Resumption of oocyte meiosis in mammals: On models, meiosis activating sterols, steroids and EGF-like factors

Tsafriri, A.; Cao, Xiumei; Ashkenazi, H.; Motola, S.; Popliker, Malka; Pomerantz, Seymour H.

doi:10.1016/j.mce.2004.09.009

Cited by 51 publications

(36 citation statements)

References 55 publications

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“…Exogenous EGF could mimic some effects of the EGF-like peptides expressed by follicular somatic cells following induction of the preovulatory surge of LH. Indeed, amphiregulin, epiregulin, and b-cellulin (all members of the EGF family) have been shown to mediate, at least partially, the LH stimulation of oocyte maturation and cumulus expansion (Park et al 2004, Tsafriri et al 2005, Downs & Chen 2008. A recent study performed in mice by Shimada et al (2006) suggested that amphiregulin-dependent pathways regulated genes not only involved in the induction of cumulus expansion such as PTGS2 but also involved in steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Nuttinck¹,

Guienne²,

Clément³

et al. 2008

Reproduction

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Prostaglandin E 2 (PGE 2 ) and progesterone appear to be critical mediators of cumulus expansion and the resumption of oocyte meiosis. The aim of this study was to identify the types of prostaglandin E synthase (PTGES) expressed in the bovine cumulus-oocyte complex (COC), to characterize their temporal expression during the periconceptional interval using an in vitro model of maturation (IVM) and fertilization (IVF), and to compare their expression with the level of steroidogenic gene expression. Real-time RT-PCR analysis revealed that enzymes related to the PGE 2 biosynthesis pathway were mainly expressed during IVM. Transcripts encoding PTGES1-3 were detected in bovine COCs. Only the expression of PTGES1 significantly increased during IVM whereas that of PTGES2 and PTGES3 remained unchanged. The induction of PTGES1 expression paralleled the induction of prostaglandin G/H synthase-2 (PTGS2) expression and the amounts of PGE 2 secreted by maturing COCs. Concomitantly, cholesterol side chain cleavage cytochrome P450 expression was significantly upregulated in maturing COCs and the high level of expression persisted in fertilized COCs. The expression of the StAR protein remained constant during IVM and then decreased significantly during IVF. Expression of the progesterone catabolic-related enzyme, 20a-hydroxysteroid dehydrogenase significantly decreased throughout the periconceptional interval. This was associated with a rising level of progesterone released by COCs in the culture media. In conclusion, our results suggest that the periconceptional differentiation of the bovine COC includes the transient induction of PGE 2 biosynthetic activity via the PTGS2/PTGES1 pathway during the maturation period and the increasing ability to produce progesterone from the immature to the fertilized stages. Reproduction (2008) 135 593-603

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Section: Discussionmentioning

confidence: 99%

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Nuttinck¹,

Guienne²,

Clément³

et al. 2008

Reproduction

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“…However, there is evidence that it improves the ability of oocytes to complete meiosis to metaphase II, as well as the ability of fertilized oocytes to develop to the two-cell and blastocyst stages (Hegele-Hartung et al 1999, Cukurcam et al 2003, Griffin et al 2004, Marin Bivens et al 2004a, 2004b. For further discussion, see Byskov et al (2002) and Tsafriri et al (2002Tsafriri et al ( , 2005.…”

Section: How Does Lh Trigger Meiotic Resumption?mentioning

confidence: 99%

Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation

Mehlmann¹

2005

Reproduction

437

307

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Mammalian oocytes grow and undergo meiosis within ovarian follicles. Oocytes are arrested at the first meiotic prophase, held in meiotic arrest by the surrounding follicle cells until a surge of LH from the pituitary stimulates the immature oocyte to resume meiosis. Meiotic arrest depends on a high level of cAMP within the oocyte. This cAMP is generated by the oocyte, through the stimulation of the G s G-protein by the G-protein-coupled receptor, GPR3. Stimulation of meiotic maturation by LH occurs via its action on the surrounding somatic cells rather than on the oocyte itself. LH induces the expression of epidermal growth factor-like proteins in the mural granulosa cells that act on the cumulus cells to trigger oocyte maturation. The signaling pathway between the cumulus cells and the oocyte, however, remains unknown. This review focuses on recent studies highlighting the importance of the oocyte in producing cAMP to maintain arrest, and discusses possible targets at the level of the oocyte on which LH could act to stimulate meiotic resumption.

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“…EGF activates the genes involved in cumulus expansion and follicle rupture in vitro and induces the release of the mature ovum in vivo [52]. During hCG/EGF treatment, oocytes spontaneously dissociate from their follicular cells, which then gradually degenerate (Fig.…”

Section: Growth Of Preantral Folliclesmentioning

confidence: 99%

Derivation of Histocompatible Stem Cells from Ovarian Tissue

2010

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Abstract. Somatic cell nuclear transfer, the first established technique for producing patient-specific autologous stem cells, inevitably requires the sacrifice of viable embryos. To circumvent the serious ethical issues associated with this use of embryos, researchers have developed several alternative methods for the production of histocompatible stem cells. In our research, we have used two methods to derive histocompatible stem cells from murine ovarian tissue. First, we have established autologous stem cells by culturing degeneration-fated preantral follicles to produce developmentally competent, mature oocytes and then parthenogenetically activating these mature oocytes to acquire genetic homogeneity. Second, we have used cell-to-cell interactions to derive stem cells from ovarian stromal cells without undertaking genetic modification. We have successfully derived autologous murine stem cells by manipulating primary and early secondary follicles in vitro, and this method has proved successful even for follicles retrieved from aged ovaries. Furthermore, we believe that it will be possible to isolate stem cells directly from nongermline ovarian tissue or to derive stem cells by culturing the ovarian cells with other somatic cells. If achieved, these aims will greatly advance the development of induced pluripotent stem cell technology, as well as tissue-specific stem cell research. In this review, we introduce the relevant technologies for establishing histocompatible stem cells from ovarian tissue cells without undertaking genetic manipulation and review the current limitations of, and future research directions in, stem cell biology. Key words: Autologous, Ovarian cells, Parthenogenesis, Preantral follicle, Stem cell (J. Reprod. Dev. 56: [481][482][483][484][485][486][487][488][489][490][491][492][493][494] 2010) luripotent stem cells are widely recognized as critical to the success of certain cutting-edge medical technologies, such as cell replacement, organ regeneration, tissue engineering, and rejuvenation therapy [1][2][3]. However, the potential clinical practicability of stem-cell-based techniques is limited by the absolute requirement for patient-stem cell histocompatibility or patient acquisition of immune tolerance [4]. Another obstacle to the use of stem cells is the current lack of effective techniques for regulating cell-to-cell interactions and for inducing target differentiation of stem cells. The future success of tissue regeneration therapies will also depend on the optimization of stem-cell transplantation techniques and the development of strategies for inducing transplanted stem cells to resume their normal functions. In addition, all stem cell-based procedures must be carried out in an ethically acceptable manner.Efforts to develop stem-cell-based techniques such as those described above have been extensive. In our research, we have consistently attempted to develop novel methodologies for the establishment of histocompatible stem cells. At a very early stage in stem cell research, a ...

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Resumption of oocyte meiosis in mammals: On models, meiosis activating sterols, steroids and EGF-like factors

Cited by 51 publications

References 55 publications

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation

Derivation of Histocompatible Stem Cells from Ovarian Tissue

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