Resveratrol Affects Protein Kinase C Activity and Promotes Apoptosis in Human Colon Carcinoma Cells

Fang, Jieyu; Li, Zhihua; Li, Qiang; Huang, Wen‐Sheng; Kang, Liang; Wang, Jianping

doi:10.7314/apjcp.2012.13.12.6017

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…Resveratrol (15 μM) inhibited TPA-induced PKC expression in human mammary and oral epithelial cells [128], inhibited TPA-induced expression of PKCδ and reduced the metastatic potential of human cervical cancer at 50 μM [129], and affects protein kinase C activity and promotes apoptosis in human colon carcinoma cells at a concentration as high as 200 μM [130]. Resveratrol suppresses the activity of kinases such as protein kinase C (PKC), mitogen-activated protein kinases (MAPK) and IκB kinase (IKK) and transcription factors such as hypoxia-inducible factor-1α (HIF-1α), signal transducer and activator of transcription 3 (STAT3), nuclear factor κB (NF-κB) and activator protein-1 (AP-1) leading to various conditions in response to oncogenic stimuli (Figure 9) [127].…”

Section: Pkc Modulation By Polyphenolsmentioning

confidence: 99%

Polyphenol compounds and PKC signaling

Das

Ramani

Suraju

2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background Naturally occurring polyphenols found in food sources provide huge health benefits. Several polyphenolic compounds are implicated in the prevention of disease states, such as cancer. One of the mechanisms by which polyphenols exert their biological actions is by interfering in the protein kinase C (PKC) signaling pathways. PKC belongs to a superfamily of serine-threonine kinase and are primarily involved in phosphorylation of target proteins controlling activation and inhibition of many cellular processes directly or indirectly. Scope of review Despite the availability of substantial literature data on polyphenols' regulation of PKC, no comprehensive review article is currently available on this subject. This article reviews PKC-polyphenol interactions and its relevance to various disease states. In particular, salient features of polyphenols, PKC, interactions of naturally occurring polyphenols with PKC, and future perspective of research on this subject are discussed. Major conclusions Some polyphenols exert their antioxidant properties by regulating the transcription of the antioxidant enzyme genes through PKC signaling. Regulation of PKC by polyphenols is isoform dependent. The activation or inhibition of PKC by polyphenols has been found to be dependent on the presence of membrane, Ca2+ ion, cofactors, cell and tissue types etc. Two polyphenols, curcumin and resveratrol are in clinical trials for the treatment of colon cancer. General significance The fact that 74% of the cancer drugs are derived from natural sources, naturally occurring polyphenols or its simple analogs with improved bioavailability may have the potential to be cancer drugs in the future.

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Section: Pkc Modulation By Polyphenolsmentioning

confidence: 99%

Polyphenol compounds and PKC signaling

Das

Ramani

Suraju

2016

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…One major function of PTEN is to negatively regulate PI3K/Akt signaling. The PI3K/Akt signaling pathway is one of the essential pathways for cell survival and proliferation, which is over-activated in many human cancers, such as breast, colon and prostate cancers (10,14). Thus, we investigated whether PI3K/Akt signaling is involved in the anti-proliferation effect of Res in HCT116 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Resveratrol (3,5,4'-trihydroxystilbene, Res), as a natural polyphenolic compound mostly from beans and grapes, was discovered in red wine by Siemann and Creasy in 1992 (11), and can be used as inhibitor for platelet aggregation, cardiacprotection and as anticancer agent (10,12). It has been reported also that Res can inhibit diet induced obesity (13) inhibiting proliferation and promoting apoptosis in various tumor cells, such as colon cancer, breast cancer and prostate cancer cells in vitro and in vivo (14)(15)(16)(17)(18)(19)(20). For colon cancer cells, the anti-proliferation and apoptotic inducing effects of Res have already been validated, but the mechanism underlying these activities remains unclear.…”

Section: Introductionmentioning

confidence: 99%

The PTEN/PI3K/Akt and Wnt/β-catenin signaling pathways are involved in the inhibitory effect of resveratrol on human colon cancer cell proliferation

Liu

Huang

et al. 2014

International Journal of Oncology

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Colon cancer is one of the most common malignancies and the treatments for colon cancer have been developed substantially in the last decades, but there is still a great clinical need to explore new treatment regimens due to the undesirable prognosis. In this investigation, we demonstrated the anti-proliferative and apoptosis-inducing activities of resveratrol (Res) in human colon cancer cells, and the possible mechanisms underlying these effects. We used crystal violet staining, flow cytometry and western blotting to validate the anti-proliferative and apoptosis-inducing effects of Res on HCT116 cells. A xenograft tumor model was used to confirm the anti-proliferative effects of Res. We employed polymerase chain reaction, western blotting, recombinant adenovirus and luciferase reporter assay to explore the possible mechanism(s) of action. We found that Res inhibits significantly the proliferation and promotes apoptosis in HCT116 cells, as well as inhibits the xenograft tumor growth of colon cancer. Res upregulates the expression of phosphatase and tensin homolog (PTEN) and decreases the phosphorylation of Akt1/2. The exogenous expression of PTEN inhibits the PI3K/Akt signal and promotes the anti-proliferative effects of Res in HCT116 cells, while knockdown of PTEN increases PI3K/Akt signal but reduces the anti-proliferative function of Res. The protein and mRNA expression of β-catenin are all decreased by Res concentration-dependently. Thus, our findings strongly suggest that the anti-proliferative effects of Res in human colon cancer cells may be mediated by regulating separately the PTEN/PI3K/Akt and Wnt/β-catenin signaling.

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“…In tradition Chinese medicine, different herbs or different component of one herb are assumed to mix together, in such way, different component work coordinately, some shortcomes of one active component are made up by another one (s). This mutual promotion and restraint between the elements theory are commonly used in Chinese medicine (Fang et al, 2012). Thus, we explore the anti-tumor effect of mixture alkaloids by n-butanol extraction, the mechanism of underlying this are studied as well.…”

Section: Discussionmentioning

confidence: 99%

Alkaloids from Beach Spider Lily (Hymenocallis littoralis) Induce Apoptosis of HepG-2 Cells by the Fas-signaling Pathway

Yu-bin

Chen²,

Zhu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose (0.8μg/ml) significantly inhibiting proliferation . The nontumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.

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Resveratrol Affects Protein Kinase C Activity and Promotes Apoptosis in Human Colon Carcinoma Cells

Cited by 17 publications

References 33 publications

Polyphenol compounds and PKC signaling

Polyphenol compounds and PKC signaling

The PTEN/PI3K/Akt and Wnt/β-catenin signaling pathways are involved in the inhibitory effect of resveratrol on human colon cancer cell proliferation

Alkaloids from Beach Spider Lily (Hymenocallis littoralis) Induce Apoptosis of HepG-2 Cells by the Fas-signaling Pathway

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