Result of attempted hematopoietic reconstitution using isologous, peripheral blood mononuclear cells: a case report

Abstract

In order to test the effect of peripheral blood mononuclear cell infusions on hematopoietic recovery in man we intensively leukapheresed a normal identical twin and obtained 9.8 x 10(10) peripheral blood mononuclear cells containing 4 x 10(5) CFU-C. These isologous cells were infused into his identical twin brother who had received 150 rad of total body irradiation and intensive combination chemotherapy as adjuvant therapy for Ewing's sarcoma. When compared to other patients receiving similar treatment, leukocyte recovery was accelerated by 3–4 wk, and occurred at a rate comparable to that induced by infusion of autologous cryopreserved marrow. Recovery of granulocytes, monocytes and platelets was not accelerated. The low number of CFU-C present in the preparation used ((one-eighth the number of CFU-C we usually obtain from bone marrow autograft collections) may have led to the pattern of hematopoietic recovery we observed in this patient.

Megakaryocytic cells in mixed haemopoietic colonies (CFU‐GEMM) from the peripheral blood of normal individuals

“…This is supported by similar findings for the mouse pluripotent progenitor cells CFUs, with circulating CFUs having a lower proliferation potential than their counterparts in the bone marrow (Micklem et al, 1975). Similarly the inferior repopulating ability of circulating as compared to bone marrow-derived normal human pluripotent haemopoietic progenitor cells in the treatment of marrow aplasia (Hershko et al, 1979;Abrams et al, 1980) could be explained by the relative lack of multipotent progenitor cells in peripheral blood found in this study.…”

High levels of circulating haemopoietic stem cells in very early remission from acute non‐lymphoblastic leukaemia and their collection and cryopreservation

“…The two reported attempts at haemopoietic reconstitution in man using peripheral blood cells from normal donors have failed (Hershko et al, 1979;Abrams et al, 1980), probably because an inadequate number of stem cells, as indicated by the CFU-GM content, was collected and infused. A preliminary study on an ANLL patient using discontinuous leukapheresis during early remission has been reported (Juttner et al, 1982b) and 4 x lo4 CFU-GM/kg body weight were cryopreserved but no systematic studies on the feasibility and safety of the procedure have been reported.…”

Blood stem cell transplantation: From preclinical to clinical models

“…(1 970) [75], Hershko et al (1979) [76] or Abrams et al (1980) [77] were not or only partially successful may well be due to the conditioning regimen on the one hand, and to the insufficient number and quality of stem and progenitor cells collected and transfused on the other. It, therefore, was the question for our group to analyze the regeneration pattern of granulocytes in patients receiving a myeloablative therapy for the treatment of hemopoietic malignancies, and subsequently a transfusion of blood-derived cryopreserved stem cells after appropriate blood stem cell expansion protocols.…”