Results of the systematic survey and control of Plum pox potyvirus in Slovenia

Abstract

Sharka, caused by Plum pox potyvirus (PPV), was first observed in Slovenia in 1987. In the following years, the presence of PPV was confirmed by DAS‐ELISA in samples originating from orchards, individual trees and propagation material of stone fruits. In 1998, a systematic survey was initiated to prevent and control the spread of sharka and to establish pest‐free production sites. In the first 3 years of the systematic survey, the presence of PPV was also monitored outside production sites for propagating material and their buffer zones, in order to establish the current incidence of sharka in Slovenia. The results showed an overall presence of PPV infection in Slovenia. Since 2000, the survey has focused mainly on nurseries, mother trees and stool‐beds. In spite of strict official supervision and eradication measures, the incidence of sharka in propagating material production sites increased until 2002. A significant decrease in the number of PPV‐infected mother trees, stool‐beds, nurseries and buffer zones was finally obtained in 2002, as a consequence of the vast effort dedicated to containment of sharka in Slovenia.

Total RNA extraction method and Prunus species influence the detection of Plum pox potyvirus by real-time RT-PCR

“…Due to the high incidence of PPV infection a systematic survey of PPV was initiated in 1998 to prevent and control the spread of sharka. Since 2000 the survey has focused mainly on nurseries, mother trees and stool-beds (Viršček Marn et al, 2004).…”