Resveratrol addition to Chinese hamster ovary cell culture media: The effect on cell growth, monoclonal antibody synthesis, and its chemical modification

Abstract

The effect of the addition of resveratrol to cell culture media during the production of monoclonal antibodies was investigated. Treatments of Chinese hamster ovary (CHO) cells expressing immunoglobulin G (IgG) with 25 and 50 µM resveratrol showed that resveratrol was capable of slowing cell growth whilst almost doubling cell specific productivity to 4.7 ± 0.6 pg IgG/cell⋅day, resulting in up to a 1.37-fold increase of the final IgG titre. A resveratrol concentration of 50 µM slowed the progression through the cell cycle temporarily by trapping cells in the S-phase. Cation exchange chromatography showed no significant difference in the composition of acidic or basic IgG species and size exclusion chromatography indicated no change in fragmentation or aggregation of the recombinant IgG in the treatment groups. Resveratrol could be used as a chemical additive to CHO media where it would enhance IgG productivity and provide a degree of protection against hydroxyl and superoxide free radicals, expanding the range of options for process improvement available to monoclonal antibody manufacturers.

Insights into the Impact of Rosmarinic Acid on CHO Cell Culture Improvement through Transcriptomics Analysis

“…The use of antioxidants in CHO cell cultures to improve monoclonal antibody production has been a topic of great interest [8,[33][34][35][36][37][38][39]. Nevertheless, the precise mechanisms by which RA impacts CHO cell growth, metabolism, and productivity need further elucidation.…”

Application of metabolic modeling for targeted optimization of high seeding density processes

“…Further reduction of ROS species via media optimization of antioxidant compounds such as vitamins, thiols, or α‐ketoacids as well as genetic engineering approaches could further improve culture longevity in HSD processes (Chevallier et al, 2020; Henry et al, 2020). At the same time strategies to induce cell cycle arrest and thereby increase specific productivities via temperature shifts or media components such as sodium butyrate, pentanoic acid, or resveratrol (Kumar et al, 2007; McHugh et al, 2020; Toronjo‐Urquiza et al, 2019) could enhance the productivity of HSD cultures.…”

[Retracted] Analysis of Sino‐Russian Media Cooperation and the Construction of International Online Public Opinion Discourse under the Dual Influence of Ecological and Online Environments

“…China-EU relations have generally maintained stable development, and there are common contradictions based on common interests. At the same time, China actively carries out multilateral diplomacy, participates in the settlement of international hot issues, takes the initiative to assist countries with relatively backward development, and assumes the international responsibility of a big country [ 10 , 11 ]. In recent years, the Chinese concept of the “the Belt and Road” and the “community of shared future for mankind” has won the attention of relevant countries since it was put forward.…”