Resveratrol ameliorates the physiological, biochemical, cytogenetic, and anatomical toxicities induced by copper(II) chloride exposure in Allium cepa L.

Macar, Tuğçe Kalefetoğlu; Macar, Oksal; Yalçın, Emine; Çavuşoğlu, Kültiğin

doi:10.1007/s11356-019-06920-2

Cited by 24 publications

(12 citation statements)

References 53 publications

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“…Our previous finding that Cu toxicity increased the ratio of cell wall biomass to the dry weight of citrus roots is in line with present results [ 20 ]. Similar findings have also been reported in excessive-Cu treated roots of Pinus pinaster Ait (Arduini et al, 1995), Arabidopsis thaliana [ 48 ] and Allium cepa L. [ 49 ]. The comparison between Figure 6 C,G implies that C. grandis maintained a much more integral vacuole structure.…”

Section: Discussionsupporting

confidence: 85%

Physiological and Ultrastructural Responses to Excessive-Copper-Induced Toxicity in Two Differentially Copper Tolerant Citrus Species

Lin

et al. 2023

Plants

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Over-applied copper (Cu)-based agrochemicals are toxic to citrus trees. However, less information is available discussing the ultrastructural alterations in Cu-stressed citrus species. In the present study, seedlings of Citrus sinensis and Citrus grandis that differed in Cu-tolerance were sandy-cultured with nutrient solution containing 0.5 µM Cu (as control) or 300 µM Cu (as Cu toxicity) for 18 weeks. At the end of the treatments, the physiological parameters and ultrastructural features of the citrus leaves and roots were analyzed. The results indicate that Cu toxicity significantly decreased the ratio of shoot biomass to dry weight, the Cu translocation factor and the total chlorophyll of two citrus species. The anatomical and ultrastructural alterations verified that excessive Cu resulted in starch granules accumulated in the leaves and roots of the two citrus species. Under Cu toxicity, increased root flocculent precipitate and thickened root cell wall might reduce the Cu translocation from citrus roots to the shoots. Compared with C. sinensis, C. grandis maintained a relatively integral root cellular structure under Cu toxicity, which provided a structural basis for a higher Cu tolerance than C. sinensis. The present results increase our understanding of the physiological and ultrastructural responses to Cu toxicity in citrus species.

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Section: Discussionsupporting

confidence: 85%

Physiological and Ultrastructural Responses to Excessive-Copper-Induced Toxicity in Two Differentially Copper Tolerant Citrus Species

Lin

et al. 2023

Plants

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“…1,4-dioxane induced peroxidation in membrane component lipids, disruption of DNA integrity by MN and CAs formations are the possible explanation for nuclear shape changes in Group IV. Although there is no study on the effect of 1,4-dioxane on anatomical damage, many studies have shown that many toxic agents cause changes and necrosis in epidermis and cortex cells [44,45]. With previous analyzes of this study it was found that 1,4-dioxane induced genotoxic effects and abnormalities in antioxidant system were found to be reduced by tresv.…”

Section: Discussionsupporting

confidence: 55%

“…treatment with t-resv caused a significant decrease in anatomical changes especially in the frequency of flattened cell nucleus. Similar, Macar et al [45] reported that 400 mg/L and 800 mg/L resveratrol application alleviate the anatomic damages in meristematic cells induced by CuCl 2 .…”

Section: Discussionmentioning

confidence: 55%

Possible protective effects of trans-resveratrol against 1,4-dioxane induced toxicity in meristematic cells of plant bioassays

Kurt¹,

Yalçın²,

Çavuşoğlu³

2020

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Background In this study, the protective effects of trans-resveratrol against 1,4-dioxane induced toxicity in meristematic cells were investigated. For this purpose, Allium test which is a reliable test was used and the alterations in all experimental groups were examined by using physiological, cytogenetic, biochemical and anatomical parameters.Results As a result of the analysis, it has been determined that 1,4-dioxane causes serious abnormalities in Allium cepa meristematic cells. It was determined that in 1,4-dioxane treated group germination percentage regressed 1.6-times, root length reduced 12.7-times and weight gain decreased 7.7-times compared to control group. It has been observed that trans-resveratrol administration with 1,4-dioxane causes improvement in physiological parameters and reduces the damage rate from 0.4 to 0.16. Mitotic index, micronucleus and chromosomal abnormality frequency were investigated as cytogenetic parameters. It was determined that mitotic index decreased, chromosomal abnormalities and micronucleus frequency increased in 1,4-dioxane treated group. Trans-resveratrol treatment was found to cause a dose dependent improvement in genotoxic effects induced by 1,4-dioxane. Changes in the antioxidant system in all experimental groups were determined by measuring malondialdehyde, glutathione, superoxide dismutase and catalase enzyme levels. It was determined that 1,4-dioxane administration caused an increase in malondialdehyde level, decreased glutathion rate and induced antioxidant enzyme activity. Trans-resveratrol application was found to cause improvement in these alterations induced by 1,4-dioxane. It was observed that the 200 mg/mL trans-resveratrol+1,4-dioxane treatment caused a 1.9-fold decrease in malondialdehyde level which is indicator of lipid peroxidation compared to only 1,4-dioxane treated group. The abnormalities caused by 1,4-dioxane application in the meristematic cells are also found in the anatomical structure. In 1,4-dioxane treatment group, anatomical changes such as cell deformation and cortex wall thickening were observed. The frequency of these changes decreased with trans-resveratrol administration.Conclusions As a result, it was determined that 1,4-dioxane caused a versatile toxicity in Allium cepa meristematic cells, while trans-resveratrol was found to have a dose-dependent protective feature against 1,4-dioxane-induced toxicity.

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“…47 ETR represents the rate of photosynthetic electron transfer, which decreases linearly with increasing exposure to Cd due to the interruption of the electron transport chain. 48 49 To investigate the effect of Cd on the ROS levels in A. philoxeroides, the levels of MDA and H 2 O 2 in leaves, stems and roots were monitored and shown in Figure 3A,B. The results indicate that the MDA and H 2 O 2 levels both increased proportionally with the Cd concentration.…”

Section: Enrichment Capacity Of CD In a Philoxeroides The Results In ...mentioning

confidence: 99%

Physiological Changes and Antioxidative Mechanisms of Alternanthera philoxeroides in Phytoremediation of Cadmium

et al. 2023

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This study evaluated the physiological characteristics (e.g., growth parameters, chlorophyll content, metabolites and antioxidative enzymes activity) of Alternanthera philoxeroides (A. philoxeroides), as a hyperaccumulator plant, during the phytoremediation of cadmium (Cd) from water. After cultivating A. philoxeroides in a Cd-containing medium for 30 days, the growth rate was inhibited by up to 33.5% as the exposed Cd concentration increased to 0.80 mmol•L −1 . Cd exposure interfered with the photosynthesis of A. philoxeroides and caused oxidative stress as indicated by the rise of malondialdehyde (MDA) and H 2 O 2 , which increased by 8 times and 3 times compared to the control group. Moreover, high exposure concentrations of Cd also reduced the activities of multiple antioxidants (e.g., GSH and AsA), indicating the inhibition of Cd on the plant's ability to mitigate oxidative damage. Finally, the fluorescent patterns of the rhizosphere dissolved organic matter (rDOM) revealed three major components (humic, fulvic substances and protein-like substances) well correlated with the changes in antioxidant activities. Partial least-squares discriminant analysis (PLS-DA) visualized the difference in the activity of the antioxidative enzymes between different groups. The study unravelled deep insights into the potential mechanisms of tolerance and resistance of A. philoxeroides for phytoremediation of Cd pollution.

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Resveratrol ameliorates the physiological, biochemical, cytogenetic, and anatomical toxicities induced by copper(II) chloride exposure in Allium cepa L.

Cited by 24 publications

References 53 publications

Physiological and Ultrastructural Responses to Excessive-Copper-Induced Toxicity in Two Differentially Copper Tolerant Citrus Species

Physiological and Ultrastructural Responses to Excessive-Copper-Induced Toxicity in Two Differentially Copper Tolerant Citrus Species

Possible protective effects of trans-resveratrol against 1,4-dioxane induced toxicity in meristematic cells of plant bioassays

Physiological Changes and Antioxidative Mechanisms of Alternanthera philoxeroides in Phytoremediation of Cadmium

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