Resveratrol ameliorates long-term structural, functional and metabolic perturbations in a rat model of donor nephrectomy: Implication of SIRT1

Basta, Marianne; Dief, Abeer E.; Ghareeb, Doaa A.; Saleh, Samar R.; Elshorbagy, Amany K.; Eter, Eman El

doi:10.1016/j.jff.2019.04.043

Cited by 4 publications

(26 citation statements)

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“…The present study showed that UNX induced significant body weight reduction and compensatory renal hypertrophy as well as renal (Basta et al, 2019;Rodríguez-Gómez et al, 2012;Zhao et al, 2008), yet the cognitive, behavioural and brain changes occurring post-nephrectomy are not fully elucidated.…”

Section: Discussionmentioning

confidence: 65%

“…Few studies have addressed the importance of administration of reno-protective agents to UNX rats. A previous study of resveratrol administration to UNX rats showed similar suggesting protective effects of resveratrol on the remaining kidney (Basta et al, 2019) promoting an antioxidant effect and decreasing lipid peroxidation (Özcelik et al, 2012). Zn also downregulated kidney expression of profibrotic growth factors, pro-inflammatory and pro-apoptotic proteins that increase in renal diseases (Piao et al, 2019).…”

Section: Discussionmentioning

confidence: 77%

“…Homogenization of kidney and hippocampus was done on ice using RIPA buffer. The homogenates were centrifuged at 10,000 g for 10 min at 4 • C. The supernatant was collected and stored at −80 • C (Basta et al, 2019).…”

Section: Termination and Tissue Collectionmentioning

confidence: 99%

“…Since rats respond at a faster rate to uninephrectomy (UNX), UNX rats have been used to mimic kidney donation in humans (Basta et al., 2019 ). Animal studies have demonstrated that UNX imposes a risk of developing chronic kidney disease.…”

Section: Introductionmentioning

confidence: 99%

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Possible protective effect of zinc administration on renal and cognitive changes occurring in uninephrectomized adult male Wistar rats

Basta

Yassin

Sayed

2022

Experimental Physiology

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New Findings What is the central question of this study?Are renal changes occurring post‐nephrectomy accompanied by cognitive changes, and does early administration of zinc supplements such as ZnSO4 to uninephrectomized rats ameliorate the renal and cognitive changes if present? What is the main finding and its importance?Uninephrectomy‐induced renal changes were accompanied by species‐atypical behaviour in rats in both Morris water maze and T maze tests, together with hypozincaemia and hippocampal inflammatory and oxidative changes. Early zinc administration to uninephrectomized rats ameliorated the renal, behavioural, hippocampal and serum zinc changes. Abstract Cognitive impairment is increasingly recognized as an important consequence of kidney disease in humans. Kidney donation is a safe procedure but is known to increase the long‐term risk of cardiovascular and kidney disease. Whether kidney donation impairs cognitive function is not known. In the present study, we examined whether the renal changes occurring post‐nephrectomy were accompanied by cognitive changes as well, and whether early administration of zinc supplements such as ZnSO4 to uninephrectomized (UNX) rats could ameliorate the renal and cognitive changes if present. The present study included 30 adult male Wistar rats that were randomly assigned to three groups (n = 10 per group): sham‐operated rats, UNX and UNX treated with ZnSO4 for 20 weeks. Before termination, rats were subjected to 24‐h urine collection and behavioural testing with the Morris water maze and T maze tests. UNX induced significant proteinuria, renal functional, fibrotic and oxidative changes, as well as increased renal desmin expression. UNX rats also showed significant behavioural changes indicating spatial learning and memory affection, together with decreased hippocampal brain derived neurotrophic factor (BDNF) and antioxidant capacity, and increased glial fibrillary acidic protein (GFAP), nitric oxide and malondialdehyde. In addition, UNX induced significant hyperglycaemia and dyslipidaemia, as well as significant reduction in serum zinc, copper and selenium. Early administration of ZnSO4 starting 1 week post‐nephrectomy significantly ameliorated renal and behavioural changes, as well as hippocampal oxidative, BDNF and GFAP changes. Additionally, Zn recovered serum changes of triglycerides, cholesterol, zinc and copper. Therefore, early administration of zinc to humans undergoing nephrectomy may be of benefit and should be considered in human trials.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 77%

Section: Termination and Tissue Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Possible protective effect of zinc administration on renal and cognitive changes occurring in uninephrectomized adult male Wistar rats

Basta

Yassin

Sayed

2022

Experimental Physiology

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“…The following formula, (total cholesterol—HDL-C), was used to calculate non HDL cholesterol (Non HDLc). Atherogenic index was calculated according to the following formula: (Total cholesterol—HDL-C)/HDL-C (Basta et al 2019 ).…”

Section: Methodsmentioning

confidence: 99%

Trivalent chromium supplementation ameliorates adjuvant induced rheumatoid arthritis through up-regulation of FOXP3 and decrease in synovial Cathepsin G expression

et al. 2022

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Background Rheumatoid arthritis (RA) is a known debilitating autoimmune disease. Immune-suppressants that are used for disease treatment have serious side effects, therefore, trivalent chromium (Cr (III)); which has shown evidence of its influences on some inflammatory pathways and cytokines; was used in this study for the first time to be assessed for its therapeutic effect in RA rat model and was compared to prednisolone in a trial to find a treatment with lesser side effects. Methods Adult male albino rats were randomly divided into four groups: normal, untreated RA, prednisolone treated RA (1.25 mg/kg/day) and Cr (III) treated RA groups (80 μg/kg/day), induction of RA was done by subcutaneous complete Freund adjuvant injection. Study duration was 4 weeks throughout which arthritis scoring and weight measurement were pursued. Histopathological examination and immunohistochemical FOXP3 assessment were done for joint biopsies. Serum inflammatory markers (interleukin 17, interleukin 10, CRP) and synovial erosive arthritis marker (Cathepsin G) were measured. HDL and non-HDL cholesterol were estimated as well. Results Cr (III) treatment showed marked clinical and histopathological improvement, also astonishing anti-inflammatory effects (increase in FOXP3 expression and interleukin 10, with decrease in interleukin 17, CRP and synovial Cathepsin G) to the extent that Cr (III) effects on inflammation abolishment were comparable to that of prednisolone and even better at some aspects. Moreover, Cr (III) was protective from side effects, i.e., weight gain and dyslipidemia that were seen with prednisolone treatment. Conclusions Cr (III) is promising in treating RA and it lacks some side effects of accustomed immune-modulatory agents including prednisolone. Further experimental studies and clinical trials should be held to see the efficacy of Cr (III) in different doses and to assess its long term side effects when used for rheumatoid arthritis and other autoimmune diseases treatment.

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Resveratrol ameliorates the behavioural and molecular changes in rats exposed to uninephrectomy: role of hippocampal SIRT1, BDNF and AChE

2022

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Subtle memory and cognitive changes may occur in uninephrectomized (Unix) patients long before the development of chronic kidney disease, such changes may be unnoticed. The dietary polyphenol, Resveratrol, displayed various neuroprotective effects, its role in chronic kidney disease is an area of intense studies. This work was designed to investigate the behavioural and molecular changes that may occur following 7 months of Unix in rats, and to determine whether Resveratrol intake can improve such pathology. Male Wistar rats were divided into three groups: sham operated, Unix and Unix group treated with Resveratrol (20 mg/kg/day). Rats were subjected to series of behavioural testing, different biochemical parameters along with RT-PCR and immunohistochemistry of the hippocampal tissue to track the development of functional or structural brain changes. Anxiety behaviour and reduced spatial memory performance were observed in rats 7 months post-nephrectomy; these deficits were remarkably reversed with Resveratrol. Among the species typical behaviour, burrowing was assessed; it showed significant impairment post-nephrectomy. Resveratrol intake was almost able to increase the burrowing behaviour. Decreased SIRT1 in immune-stained sections, oxidative stress, inflammatory changes, and increased AChE activity in hippocampal homogenates were found in Unix rats, and Resveratrol once more was capable to reverse such pathological changes. This work has investigated the occurrence of behavioural and structural brain changes 7 months following Unix and underlined the importance of Resveratrol to counterbalance the behavioural impairment, biochemical and brain pathological changes after uninephrectomy. These findings may raise the possible protective effects of Resveratrol intake in decreased kidney function.

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Resveratrol ameliorates long-term structural, functional and metabolic perturbations in a rat model of donor nephrectomy: Implication of SIRT1

Cited by 4 publications

References 54 publications

Possible protective effect of zinc administration on renal and cognitive changes occurring in uninephrectomized adult male Wistar rats

Possible protective effect of zinc administration on renal and cognitive changes occurring in uninephrectomized adult male Wistar rats

Trivalent chromium supplementation ameliorates adjuvant induced rheumatoid arthritis through up-regulation of FOXP3 and decrease in synovial Cathepsin G expression

Resveratrol ameliorates the behavioural and molecular changes in rats exposed to uninephrectomy: role of hippocampal SIRT1, BDNF and AChE

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