Resveratrol administration increases phagocytosis, decreases oxidative burst, and promotes pro-inflammatory cytokine production in healthy dogs

Mathew, Leanne M.; Woode, Rowena; Axiak‐Bechtel, Sandra M.; Amorim, Juliana; DeClue, Amy E.

doi:10.1016/j.vetimm.2018.07.013

Cited by 9 publications

(6 citation statements)

References 24 publications

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“…It occurs in high concentrations in grape skins, peanuts and wines and possesses anti-inflammatory, anti-cancerogen and antioxidant properties [8,9,10,11]. Studies involving resveratrol treatment in animals with inflammatory diseases have demonstrated downregulation of inflammation-induced biomarkers, including pro-inflammatory mediators ( IL-1β , IL-6 , TNF-α , MCP-1 , IFN-γ , and NF-κB ) [12,13,14,15] and upregulation of inflammation-reduced biomarkers, including anti-oxidant protein (SOD) and total WBC counts [16,17]. Calabrese et al [18] classified resveratrol as a hormetic modulator in diseases, which included inflammation and infections.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs

Liang

Zhou

et al. 2019

Animals

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Simple SummaryChina’s livestock industry has been transforming from traditional extensive systems to highly intensive systems. Highly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol alleviates inflammation in lambs. Results showed that resveratrol attenuated the LPS-evoked inflammatory responses in lambs by suppressing expression levels of inflammatory cytokines and blocking NF-κB and MAPK signaling pathways. Based on these studies, resveratrol has the potential to be a promising therapeutic reagent for multiple inflammatory illnesses caused by immune stress. AbstractHighly intensive livestock production often causes immune stress to animals, which makes them more susceptible to infections. The aim of this study was to examine whether resveratrol (Res) alleviates inflammation in lambs. In Experiment 1, 16 male lambs were injected with lipopolysaccharides (LPS) at an initial dose of 0.25, 1.25, and 2.5 μg/kg body weight (BW) for 9 days. Average daily gain and blood parameters were measured and clinical symptoms were recorded. In Experiment 2, 20 male lambs were injected intravenously with LPS (0 mg/kg) + Res (0 mg), LPS (2.5 μg /kg) + Res (0 mg, 82.5 mg, 165 mg, 330 mg), 4 h after LPS injection. Jugular blood was collected from each lamb to determine white blood cell (WBC) counts and the expression of inflammatory genes. In Experiment 1, all LPS-treated lambs showed clinical signs of sickness including rhinorrhea, lethargy, and shivering, and systemic inflammatory responses of increased inflammatory genes levels and cortisol concentration. The lambs had increased respiratory and heart rates and rectal temperature and decreased average daily gain and feed intake. In Experiment 2, resveratrol significantly reduced WBCs and the expression levels of several genes associated with inflammation response (TLR4, NF-κB, c-jun) and inhibited the signaling cascades of NF-κB and MAPKs by down-regulating the expression levels of inflammatory cytokines (IL-1β, IL-4, IL-6, TNF-α, IFN-γ) induced by LPS. Resveratrol attenuated the LPS-evoked inflammatory responses in lambs by suppressing expression levels of inflammatory cytokines, and blocking NF-κB and MAPK signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs

Liang

Zhou

et al. 2019

Animals

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“…In that study, resveratrol performed similarly to or more effectively than phenylbutazone and/or flunixin meglumine in reducing lymphocyte derived interferon gamma and TNF-α [33]. Canine in vivo work indicated that short-term oral resveratrol supplementation resulted in increased phagocytosis by PMNs, blunted oxidative burst capacity, increased PAMP-stimulated TNF and IL-6 production and no effect on IL-10 [34]. It is argued that the preserved effects of PMN phagocytosis and oxidative burst functions represent a desirable outcome in our study as those functions are a critical first line host defense to eliminate pathogens and combat infections [35].…”

Section: Discussionmentioning

confidence: 85%

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Martin¹,

Johnson²,

Amorim³

et al. 2020

IJMS

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Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer’s recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.

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“…Oxidative burst and phagocytic activities were determined using commercial kits (Phagoburst and Phagotest kits, respectively; Glycotope Biotechnology). Both kits have been used successfully in previous canine studies 23,30,33 and were used according to the manufacturer’s instructions. Briefly, oxidative burst activity was determined by incubating 2 samples of 100 µL of precooled (0°C) heparinized whole blood, one with opsonized Escherichia coli bacteria and one with phorbol-12-myristate 13-acetate (PMA), at 37°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis

et al. 2021

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Canine parvoviral enteritis (CPE) is a severe disease characterized by systemic inflammation and immunosuppression. The function of circulating phagocytes (neutrophils and monocytes) in affected dogs has not been fully investigated. We characterized the functional capacity of canine phagocytes in CPE by determining their oxidative burst and phagocytic activities using flow cytometry. Blood was collected from 28 dogs with CPE and 11 healthy, age-matched, control dogs. Oxidative burst activity was assessed by stimulating phagocytes with opsonized Escherichia coli or phorbol 12-myristate 13-acetate (PMA) and measuring the percentage of phagocytes producing reactive oxygen species and the magnitude of this production. Phagocytosis was measured by incubating phagocytes with opsonized E. coli and measuring the percentage of phagocytes containing E. coli and the number of bacteria per cell. Complete blood counts and serum C-reactive protein (CRP) concentrations were also determined. Serum CRP concentration was negatively and positively correlated with segmented and band neutrophil concentrations, respectively. Overall, no differences in phagocyte function were found between dogs with CPE and healthy control dogs. However, infected dogs with neutropenia or circulating band neutrophils had decreased PMA-stimulated oxidative burst activity compared to healthy controls. Additionally, CPE dogs with neutropenia or circulating band neutrophils had decreased PMA- and E. coli–stimulated oxidative burst activity and decreased phagocytosis of E. coli compared to CPE dogs without neutropenia or band neutrophils. We conclude that phagocytes have decreased oxidative burst and phagocytic activity in neutropenic CPE dogs and in CPE dogs with circulating band neutrophils.

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Resveratrol administration increases phagocytosis, decreases oxidative burst, and promotes pro-inflammatory cytokine production in healthy dogs

Cited by 9 publications

References 24 publications

Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs

Protective Effect of Resveratrol Improves Systemic Inflammation Responses in LPS-Injected Lambs

Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study

Oxidative burst and phagocytic activity of phagocytes in canine parvoviral enteritis

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