Resveratrol and its antifungal activity against Candida species

Weber, Kai; Schulz, Bettina; Ruhnke, Markus

doi:10.1111/j.1439-0507.2009.01763.x

Cited by 47 publications

(29 citation statements)

References 11 publications

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“…The effect of resveratrol (3,5,4-trihydroxystilbene), a close analogue of PTE, on C. albicans has been investigated previously (57)(58)(59). However, its antifungal effect was much weaker than PTE.…”

Section: Met14mentioning

confidence: 99%

“…However, its antifungal effect was much weaker than PTE. More specifically, Okamoto et al revealed that the MIC 50 of resveratrol against C. albicans SC5314 was about 200 g/ml, and resveratrol (Ն40 g/ml) could inhibit the yeast-to-hypha morphological transition of C. albicans (57), while Collado et al (58) and Weber et al (59) reported that resveratrol had no antifungal activity against C. albicans. In this study, we found that PTE has a much stronger antifungal effect against C. albicans than resveratrol, with a MIC 80 of 32 g/ml and a minimal concentration in- hibiting morphological transition of 4 g/ml.…”

Section: Met14mentioning

confidence: 99%

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In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Zhao

Mylonakis

et al. 2014

Antimicrob Agents Chemother

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d Pterostilbene (PTE) is a stilbene-derived phytoalexin that originates from several natural plant sources. In this study, we evaluated the activity of PTE against Candida albicans biofilms and explored the underlying mechanisms. In 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assays, biofilm biomass measurement, confocal laser scanning microscopy, and scanning electron microscopy, we found that <16 g/ml PTE had a significant effect against C. albicans biofilms in vitro, while it had no fungicidal effect on planktonic C. albicans cells, which suggested a unique antibiofilm effect of PTE. Then we found that PTE could inhibit biofilm formation and destroy the maintenance of mature biofilms. At 4 g/ ml, PTE decreased cellular surface hydrophobicity (CSH) and suppressed hyphal formation. Gene expression microarrays and real-time reverse transcription-PCR showed that exposure of C. albicans to 16 g/ml PTE altered the expression of genes that function in morphological transition, ergosterol biosynthesis, oxidoreductase activity, and cell surface and protein unfolding processes (heat shock proteins). Filamentation-related genes, especially those regulated by the Ras/cyclic AMP (cAMP) pathway, including ECE1, ALS3, HWP1, HGC1, and RAS1 itself, were downregulated upon PTE treatment, indicating that the antibiofilm effect of PTE was related to the Ras/cAMP pathway. Then, we found that the addition of exogenous cAMP reverted the PTE-induced filamentous growth defect. Finally, with a rat central venous catheter infection model, we confirmed the in vivo activity of PTE against C. albicans biofilms. Collectively, PTE had strong activities against C. albicans biofilms both in vitro and in vivo, and these activities were associated with the Ras/cAMP pathway.

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Section: Met14mentioning

confidence: 99%

Section: Met14mentioning

confidence: 99%

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Zhao

Mylonakis

et al. 2014

Antimicrob Agents Chemother

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“…Among dietary polyphenols, resveratrol (3,4’,5-trihydroxy-trans-stilbene), found in the skin of grapes, blueberries, raspberries and mulberries [9], is known to be effective against bacterial and fungal infections [10, 11], cardiovascular diseases [12], metabolic diseases [13], and cancer [14–16]. Resveratrol has also been reported to show anti-inflammatory effects [17], inhibit lipid peroxidation [18], and activate free radical scavenging [19], further supporting the evidence in the favor of the antioxidant activity of resveratrol.…”

Section: Introductionmentioning

confidence: 99%

Trans-scirpusin A showed antitumor effects via autophagy activation and apoptosis induction of colorectal cancer cells

et al. 2017

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Trans-Scirpusin A (TSA) is a resveratrol oligomer found in Borassus flabellifer L. We found that TSA inhibited the growth of colorectal cancer Her2/CT26 cells in vivo in mice. Although some cytotoxic T lymphocytes (CTLs) were induced against the tumor-associated antigen Her2, TSA treatment did not significantly increase the level of Her2-specific CTL response compared to that with vehicle treatment. However, there was a significant increase in the level of TNF-α mRNA in tumor tissue and Her2-specific Ab (antibody) production. More importantly, we found that TSA overcomes the tumor-associated immunosuppressive microenvironment by reducing the number of CD25+FoxP3+ regulatory T cells and myeloid-derived suppressor cells (MDSCs). We detected the induction of autophagy in TSA-treated Her2/CT26 cells, based on the increased level of the mammalian autophagy protein LC3 puncta, and increased conversion of LC3-I to LC3-II. Further, TSA induced 5' AMP-activated protein kinase (p-AMPK) (T172) and inhibited mammalian target of rapamycin complex 1 (mTORC1) activity as estimated by phosphorylated ribosomal protein S6 kinase beta-1 (p-p70S6K) levels, thereby suggesting that TSA-mediated AMPK activation and inhibition of mTORC1 pathway might be associated with autophagy induction. TSA also induced apoptosis of Her2/CT26 cells, as inferred by the increased sub-G1 mitotic phases in these cells, Annexin V/PI-double positive results, and TUNEL-positive cells. Finally, we found that the combined treatment of mice with docetaxel and TSA successfully inhibited tumor growth to a greater extent than docetaxel alone. Therefore, we propose the use of TSA for supplementary anticancer therapy to support anti-neoplastic drugs, such as docetaxel, by inducing apoptosis in cancer cells and resulting in the induction of neighborhood anti-cancer immunity.

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“…In terms of the activity of resveratrol against C. albicans, there is still some controversy. Weber et al showed resveratrol did not show any significant effects on several Candida species [32]. However, the latest report suggested that resveratrol derivatives could affect on Candida species [13].…”

Section: Introductionmentioning

confidence: 97%

Novel Antifungal Mechanism of Resveratrol: Apoptosis Inducer in Candida albicans

Lee

2014

Curr Microbiol

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Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a well-known natural polyphenolic compound that has garnered considerable interest because of its bioavailability and pharmacokinetics in humans. Although the antimicrobial activity of resveratrol has recently been focused, however, the antifungal activity and its mechanism are still largely unknown. Here, we report for the first time the potential of resveratrol as an apoptosis inducer in the human pathogenic fungus Candida albicans. The results showed that resveratrol exerted its effects from the early to the late stages of apoptosis and involved the activity of reactive oxygen species, particularly hydroxyl radicals ((∙)OH). DiOC6(3) and JC-1 staining indicated that loss of mitochondrial membrane potential (ΔΨ m) is a key event in resveratrol-induced apoptosis. Finally, we investigated metacaspase activation resulting from mitochondrial dysfunction. The result showed that resveratrol strongly activated metacaspase and promoted cytochrome c release. In summary, resveratrol induces fungal apoptosis through a caspase-dependent mitochondrial pathway and is a potential agent for treating human fungal diseases.

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Resveratrol and its antifungal activity against Candida species

Cited by 47 publications

References 11 publications

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

In Vitro and In Vivo Activities of Pterostilbene against Candida albicans Biofilms

Trans-scirpusin A showed antitumor effects via autophagy activation and apoptosis induction of colorectal cancer cells

Novel Antifungal Mechanism of Resveratrol: Apoptosis Inducer in Candida albicans

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