Results of the first frozen bovine embryos exporied from the USA to Argentina

Munar, C.J.; Hasler, J.F.

doi:10.1016/0093-691x(89)90638-9

Cited by 4 publications

(1 citation statement)

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“…These animals were virgin Holstein heifers, 12 to for 4-6 days and selected to Serve as nuclear donors for ers were Ch'Xked for genera1 tally to check for were watched regularly for Signs of estrus in Order to ascertain which animals were and to Predict and Palpated Frozen embryos at late morula stage were kindly given by Em Tran, Inc. These were frozen by their standard procedures (Munar and Hasler, 1989). Fresh embryos were collected by nonsurgical flush of superovu-soundness.…”

Section: Methodsmentioning

confidence: 99%

Nuclear transfer in cattle: Effect of nuclear donor cells, cytoplast age, co‐culture, and embryo transfer

Yang

Jiang

Farrell

et al. 1993

Molecular Reproduction Devel

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There are many factors affecting the efficiency of nuclear transfer technology. Some are evaluated here using our novel approach by enucleating oocytes at 20-22 hr after in vitro maturation (IVM), culturing the enucleated oocytes (cytoplasts) for 8-10 hr or 18-20 hr to gain activation competence and then conducting nuclear transfer. In the first experiment, we demonstrated that cumulus cell (CC) monolayer can support some cloned embryos to develop into morulae or blastocysts. Co-culture with CC and bovine oviduct epithelial cell (BOEC) monolayers resulted in no differences (P > 0.05) in supporting the development of cloned embryos (Experiment 2). When in vitro matured oocytes were enucleated at 22 hr after IVM followed by nuclear transfer 18-20 hr later, cleavage and morula or blastocyst development of the cloned embryos were similar to those resulting from the enucleated oocytes which had been matured in vivo (Experiment 3). Frozen embryos as nuclear donor cells worked equally well as fresh embryos for cloning in embryo development which was superior to IVF embryos (Experiment 4). However, fresh embryos resulted in a higher proportion (P < 0.05) of blastomere recovery than did frozen of IVF embryos. Finally, embryo transfer of cloned embryos from our procedure produced a viable calf, demonstrating the commercial value of this novel approach of the technology.

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Section: Methodsmentioning

confidence: 99%