Results of phytochemical screening in aerial organes of Bidens frondosa L.

Abstract

Introduction. The work presents the results of a comprehensive study of the grass of Bidens frondosa L. of the family Asteraceae, growing on the territory of the Krasnodar Territory and the Republic of Adygea. Objective: to carry out phytochemical screening of the main groups of biologically active substances and quantitative determination by methods of spectrophotometry and HPLC. Material and methods. The plant material is represented by dried air-shadow grass of the Bidens frondosa L. (September 2020 and 2022). Samples of herbarium collected by the authors are at the Department of Pharmacognosy, Botany and Technology of Phytotherapy of Pyatigorsk medical and pharmaceutical institute. Results. Complex studies have been carried out, including the determination of commodity quality indicators of aerial organs Bidens frondosa L. Determination of humidity in the raw material content is 9.9%. The definition of common ash is 7.3%. The ash content, insoluble in 10% hydrochloric acid solution, is 7%. In alcohol and aqueous extracts of herbs Bidens frondosa L. have been found: flavonoids, polysaccharides and tannins. UV spectrophotometry and HPLC were used to quantify the flavonoid sum content. The amount of flavonoids per routine in alcohol extraction was 3.13%. The herb B. frondosa contains gallic acid, chlorogenic acid, caffeic acid, rutin, luteolin-7-glycoside and quercetin. The substance whose content is greatest in the raw material is unknown. Presumably, this compound is Okanin from the group of chalcons. Probably, this compound has such a pronounced anti-allergic effect. Conclusion. The phytochemical analysis of the main groups of biologically active substances of the olic sequence of grass was conducted. During the work, the presence of flavonoids, polysaccharides and tannins was determined. The quantitative content of flavonoids, сhalcons and polysaccharides is determined.