Resuscitation of
            <i>vibrios</i>
            From the Viable But Nonculturable State is Induced By Quorum‐sensing Molecules

Ayrapetyan, Mesrop; Williams, Tiffany C.; Oliver, James D.

doi:10.1002/9781119004813.ch130

Cited by 4 publications

(3 citation statements)

References 42 publications

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“…VBNC cells are defined as microbes that fail to cultivate on routine media on which they usually grow and yet are still alive, a phenomenon that has been first described by Xu et al in 1982 for Vibrio ( V. ) cholerae [ 12 , 35 , 36 ]. Thus, the VBNC state could either be described as a “failure of the culture dependent technique” (as in hindering the bacterial growth), or as a dormant state in which the proliferation of the microbes is blocked until they get a “wake up call” [ 19 , 37 ]. As is so often the case when using such broad terminology as dead, alive, or dormant, it is not easy to draw simple singular conclusions.…”

Section: Current Explanations Of Antimicrobial Resistance Of Bactementioning

confidence: 99%

“…However, flow cytometry in contrast to fluorescence microscopy, is the more accurate method to estimate bacterial counts down to 2.5% live and 20% dead cells [25,81]. Nevertheless, viable staining methods such as the BacLight ™ allows the evaluation of the physiological state of individual bacteria regardless of their species, growth conditions, and treatments including antibiotics [16,19,67]. Using direct fluorescence staining microscopy in combination with the BacLight ™ kit, Chaiyanan et al [68] successfully monitored Bamford et al [31] monitored E. coli cells in the VBNC state via a single-cell approach combined with the BacLight™ staining before, during, and after the ampicillin treatment.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

“…The VBNC state is a condition of reduced metabolic activity, induced in response to conditions of stress. Entry into the VBNC state can be facilitated by a number of environmental factors, such as a lack of nutrients, temperature shifts, or the presence of antibiotics, disinfectants, and sanitizers [ 12 , 17 , 18 , 19 ]. When a microorganism enters the VBNC state, both the morphology and physiology of the cell changes to allow its survival under adverse conditions.…”

Section: Introductionmentioning

confidence: 99%

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How to Evaluate Non-Growing Cells—Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria

et al. 2021

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Thanks to the achievements in sanitation, hygiene practices, and antibiotics, we have considerably improved in our ongoing battle against pathogenic bacteria. However, with our increasing knowledge about the complex bacterial lifestyles and cycles and their plethora of defense mechanisms, it is clear that the fight is far from over. One of these resistance mechanisms that has received increasing attention is the ability to enter a dormancy state termed viable but non-culturable (VBNC). Bacteria that enter the VBNC state, either through unfavorable environmental conditions or through potentially lethal stress, lose their ability to grow on standard enrichment media, but show a drastically increased tolerance against antimicrobials including antibiotics. The inability to utilize traditional culture-based methods represents a considerable experimental hurdle to investigate their increased antimicrobial resistance and impedes the development and evaluation of effective treatments or interventions against bacteria in the VBNC state. Although experimental approaches were developed to detect and quantify VBNCs, only a few have been utilized for antimicrobial resistance screening and this review aims to provide an overview of possible methodological approaches.

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Section: Current Explanations Of Antimicrobial Resistance Of Bactementioning

confidence: 99%

Section: Fluorescence Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How to Evaluate Non-Growing Cells—Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria

et al. 2021

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Assessment of bacterial viability: a comprehensive review on recent advances and challenges

2019

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Long-Term Colonization Dynamics of ProbioticAliivibriospp. in Atlantic Salmon (Salmo salar) Following Bath Administration

Steen Dobloug,

Iakhno,

Foyn Nørstebø

et al. 2024

Preprint

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Ulcerative conditions present a major challenge in Norwegian salmon farming. Probiotic Aliivibrio species have previously been demonstrated to provide health benefits in both Atlantic salmon and lumpfish, although the underlying mechanisms and the host-bacteria interactions remain unclear. This study aimed to investigate whether these bacteria could colonize Atlantic salmon following bath administration, determine the tissue tropism, and assess the duration of colonization. We examined the host microbiota using culture-based methods, qPCR and immunohistochemistry techniques specifically designed to target the applied Aliivibrio strains. Our findings reveal that the probiotic bacteria can successfully colonize Atlantic salmon and persist for at least nine months post-administration. We identified the administered strains in the skin and underlying tissue with all three methods. The probiotics were also identified in the distal intestine and the visceral organs. Additionally, we isolated the probiotic Aliivibrio species from mixed cultures in ulcerated areas. While viable bacteria were recoverable from recently euthanized fish, tissue decay promoted bacterial recovery of the administered species across all experiments. Given prior evidence on ulcer reduction associated with these probiotics, competitive exclusion appears to be a plausible mechanism of action, though further investigation is warranted.

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Resuscitation of vibrios From the Viable But Nonculturable State is Induced By Quorum‐sensing Molecules

Cited by 4 publications

References 42 publications

How to Evaluate Non-Growing Cells—Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria

How to Evaluate Non-Growing Cells—Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria

Assessment of bacterial viability: a comprehensive review on recent advances and challenges

Long-Term Colonization Dynamics of ProbioticAliivibriospp. in Atlantic Salmon (Salmo salar) Following Bath Administration

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