Resuscitation of eleven-year VBNC Citrobacter

Dhiaf, Amel; Bakhrouf, Amina; Witzel, Karl-Paul

doi:10.2166/wh.2008.131

Cited by 18 publications

(12 citation statements)

References 5 publications

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“…This resuscitation has been strongly debated [21], [38], [39], as some authors suggest that the recovery of culturability is due to the presence and sudden growth of a few residual cells with a normal metabolism in a population predominantly non culturable. However, the recovery of cell division in a population of VBNC cells was described unambiguously for several bacteria [40], [41]. Cell resuscitation has been clearly demonstrated in vitro, in vivo and in situ [11].…”

Section: Resultsmentioning

confidence: 99%

Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

et al. 2013

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The Viable But Non Culturable (VBNC) state has been thoroughly studied in bacteria. In contrast, it has received much less attention in other microorganisms. However, it has been suggested that various yeast species occurring in wine may enter in VBNC following sulfite stress.In order to provide conclusive evidences for the existence of a VBNC state in yeast, the ability of Saccharomyces cerevisiae to enter into a VBNC state by applying sulfite stress was investigated. Viable populations were monitored by flow cytometry while culturable populations were followed by plating on culture medium. Twenty-four hours after the application of the stress, the comparison between the culturable population and the viable population demonstrated the presence of viable cells that were non culturable. In addition, removal of the stress by increasing the pH of the medium at different time intervals into the VBNC state allowed the VBNC S. cerevisiae cells to “resuscitate”. The similarity between the cell cycle profiles of VBNC cells and cells exiting the VBNC state together with the generation rate of cells exiting VBNC state demonstrated the absence of cellular multiplication during the exit from the VBNC state. This provides evidence of a true VBNC state. To get further insight into the molecular mechanism pertaining to the VBNC state, we studied the involvement of the SSU1 gene, encoding a sulfite pump in S. cerevisiae. The physiological behavior of wild-type S. cerevisiae was compared to those of a recombinant strain overexpressing SSU1 and null Δssu1 mutant. Our results demonstrated that the SSU1 gene is only implicated in the first stages of sulfite resistance but not per se in the VBNC phenotype. Our study clearly demonstrated the existence of an SO2-induced VBNC state in S. cerevisiae and that the stress removal allows the “resuscitation” of VBNC cells during the VBNC state.

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Section: Resultsmentioning

confidence: 99%

Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

et al. 2013

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“…They are found in different kinds of water bodies, including seawater (Maalej et al, 2004; Dhiaf et al, 2008), estuarine water (Oliver et al, 1995), stream water (Lemke and Leff, 2006), lake water (Signoretto et al, 2004), ground water (Cook and Bolster, 2007), tap water (Pawlowski et al, 2011) and drinking water (Byrd et al, 1991). Moreover, VBNC cells of Salmonella typhimurium were found in soil (Reissbrodt et al, 2002) and those of E. coli were found in processed food (Makino et al, 2000).…”

Section: Bacteria With a Vbnc Statementioning

confidence: 99%

“…Mukamolova et al (1998b) found that 3-month-old and 6-month-old VBNC cells of Micrococcus luteus can be resuscitated within two months and 10 days, respectively, suggesting that the resuscitation window of this species is around six months. According to the available information, there is great variation in the resuscitation windows between different species (Table 1), ranging from 4 days in S. enteritidis (Roszak et al, 1984) to 11 years in Citrobacter freundii (Dhiaf et al, 2008). …”

Section: Resuscitation Of Vbnc Cellsmentioning

confidence: 99%

The importance of the viable but non-culturable state in human bacterial pathogens

et al. 2014

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Many bacterial species have been found to exist in a viable but non-culturable (VBNC) state since its discovery in 1982. VBNC cells are characterized by a loss of culturability on routine agar, which impairs their detection by conventional plate count techniques. This leads to an underestimation of total viable cells in environmental or clinical samples, and thus poses a risk to public health. In this review, we present recent findings on the VBNC state of human bacterial pathogens. The characteristics of VBNC cells, including the similarities and differences to viable, culturable cells and dead cells, and different detection methods are discussed. Exposure to various stresses can induce the VBNC state, and VBNC cells may be resuscitated back to culturable cells under suitable stimuli. The conditions that trigger the induction of the VBNC state and resuscitation from it are summarized and the mechanisms underlying these two processes are discussed. Last but not least, the significance of VBNC cells and their potential influence on human health are also reviewed.

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“…3A). It is known that small colony variants (SCV) of bacteria can enter a viable but nonculturable (VBNC) state as a response to the environmental stresses involving nutrient, temperature, and osmotic conditions changes [Dhiaf et al 2008, Proctor et al 2006]. In order to examine if the “live” bacteria in low-power discharge argon treated biofilms were indeed VBNC bacteria, we fed discharge argon treated biofilms grown in 6-well plates with TSGB medium containing high concentration (10%) of glucose.…”

Section: Resultsmentioning

confidence: 99%

Insights into discharge argon-mediated biofilm inactivation

et al. 2013

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Formation of bacterial biofilms at solid-liquid interfaces creates numerous problems in biomedical sciences. Conventional sterilization and decontamination methods are not suitable for new and more sophisticated biomaterials. In this paper, the efficiency and effectiveness of gas discharges in inactivation and removal of biofilms on biomaterials were studied. We found that although discharge oxygen, nitrogen and argon all demonstrated excellent antibacterial and antibiofilm activity, gases with distinct chemical/physical properties underwent different mechanisms of action. Discharge oxygen and nitrogen mediated decontamination was associated with strong etching effects, which can cause live bacteria relocation and thus contamination spreading. On the contrary, although discharge argon at low powers maintained excellent antibacterial ability, it had negligible etching effects. Based on these results, an effective decontamination approach using discharge argon was established in which bacteria and biofilms were killed in situ and then removed from contaminated biomaterials. This novel procedure is applicable for a wide range of biomaterials and biomedical devices in an in vivo and clinical setting.

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Resuscitation of eleven-year VBNC Citrobacter

Cited by 18 publications

References 5 publications

Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

Characterization of the Viable but Nonculturable (VBNC) State in Saccharomyces cerevisiae

The importance of the viable but non-culturable state in human bacterial pathogens

Insights into discharge argon-mediated biofilm inactivation

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