Results of the flow cytometry ACTG quality control program: Analysis and findings

Paxton, Helene; Kidd, Pamela; Landay, Alan; Giorgi, Janis V.; Flomenberg, Neal; Walker, Edward T.; Valentine, Fred; Fahey, John L.; Gelman, Rebecca

doi:10.1016/0090-1229(89)90194-3

Cited by 67 publications

(45 citation statements)

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“…Flow cytometric analysis and HIV culture were performed at each site. All laboratories participated in qualityassurance programs of the AIDS Clinical Trials Group [30].…”

Section: Methodsmentioning

confidence: 99%

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Mekmullica¹,

Brouwers²,

Charurat³

et al. 2009

CLIN INFECT DIS

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“…Flow cytometric analysis and HIV culture were performed at each site. All laboratories participated in qualityassurance programs of the AIDS Clinical Trials Group [30].…”

Section: Methodsmentioning

confidence: 99%

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Mekmullica¹,

Brouwers²,

Charurat³

et al. 2009

CLIN INFECT DIS

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“…The need for quality assurance of flow cytometric data has been emphasized (8,15,23), and several quality assurance testing programs have been described (6,25,26,29,33). These programs measure precision and accuracy.…”

mentioning

confidence: 99%

Evaluation of a dual‐color flow cytometry immunophenotyping panel in a multicenter quality assurance program

Schenker¹,

Hultin²,

Bauer

et al. 1993

Cytometry

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A basic immunophenotyping panel that employed dual-color combinations of fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated monoclonal antibodies (mAb; FITC-CD45/PE-CD14, FITC-IgGI/PE-IgGz, FITC-CD3/PE-CDS, FITC-CD3/PE-CD4, FITC-CD3/ PE-CD16 + PE-CD56, and PE-CD19) was utilized in a quality assurance program to determine whether the 4 laboratories participating in a multicenter AIDS study obtained similar lymphocyte subset percentage values for T cells, B cells, NK cells, and CD4+ and CDS+ T cells. Over a 11/2 year period, 78 shared peripheral blood specimens were prepared and analyzed in each laboratory. The C D~E I~' '~~~C D~~-percentage for each specimen was used to correct that individual's lymphocyte subset values. Interlaboratory coefficients of variation (CV) for the human immunodeficiency virus type I (HIV) seronegative (n = 38) and HIV-seropositive (n = 40) specimens using this panel were <3% for total T cells; 4% for CD4+ T cells and CD8+ T cells; 517% for B and NK cells; and 4%for CD4T/CDST ratios. The 6-tube basic immunophenotyping panel has several notable features: a) for clinical studies, it permits comprehensive evaluation of an individual's major lymphocyte subsets, i.e., T, B, NK, and CD4+ and CDS+ T cells; b) for interlaboratory proficiency testing programs, it allows the detection of differences among laboratories in measurements of several functionally distinct cell populations; and c) for within-sample quality assurance, it provides several quality control checks, including the lymphosum, i.e., the sum of an individual's corrected T + B + NK values, a sum that was generally 100 2 5% on the HIV-seronegative specimens analyzed in this study. o 1993 Wiley-Liss, Inc.

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“…É importante e ideal que as subpopulações linfocitárias T CD4 e CD8 sejam preservadas, enquanto outros componentes que possam interferir na identificação desses linfócitos sejam eliminados. Desta forma, o método recomendado para preparação das amostras, segundo as diretrizes do National Institute of Allergy and Infectious Diseases, é a utilização da lise do sangue total, visto que os métodos de separação de células linfocitárias por diferença de gradiente de densidade pelo Ficoll ou Histopaque requerem maior volume de sangue e as alterações nas subpopulações linfocitárias são maiores (PAXTON et al, 1989). BYRNE et al (2000) observaram que o isolamento de leucócitos do sangue periférico pelo método de lise do sangue total produziu fragmentos, dificultando a padronização.…”

Section: Preparação Da Amostraunclassified

Metodologia e aplicação da citometria de fluxo na hematologia veterinária

et al. 2005

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Results of the flow cytometry ACTG quality control program: Analysis and findings

Cited by 67 publications

References 0 publications

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Early Immunological Predictors of Neurodevelopmental Outcomes in HIV‐Infected Children

Evaluation of a dual‐color flow cytometry immunophenotyping panel in a multicenter quality assurance program

Metodologia e aplicação da citometria de fluxo na hematologia veterinária

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