Results of cytomegalovirus DNA viral loads expressed in copies per millilitre and international units per millilitre are equivalent

Dimech, Wayne; Cabuang, Liza; Grunert, Hans‐Peter; Lindig, Vanessa; James, Vivienne L. A.; Sénéchal, Brigitte; Vincini, Giuseppe; Zeichhardt, Heinz

doi:10.1016/j.jviromet.2017.11.001

Cited by 8 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CMV testing was intensified for 6 months following prophylaxis or treatment of infection independent of the time in the transplant course. A CMV DNA viral load of ≥1000 copies/mL or ≥830 IU/mL was the threshold for commencement of treatment ( 17 – 19 ). The duration of CMV viremia was defined as the time in days from the first to the last positive CMV test for the episode.…”

Section: Methodsmentioning

confidence: 99%

Viral load kinetics and the clinical consequences of cytomegalovirus in kidney transplantation

Dobrer,

Sherwood,

Hirji

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

BackgroundDespite advances in clinical management, cytomegalovirus (CMV) infection remains a serious complication and an important cause of morbidity and mortality following kidney transplantation. Here, we explore the importance of viral load kinetics as predictors of risk and potential guides to therapy to reduce transplant failure in a large longitudinal Genome Canada Transplant Consortium (GCTC) kidney transplant cohort.MethodsWe examined the relationship between CMV infection rates and clinical characteristics, CMV viral load kinetics, and graft and patient outcomes in 2510 sequential kidney transplant recipients in the British Columbia Transplant Program. Transplants were performed between January 1, 2008, and December 31, 2018, were managed according to a standard protocol, and were followed until December 31, 2019, representing over 3.4 million days of care.ResultsLongitudinal CMV testing was performed in 2464 patients, of whom 434 (17.6%) developed a first episode of CMV viremia at a median of 120 (range: 9–3906) days post-transplant. Of these patients, 93 (21.4%) had CMV viremia only and 341 (78.6%) had CMV viremia with clinical complications, of whom 21 (4.8%) had resulting hospitalization. A total of 279 (11.3%) patients died and 177 (7.2%) patients lost their graft during the 12 years of follow-up. Patients with CMV infection were at significantly greater risk of graft loss (p=0.0041) and death (p=0.0056) than those without. Peak viral load ranged from 2.9 to 7.0 (median: 3.5) log10 IU/mL, the duration of viremia from 2 to 100 (15) days, and the viral load area under the curve from 9.4 to 579.8 (59.7) log10 IU/mL × days. All three parameters were closely inter-related and were significantly increased in patients with more severe clinical disease or with graft loss (p=0.001). Duration of the first CMV viremic episode greater than 15 days or a peak viral load ≥4.0 log10 IU/mL offered simple predictors of clinical risk with a 3-fold risk of transplant failure.ConclusionViral load kinetics are closely related to CMV severity and to graft loss following kidney transplantation and provide a simple index of risk which may be valuable in guiding trials and treatment to prevent transplant failure.

show abstract

Section: Methodsmentioning

confidence: 99%

Viral load kinetics and the clinical consequences of cytomegalovirus in kidney transplantation

Dobrer,

Sherwood,

Hirji

et al. 2024

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…28 Recent results indicate that VLs reported in copies/mL and IU/mL are comparable because of assay calibration on this standard, but the observed range of results was smaller, when reported in IU/mL. 36 Hence, HCMV DNA levels should be given as HCMV DNA IU/mL after standardisation.…”

Section: Human Cytomegalovirus Detection By Pcr Quantification Is Unablementioning

confidence: 98%

Comparison of quantitative real‐time PCR and short‐term (18‐hour) microculture in diagnosis of fetal cytomegalovirus infection: Impact of hyperimmunoglobulin treatment

Penka¹,

Kagan

Goelz

et al. 2018

Prenatal Diagnosis

View full text Add to dashboard Cite

show abstract

“…In a recently published EQA study, the variation in results reported in IU per milliliter was lower than that for results reported in copies per milliliter, demonstrating that the use of the CMV WHO IS improves the reproducibility and comparability of CMV viral load results across laboratories (58). Consequently, the recently revised international guidelines on the management of CMV in solid organ transplantation recommend that all results should be reported as IU per milliliter (59).…”

Section: Assay Harmonization Using Who Issmentioning

confidence: 99%

Standardization of Nucleic Acid Tests: the Approach of the World Health Organization

Baylis

Wallace²,

McCulloch³

et al. 2019

J Clin Microbiol

View full text Add to dashboard Cite

The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference materials have subsequently found utility in the diagnosis and monitoring of a wide range of infectious diseases in clinical microbiology laboratories worldwide. WHO collaborating centers develop ISs and coordinate international studies for their evaluation. The WHO Expert Committee on Biological Standardization is responsible for the endorsement of new standardization projects and the establishment of new and replacement ISs. Potencies of ISs are defined in international units (IU); the reporting in IU for assays calibrated with an IS (or secondary standards traceable to the IS) facilitates comparability of results for different assays and determination of assay parameters such as analytical sensitivities.

show abstract

Results of cytomegalovirus DNA viral loads expressed in copies per millilitre and international units per millilitre are equivalent

Cited by 8 publications

References 13 publications

Viral load kinetics and the clinical consequences of cytomegalovirus in kidney transplantation

Viral load kinetics and the clinical consequences of cytomegalovirus in kidney transplantation

Comparison of quantitative real‐time PCR and short‐term (18‐hour) microculture in diagnosis of fetal cytomegalovirus infection: Impact of hyperimmunoglobulin treatment

Standardization of Nucleic Acid Tests: the Approach of the World Health Organization

Contact Info

Product

Resources

About