Resuscitation and intensive care in acute massive blood loss in adults (clinical guidelines)

Григорьев, Е. В.; Лебединский, К.М.; Щеголев, А. В.; Бобовник, С. В.; Буланов, А. Ю.; Заболотских, И. Б.; Синьков, С. В.; Shen, Ningyan; Корнелюк, Р. А.

doi:10.17116/anaesthesiology20200115

Cited by 11 publications

(1 citation statement)

References 134 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Так, динамика показателей буферных систем свидетельствовала о менее выраженном расходовании гидрокарбоната крови после введения раствора Р-1. После вливания растворов Р-2 и «Гемостабил» содержание HCO 3 продолжало медленно снижаться на фоне уменьшения рН, что говорит о продолжении развития метаболического ацидоза [18]. При этом парциальное давление кислорода в крови у животных после инфузии раствора Р-1 было статистически значимо более высоким по сравнению с аналогичными показателями у животных в группе 4.…”

Section: Discussionunclassified

Pre-clinical evaluation of effectiveness of infusion solutions with different osmolarity during acute blood loss in experiments on rats

Shperling,

Krupin,

Arokina

et al. 2024

Sibirskij nauchnyj medicinskij zhurnal

View full text Add to dashboard Cite

The aim of the research was to experimentally evaluate the effectiveness of infusion solutions with different osmolarity during acute blood loss in experiments on rats. Material and methods. Experiments approved by the local Ethics Committee, have been done on 30 male rats line Vistar (320 ± 35 g). Design of the research included anesthesia, simulation of acute blood loss to 50 % of circulating blood volume, its replenishment with experimental infusion solutions based on polyglycan derivatives (estimated osmolarity 1026.6, 1784.2, 2566.6 mOsm/l), research of survivability, functional state of cardiovascular and respiratory systems, blood acid-base status and gas partial pressure 5, 30, 60, 120, 180 min after the beginning of replenishment of circulating blood volume. The infusion solution Hemostabil was used as a comparison product. Results. The highest survival rate, restoration of hemodynamics and acid-base state and blood acid-base status and gas partial pressure were obtained as a result of infusion of solution with osmolarity 1026.6 mOsm/l. In this group of animals survivability accounted for 100 %, arterial pressure restored in 5 minutes after the start of infusion, indicators of blood acid-base status and gas partial pressure normalized in 120 min of experiment. All of the results had statistically significant differences (p < 0.05) relative to values in other experimental groups. Conclusions. Sample of solution with osmolarity 1026.6 mOsm/l is the most effective in restoration of hemodynamics of rats with the blood loss up to 50 % of circulating blood volume. The expediency of the researches of safety of this infusion solution based on polyglucan derivatives in the capacity of medication for replenishment acute blood loss is substantiated.

show abstract

Section: Discussionunclassified

Pre-clinical evaluation of effectiveness of infusion solutions with different osmolarity during acute blood loss in experiments on rats

Shperling,

Krupin,

Arokina

et al. 2024

Sibirskij nauchnyj medicinskij zhurnal

View full text Add to dashboard Cite

show abstract

Effect of Systemic Hypothermia on Reactions of Pial Arteries to Acute Massive Blood Loss in Anesthetized Rats

Мельникова

2022

J Evol Biochem Phys

View full text Add to dashboard Cite

Experimental model of acute blood loss using rats for screening evalution of non-specific activity of infusion solutions

Shperling¹,

Krupin²,

Arokina³

et al. 2023

Medical academic journal

View full text Add to dashboard Cite

BACKGROUND: In accordance with the current regulations, preclinical evaluation of the effectiveness of plasma substitutes for acute blood loss is carried out mainly on large laboratory animals (dogs, pigs) using a wide range of methods for assessing the structural and functional state of organs and systems of a biological object. It requires large expenditure of material resources and time, which is impractical at the stage of screening the effectiveness of newly developed infusion agents. In this regard, an urgent task is to develop a standardized model of acute blood loss on small laboratory animals for screening evaluation of the specific activity of infusion solutions with a subsequent research involving large laboratory animals. It is advisable to use laboratory rats as a biological object as they are the most suitable of the group of small laboratory animals for similarity of physiological laboratory indicators with humans. AIM: To develop a model of acute blood loss using small laboratory animals for screening evaluation of specific infusion solution activity. MATERIALS AND METHODS: Experiments were carried out on rats of the Vistar line with a weight of 330 41 g. The animals were divided into 3 groups: 1 experimental (20 individuals with acute blood loss simulation without treatment), 2 experimental (20 individuals with acute blood loss simulation and its replacement with Rheopolyglucin), intact (10 individuals without modeling of blood loss). The study design included: general anesthesia (intramuscular injection Zoletil 100 and Xylazin 2% in a ratio of 1 : 5 at the rate of 0.01 ml/kg of weight), catheterization of the femoral artery followed by controlled hardware exfusion of blood at a rate of 0.5 ml/min until the establishment of persistent (for 2 minutes) arterial hypotension; hardware synchronous monitoring of mean arterial pressure (MAP) (by direct tonometry through the contralateral femoral artery); calculation of the percentage of blood loss from the estimated circulating blood volume (CBV) equal to 5% of the animals weight; heart rate (HR) (by electrocardiogram) during the first three hours after blood exfusion. In its capacity as a test drug Rheopolyglucin, which was administered through an arterial femoral catheter immediately after blood exfusion in volume and speed, equal to volume and speed of exfusion, was used. Additionally, for a comprehensive assessment of the mechanisms of maintaining hemodynamic parameters individual dynamic calculated indicators for each individual are proposed: the reduced shock volume of blood and the infusion efficiency indicator. RESULTS: All rats in the experimental group died, 25% of which 1720 minutes after blood exfusion, 75% in range from 45 to 90 minutes. Rheopoliglyukin infusion reduced the death of animals by up to 35% and delayed the average death time to 4555 minutes. A single exfusion of blood in rats resulted in loss of 79 ml of blood (4651% of circulating blood volume), which was accompanied by a decrease in mean arterial pressure and heart rate. Compensation for the decrease in circulating blood volume, including due to infusion, was manifested by an increase in these indicators. A sign of inefficiency of compensation was a slight increase of mean arterial pressure with dynamically increasing heart rate. It is proved that an increase in the values of calculated indicators (the given stroke volume of blood and the infusion efficiency indicator) are benchmarks for effective compensation of hemodynamic disorders, including as a result of infusion of hemodynamic drugs. CONCLUSIONS: The acute blood loss model with the calculation of the reduced shock volume of blood and the infusion efficiency index is advisable to use to assess the specific activity of infusion solutions in acute blood loss.

show abstract

Resuscitation and intensive care in acute massive blood loss in adults (clinical guidelines)

Cited by 11 publications

References 134 publications

Pre-clinical evaluation of effectiveness of infusion solutions with different osmolarity during acute blood loss in experiments on rats

Pre-clinical evaluation of effectiveness of infusion solutions with different osmolarity during acute blood loss in experiments on rats

Effect of Systemic Hypothermia on Reactions of Pial Arteries to Acute Massive Blood Loss in Anesthetized Rats

Experimental model of acute blood loss using rats for screening evalution of non-specific activity of infusion solutions

Contact Info

Product

Resources

About