Resveratrol and Curcumin Improve Intestinal Mucosal Integrity and Decrease m⁶A RNA Methylation in the Intestine of Weaning Piglets

Abstract

N6-Methyladenosine (m6A) is the most prevalent modification on eukaryotic messenger RNA (mRNA). Resveratrol and curcumin, which can exert many health-protective effects, may have a relationship with m6A RNA methylation. We hypothesized that the combination of resveratrol and curcumin could affect growth performance, intestinal mucosal integrity, m6A RNA methylation, and gene expression in weaning piglets. One hundred and eighty piglets weaned at 28 ± 2 days were fed a control diet or supplementary diets (300 mg/kg of antibiotics; 300 mg/kg of each resveratrol and curcumin; 100 mg/kg of each resveratrol and curcumin; 300 mg/kg of resveratrol; 300 mg/kg of curcumin) for 28 days. The results showed that the combination of resveratrol and curcumin improved growth performance and enhanced intestinal mucosal integrity and functions in weaning piglets. Resveratrol and curcumin also increased intestinal antioxidative capacity and mRNA expression of tight junction proteins. Furthermore, resveratrol and curcumin decreased the content of m6A and decreased the enrichment of m6A on the transcripts of tight junction proteins and on heme oxygenase-1 in the intestine. Our findings indicated that the combination of resveratrol and curcumin increased growth performance, enhanced intestine function, and protected piglet health, which may be associated with changes in m6A methylation and gene expression, suggesting that curcumin and resveratrol may be a potential natural alternative to antibiotics.

Resveratrol Attenuates Aflatoxin B1-Induced ROS Formation and Increase of m6A RNA Methylation

“…In addition, mice in the RES group exhibited a significant reduction of METTL3 protein expression and a prominent increase of FTO protein expression. Consistent with our previous study, resveratrol was able to reduce the abundance of m 6 A modification in piglets [47]. Therefore, these data suggest that the protective function of resveratrol against AFB 1 -induced liver damage is related to the reduction of m 6 A modification.…”

Comparison of the protective effects of resveratrol and pterostilbene against intestinal damage and redox imbalance in weanling piglets

“…Between 21 and 28 days of age, they were fed a basal diet supplemented with: 300 mg/kg of resveratrol (W-RSV), 300 mg/kg of pterostilbene (W-PT), or no supplementation (W-CON). The dosages of resveratrol and pterostilbene added to the diet of weaned piglets were selected based on an independent pre-study, as well as the results reported by our colleagues and other groups [29,30]. A greater degree of feed efficiency was observed for weaned piglets between the ages of 21 and 42 days when pterostilbene was provided at 250 or 500 mg/kg of diet (unpublished).…”

“…A greater degree of feed efficiency was observed for weaned piglets between the ages of 21 and 42 days when pterostilbene was provided at 250 or 500 mg/kg of diet (unpublished). Resveratrol has been proven to improve the jejunal antioxidant ability of weaned piglets at 42 days of age and prevent the muscular lipid peroxidation of finishing pigs at slaughter [29,30]. Therefore, the same dose, 300 mg/kg, was selected for both resveratrol and pterostilbene in this study.…”

Molecular Iodine Has Extrathyroidal Effects as an Antioxidant, Differentiator, and Immunomodulator

“…Another possibility currently explored in our laboratory is that I 2 as an oxidized agent can exert epigenetic modifications associated with the activation of important demethylase enzymes like DMT3 [ 101 ], unpublished results). In fact, other natural antioxidants, such as resveratrol or curcumin, exert part of their effects by modifying the methylation/demethylation equilibrium genes for the differentiation of Th1 (T-bet and STAT4) or Th2 (GATA3) [ 102 ].…”