Resuscitative effects of polynitroxylated αα-cross-linked hemoglobin following severe hemorrhage in the rat

Buehler, Paul W.; Mehendale, Sangeeta R.; Wang, Huashan; Xie, Jing‐Tian; Ma, Li; Trimble, Charles E.; Hsia, Carleton J. C.; Gulati, Anil

doi:10.1016/s0891-5849(00)00383-x

Cited by 28 publications

(23 citation statements)

References 35 publications

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“…However, if arterial obstruction and lack of oxygen is prolonged, reperfusion with an oxygen carrier alone might also result in ischemia reperfusion injuries. Thus we found that in a rat stroke model, after >60 minutes of ischemia, reperfusion with polyHb resulted in significant increase in the breakdown of the blood-brain barrier and an increase in brain oedema 31 . However, polyHb-SOD-CAT can supply oxygen without causing these adverse changes 31.…”

Section: Crosslinking Of: Hb: Hemoglobinmentioning

confidence: 73%

“…Thus we found that in a rat stroke model, after >60 minutes of ischemia, reperfusion with polyHb resulted in significant increase in the breakdown of the blood-brain barrier and an increase in brain oedema 31 . However, polyHb-SOD-CAT can supply oxygen without causing these adverse changes 31. (Figures 1 and 2) Another way to prevent ischemia-reperfusion injury is the use of polynitroxylated cross-linked Hb…”

Section: Crosslinking Of: Hb: Hemoglobinmentioning

confidence: 73%

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Blood substitutes based on nanobiotechnology

Chang

2006

Trends in Biotechnology

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Stimulated by concerns of potential infective agents in donated blood, since the 1900s, there have been considerable commercial attempts to develop blood substitutes. After many years of development a few of the many leads have shown promise. This review article describes promising nanobiotechnological approaches that are now in Phase III clinical trials. This is followed by a discussion of how important basic knowledge gained is now being used to develop new generations of blood substitutes based on nanobiotechnology. INTRODUCTION:

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Section: Crosslinking Of: Hb: Hemoglobinmentioning

confidence: 73%

Section: Crosslinking Of: Hb: Hemoglobinmentioning

confidence: 73%

Blood substitutes based on nanobiotechnology

Chang

2006

Trends in Biotechnology

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“…Poly SFHb-SOD-CAT has shown the dual function of an oxygen carrier and the ability to remove oxygen radicals, so it can carry oxygen to ischemic brain tissue without causing significant injury in an ischemia-reperfusion rat brain model . This basic research has been extended to a product which is an oxygen carrier with synthetic antioxidant properties (Buehler et al 2004). However, real RBCs are not only O 2 carriers, but also are CO 2 carriers.…”

Section: Discussionmentioning

confidence: 99%

“…This did not induce immunological reaction (Zhu et al 2010). Hsia' s group has extended this to result in a product that is an oxygen carrier with synthetic antioxidant properties (Buehler et al 2004). …”

Section: Introductionmentioning

confidence: 99%

Novel Nanodimension artificial red blood cells that act as O₂and CO₂carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase

Gao

Bian

Chang

2013

Artificial Cells, Nanomedicine, and Biotechnology

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Poly(ethylene glycol)-Poly(lactic acid) block-copolymer (PEG-PLA) was prepared and characterized using Fourier transform infrared spectrophotometer (FTIR). Glutaraldehyde was used to crosslink stroma-free hemoglobin (SFHb), superoxide dismutase (SOD), catalase (CAT), and carbonic anhydrase (CA) into a soluble complex of PolySFHb-SOD-CAT-CA. PEG-PLA was then used to nanoencapsulated PolySFHb-SOD-CAT-CA by oil in water emulsification. This resulted in the formation of PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules that have enhanced antioxidant activity and that can transport both O 2 and CO 2 . These are homogeneous particles with an average diameter of 100 nm with good dispersion and core shell structure, high entrapment efficiency (EE%), and nanocapsule percent recovery. A lethal hemorrhagic shock model in rats was used to evaluate the therapeutic effect of the PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules. Infusion of this preparation resulted in the lowering of the elevated tissue PCO 2 and also recovery of the mean arterial pressure (MAP).

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“…Th is did not induce immunological reaction (Zhu et al 2010). Hsia ' s group has extended this to result in a product that is an oxygen carrier with synthetic antioxidant properties (Buehler et al 2004).…”

Section: Introductionmentioning

confidence: 99%

The Immunological Properties of Stroma-free Polyhemolysate Containing Catalase and Superoxide Dismutase Activities Prepared by Polymerized Bovine Stroma-free Hemolysate

Zhu

Chen

et al. 2010

Artificial Cells, Blood Substitutes, and Biotechnology

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Crosslinking of ultrapure hemoglobin, crystalline catalase, and superoxide dismutase resulted in a soluble nanodimensional complex of polyhemoglobin-catalase-superoxide dismutase. A less expensive and more convenient way is to crosslink bovine stromafree hemolysate (stroma-free hemolysate) that already contains hemoglobin, catalase, and superoxide dismutase into polyhemoglobin with catalase and superoxide dismutase activities (stroma-free polyhemolysate) [21]. The objective of the present study is to evaluate the immunological properties of this stroma-free polyhemolysate. Each of three groups of rats received weekly subcutaneous injections of one of the stroma-free polyhemolysate, stroma-free hemolysate, and saline for four weeks. One week after the four cycles of weekly immunization, serum and plasma were collected for C3a complement activation tests and Ouchterlony antibody-antigen precipitation tests, respectively. Results show that stroma-free polyhemolysate retained signifi cant antioxidant enzyme activity. The C3a complement activation test and Ouchterlony test show that four weekly subcutaneous injections of bovine stroma-free polyhemolysate did not result in any immunological reaction in rats when tested this way

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Resuscitative effects of polynitroxylated αα-cross-linked hemoglobin following severe hemorrhage in the rat

Cited by 28 publications

References 35 publications

Blood substitutes based on nanobiotechnology

Blood substitutes based on nanobiotechnology

Novel Nanodimension artificial red blood cells that act as O₂and CO₂carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase

The Immunological Properties of Stroma-free Polyhemolysate Containing Catalase and Superoxide Dismutase Activities Prepared by Polymerized Bovine Stroma-free Hemolysate

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Resuscitative effects of polynitroxylated αα-cross-linked hemoglobin following severe hemorrhage in the rat

Cited by 28 publications

References 35 publications

Blood substitutes based on nanobiotechnology

Blood substitutes based on nanobiotechnology

Novel Nanodimension artificial red blood cells that act as O2and CO2carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase

The Immunological Properties of Stroma-free Polyhemolysate Containing Catalase and Superoxide Dismutase Activities Prepared by Polymerized Bovine Stroma-free Hemolysate

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Product

Resources

About

Novel Nanodimension artificial red blood cells that act as O₂and CO₂carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase