated under biological mode were Alternaria alternata, Cladosporium sp., and Nigrospora oryzae, and under conventional mode Botrytis cinerea, Epicoccum nigrum, and Epicoccum sp. These differences suggest that the different products used in grapevine production have impacts in fungal endophytic composition. Further investigation of the identified fungi with respect to their antagonistic characteristics and potential use in plant protection to ensure food safety is now in course.

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Section: Introductionmentioning

confidence: 99%

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

et al. 2016

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“…A growing number of studies have focused on understanding how the plant microbiome changes during disease development (15,17,(20)(21)(22)(23)(24)(25)(26). While results from these studies suggest that plant-associated microbial communities undergo perturbation during environmental change or disease progression, one may alternatively argue that the disease itself is a consequence of complex changes to the communities.…”

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confidence: 99%

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Blaustein

Lorca

Meyer

et al. 2017

Appl Environ Microbiol

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Stable associations between plants and microbes are critical to promoting host health and productivity. The objective of this work was to test the hypothesis that restructuring of the core microbiota may be associated with the progression of huanglongbing (HLB), the devastating citrus disease caused by Liberibacter asiaticus, Liberibacter americanus, and Liberibacter africanus. The microbial communities of leaves (n ϭ 94) and roots (n ϭ 79) from citrus trees that varied by HLB symptom severity, cultivar, location, and season/time were characterized with Illumina sequencing of 16S rRNA genes. The taxonomically rich communities contained abundant core members (i.e., detected in at least 95% of the respective leaf or root samples), some overrepresented site-specific members, and a diverse community of lowabundance variable taxa. The composition and diversity of the leaf and root microbiota were strongly associated with HLB symptom severity and location; there was also an association with host cultivar. The relative abundance of Liberibacter spp. among leaf microbiota positively correlated with HLB symptom severity and negatively correlated with alpha diversity, suggesting that community diversity decreases as symptoms progress. Network analysis of the microbial community time series identified a mutually exclusive relationship between Liberibacter spp. and members of the Burkholderiaceae, Micromonosporaceae, and Xanthomonadaceae. This work confirmed several previously described plant disease-associated bacteria, as well as identified new potential implications for biological control. Our findings advance the understanding of (i) plant microbiota selection across multiple variables and (ii) changes in (core) community structure that may be a precondition to disease establishment and/or may be associated with symptom progression.IMPORTANCE This study provides a comprehensive overview of the core microbial community within the microbiomes of plant hosts that vary in extent of disease symptom progression. With 16S Illumina sequencing analyses, we not only confirmed previously described bacterial associations with plant health (e.g., potentially beneficial bacteria) but also identified new associations and potential interactions between certain bacteria and an economically important phytopathogen. The importance of core taxa within broader plant-associated microbial communities is discussed.KEYWORDS core microbiome, plant-associated microbiota, Liberibacter, pathogenmicrobe interactions

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“…However, the relation between communities of endophytic fungi and host plants is still poorly studied, and as of yet, is far from being fully understood. In grapevines, recent studies have shed some light upon the bacterial endophytic communities (13,14,20,47,72), while investigations on fungal communities have been rare and often limited to culture-dependent methods (20,30,43,71). Furthermore, research has mainly focused on subsoil plant-microbe associations (36,65).…”

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confidence: 99%

“…Several factors may affect plant-associated microbial communities, e.g., anthropic factors (52,53), plant physiology (34), the environment (57,74), and pathogen infections (4,14,15). A shift in the composition of microbial communities associated with plants can be driven by genetic and physiological diversities, e.g., between different cultivated varieties (1,42,47).…”

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confidence: 99%

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Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management

Pancher

Ceol

Corneo

et al. 2012

Appl Environ Microbiol

165

127

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ABSTRACTWe studied the distribution of fungal endophytes of grapevine (Vitis viniferaL.) plants in a subalpine area of northern Italy, where viticulture is of high economic relevance. We adopted both cultivation-based and cultivation-independent approaches to address how various anthropic and nonanthropic factors shape microbial communities. Grapevine stems were harvested from several locations considering organic and integrated pest management (IPM) and from the cultivars Merlot and Chardonnay. Cultivable fungi were isolated and identified by internal-transcribed-spacer sequence analysis, using a novel colony-PCR method, to amplify DNA from fungal specimens. The composition of fungal communities was assessed using a cultivation-independent approach, automated ribosomal intergenic spacer analysis (ARISA). Multivariate statistical analysis of both culture-dependent and culture-independent data sets was convergent and indicated that fungal endophytic communities in grapevines from organically managed farms were different from those from farms utilizing IPM. Fungal communities in plants of cv. Merlot and cv. Chardonnay overlapped when analyzed using culture-dependent approaches but could be partially resolved using ARISA fingerprinting.

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Restructuring of Endophytic Bacterial Communities in Grapevine Yellows-Diseased and Recovered Vitis vinifera L. Plants

Cited by 95 publications

References 13 publications

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Fungal Endophytic Communities in Grapevines (Vitis vinifera L.) Respond to Crop Management

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