Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Su, Xiaomei; Zhang, Shuo; Mei, Rongwu; Zhang, Yu; Hashmi, Muhammad Zaffar; Liu, Jingjing; Lin, Hongjun; Ding, Li; Sun, Fenglian

doi:10.1111/1751-7915.13256

Cited by 36 publications

(15 citation statements)

References 43 publications

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“…One potential resuscitation mechanism of Rpf is remodeling of the peptidoglycan in the cell wall of dormant cells, generating muropeptides that may serve as a 'wake-up call', stimulating the growth of dormant cells (Kana and Mizrahi 2010). Su et al (2018) enhanced the cellulose-degrading capability of the bacterial community in composting by using the Rpf protein to resuscitate viable but nonculturable bacteria (Table 1). In gram-negative bacteria, gene expression regulator autoinducers (AIs), with the ability to stimulate growth rates, have also been discovered (Freestone et al 1999).…”

Section: For Dormant Bacteriamentioning

confidence: 99%

Strategies for culturing active/dormant marine microbes

Ouyang

Chen

et al. 2020

Mar Life Sci Technol

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Microorganisms are ubiquitous in the ocean environment and they play key roles in marine ecosystem function and service. However, many of their functions and phenotypes remain unknown because indigenous marine bacteria are mostly difficult to culture. Although many novel techniques have brought previously uncultured microbes into laboratory culture, there are still many most-wanted or key players that need to be cultured from marine environments. This review discusses possible reasons for 'unculturable microbes' and categorizes uncultured bacteria into three groups: dominant active bacteria, rare active bacteria, and dormant bacteria. This review also summarizes advances in cultivation techniques for culturing each group of unculturable bacteria. Simulating the natural environment is an effective strategy for isolating dominant active bacteria, whereas culturomics and enrichment culture methods are proposed for isolating rare active bacteria. For dormant bacteria, resuscitation culture is an appropriate strategy. Furthermore, the review provides a list of the most-wanted bacteria and proposes potential strategies for culturing these bacteria in marine environments. The review provides new insight into the development of strategies for the cultivation of specific groups of uncultured bacteria and therefore paves the way for the detection of novel microbes and their functions in marine ecosystems.

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Section: For Dormant Bacteriamentioning

confidence: 99%

Strategies for culturing active/dormant marine microbes

Ouyang

Chen

et al. 2020

Mar Life Sci Technol

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“…Filter paper cellulase (FPCase) and carboxymethyl-cellulase (CMCase) were detected in both pure and mixed cultures. Cellulase activities were higher in both mixed and Rpf-supplemented cultures than in the pure and Rpf-lacking ones, respectively (Su et al 2018b). Su and colleagues resuscitated functional strains from a highly nitrogen-polluted river sediment that reached ammonium and nitrate removal rates of 2.23 and 0.86 mg L -1 h -1 , respectively.…”

Section: Potential Environmental Functions Of Vbnc Bacteriamentioning

confidence: 98%

Challenges of unculturable bacteria: environmental perspectives

Bodor

Bounedjoum

Vincze

et al. 2020

Rev Environ Sci Biotechnol

241

136

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Environmental biotechnology offers several promising techniques for the rehabilitation of polluted environments. The modern industrialized world presents novel challenges to the environmental sciences, requiring a constant development and deepening of knowledge to enable the characterization of novel pollutants and a better understanding of the bioremediation strategies as well as their limiting factors. The success of bioremediation depends heavily on the survival and activities of indigenous microbial communities and their interaction with introduced microorganisms. The majority of natural microbiomes remain uncultivated; therefore, further investigations focusing on their intrinsic functions in ecosystems are needed. In this review, we aimed to provide (a) a comprehensive overview of the presence of viable but nonculturable bacteria and yet-to-becultivated cells in nature and their diverse awakening strategies in response to, among other factors, signalling extracellular metabolites (autoinducers, resuscitation promoting factors, and siderophores); (b) an outline of the trends in isolating unculturable bacteria; and (c) the potential applications of these hidden players in rehabilitation processes. Keywords Uncultured bacteria Á Viable but nonculturable bacteria Á Bacterial resuscitation Á Environmental application of VBNC bacteria Á Exploitation of microbial metabolic potential

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“…In recent research, Rpf has been shown to boost biotechnological processes such as the degradation of phenol and other contaminants in wastewater ( Jin et al, 2017 ; Li et al, 2018 ; Su et al, 2018a , 2019 ; Yu et al, 2020 ), polychlorinated biphenyls ( Su et al, 2013 , 2015 , 2021 ; Ye et al, 2020 ) and cellulose in soil ( Su et al, 2018b ). Several previously uncultured bacteria with PCB-degrading capabilities were isolated with the aid of Rpf-containing supernatants, specifically high GC-content gram-positive organisms related to the genera Rhodococcus and Arthrobacter , and gram-negative bacteria belonging to the family Alcaligenaceae ( Su et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Potential of Micrococcus luteus Culture Supernatant With Resuscitation-Promoting Factor for Enhancing the Culturability of Soil Bacteria

et al. 2021

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A bacterial species is best characterized after its isolation in a pure culture. This is an arduous endeavor for many soil microorganisms, but it can be simplified by several techniques for improving culturability: for example, by using growth-promoting factors. We investigated the potential of a Micrococcus luteus culture supernatant containing resuscitation-promoting factor (SRpf) to increase the number and diversity of cultured bacterial taxa from a nutrient-rich compost soil. Phosphate-buffered saline and inactivated SRpf were included as controls. After agitation with SRpf at 28°C for 1 day, the soil suspension was diluted and plated on two different solid, oligotrophic media: tenfold diluted Reasoner’s 2A agar (R2A) and soil extract-based agar (SA). Colonies were collected from the plates to assess the differences in diversity between different treatments and cultivation media. The diversity on both R2A and SA was higher in the SRpf-amended extracts than the controls, but the differences on R2A were higher. Importantly, 51 potentially novel bacterial species were isolated on R2A and SA after SRpf treatment. Diversity in the soil extracts was also determined by high-throughput 16S rRNA amplicon sequencing, which showed an increase in the abundance of specific taxa before their successful cultivation. Conclusively, SRpf can effectively enhance the growth of soil bacterial species, including those hitherto uncultured.

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Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Cited by 36 publications

References 43 publications

Strategies for culturing active/dormant marine microbes

Strategies for culturing active/dormant marine microbes

Challenges of unculturable bacteria: environmental perspectives

Exploring the Potential of Micrococcus luteus Culture Supernatant With Resuscitation-Promoting Factor for Enhancing the Culturability of Soil Bacteria

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