Resumption of meiotic maturation of oocytes, pre- and post-implantation mortality of embryos under ten-time intravenous treatment of silver nanoparticles in mice

Abstract

Background: In recent years the use of silver nanoparticles (AgNP) has increased significantly being focused on assessing human health and environmental risks of nanotechnology.Methods: Research (two series) has been done on white laboratory mice. One dose (20 mg/kg) has been investigated. Frequency of treatment: one time per day for 10 times. Material for the study (ovaries, tubes and uterus) was taken under anesthetic anesthesia on the 10/11th, 21/22nd, 33/34th, 42/43rd days (after the last treatment). Oocyte meiotic maturation, pre- and post-implantation mortality of embryos under ten-time intravenous treatment of AgNP were investigation.Results: Under the ten-time treatment of AgNPs, the inhibition of reproductive function in female mice occurs: a decrease in the number and quality of ovarian oocytes; after male mice were planted, the females do not get pregnant to 21/22 day. The reproductive function in experimental animals is restored on the 40th day after the last treatment with AgNPs; there is no differences between the values of pre- and post-implantation mortality of embryos on the 33/34th day after male mice were planted; in one of the three experimental animals, 7 live pups were born on the 42/43rd day after the male was planted (in animals of the control group during this period: twice (6 ± 1 (n = 6) live pups).Conclusions: In mice females, under a ten-time treatment of AgNPs, the inhibition of reproductive function takes place; with the termination of the AgNPs treatment, the reproductive function is restored.

Effect of one-time dextran-polyacrylamide polymer matrixes treatment on female reproductive function

“…7 Also we received data that under conditions of ten-time administration of AgNPs (20 mg kg), the inhibition of reproductive function in female females is observed: a decrease in the number and quality oocytes was established, but the reproductive function in experimental animals was restored 37 days after the last administration of AgNPs and there were no differences between the values of pre-and post-implantation mortality of embryos on the 33/34 th day after male planting. 8 Since authors had previously used a dose of AgNPs (2 mg/kg), this time again we applied the same 2 mg/kg and 10 times smaller dose 0.20 mg/kg of AgNPs that were There is evidence that AgNPs influence the induction of oxidative damage, change the regulation of enzymes, which are responsible for removing free radicals, change regulation of genes expression, which are involved in apoptosis and disregulation of cellular structures involved in storage, detoxification and metabolism of metals in various organs. 9,10 Silver ions were equally toxic as both metal particles containing 80% silver and pure AgNP indicating that at least their toxic potential was similar.…”