Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

Schwager, Joseph; Bompard, Albine; Raederstorff, Daniel; Hug, Hubert; Bendik, Igor

doi:10.3390/biomedicines10071524

Cited by 9 publications

(8 citation statements)

References 59 publications

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“…The protein encoded by CCR7 is a member of the G protein-coupled receptor family (25)(26)(27) and is up-regulated in response to inflammation, pathogens, and tissue damage (28). CCR7 chemokine axis is composed of chemokine ligands CCL19 and CCL21, and chemokine receptor CCR7 (29,30).…”

Section: Discussionmentioning

confidence: 99%

Shared gene characteristics and molecular mechanisms of macrophages M1 polarization in calcified aortic valve disease

Qin

Chen²,

Li³

et al. 2023

Front. Cardiovasc. Med.

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BackgroundCAVD is a common cardiovascular disease, but currently there is no drug treatment. Therefore, it is urgent to find new and effective drug therapeutic targets. Recent evidence has shown that the infiltration of M1 macrophages increased in the calcified aortic valve tissues, but the mechanism has not been fully elucidated. The purpose of this study was to explore the shared gene characteristics and molecular mechanisms of macrophages M1 polarization in CAVD, in order to provide a theoretical basis for new drugs of CAVD.MethodsThe mRNA datasets of CAVD and M1 polarization were downloaded from Gene Expression Omnibus (GEO) database. R language, String, and Cytoscape were used to analyze the functions and pathways of DEGs and feature genes. Immunohistochemical staining and Western Blot were performed to verify the selected hub genes.ResultsCCR7 and GZMB were two genes appeared together in hub genes of M1-polarized and CAVD datasets that might be involved in the process of CAVD and macrophages M1 polarization. CCR7 and CD86 were significantly increased, while CD163 was significantly decreased in the calcified aortic valve tissues. The infiltration of M1 macrophages was increased, on the contrary, the infiltration of M2 macrophages was decreased in the calcified aortic valve tissues.ConclusionThis study reveals the shared gene characteristics and molecular mechanisms of CAVD and macrophages M1 polarization. The hub genes and pathways we found may provide new ideas for the mechanisms underlying the occurrence of M1 polarization during CAVD process.

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Section: Discussionmentioning

confidence: 99%

Shared gene characteristics and molecular mechanisms of macrophages M1 polarization in calcified aortic valve disease

Qin

Chen²,

Li³

et al. 2023

Front. Cardiovasc. Med.

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“…Resveratrol has been studied for its anti‐inflammatory effects on blood cells, macrophage/monocyte and endothelial cell lines after induction of the inflammatory response (Schwager et al, 2017). Recent findings indicate that resveratrol strengthens the macrophage‐mediated effects on the innate and adaptive immune response (Schwager et al, 2022). For these reasons, the results obtained from our experiments in vitro are in agreement with the literature, showing that the nutraceutical formulation decreases the release of inflammatory mediators.…”

Section: Discussionmentioning

confidence: 99%

Nutraceutical formulation for immune system modulation: Active constituents, in vitro antibacterial and immunomodulatory activity, and metabolomics analysis

Faggian,

Bernabè,

Pauletto

et al. 2023

Phytotherapy Research

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There is a large demand for nutraceuticals in the market and studies related to their action are needed. In this paper, the antimicrobial activity and the immunomodulatory effect of a nutraceutical formulation containing 14.39% of ascorbic acid, 7.17% of coenzyme Q10, 1.33% of Echinacea polyphenols, 0.99% of pine flavan‐3‐ols, 0.69% of resveratrol and 0.023% of Echinacea alkylamides were studied using in vitro assays and cell‐based metabolomics. Chromatographic analysis allowed us to study the nutraceutical composition. The antibacterial activity was evaluated on S. aureus, K. pneumoniae, P. aeruginosa, E. coli, H. influenzae, S. pyogenes, S. pneumoniae and M. catarrhalis. The immunomodulatory activity was assessed on human macrophages and dendritic cells. The production of IL‐1β, IL‐12p70, IL‐10 and IL‐8 was evaluated on culture medium by ELISA and the activation/maturation of dendritic cells with cytofluorimetric analysis. Treated and untreated macrophages and dendritic cell lysates were analysed by liquid chromatography coupled with high‐resolution mass spectrometry, and results were compared using multivariate data analysis to identify biological markers related to the treatment with the food supplement. The food supplement decreased K. pneumoniae, P. aeruginosa, E. coli, Methicillin‐resistant Staphylococcus aureus (MRSA) and M. catharralis growth, reduced the inflammatory response in macrophages exposed to lipopolysaccharide (LPS) and modulated the activation and maturation of the dendritic cells. Oxidized phospholipids were identified as the main biological markers of treated cell lysates, compared with controls.

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“…This could be a consequence of subtle cellular activation. In activated THP-1 cells, CXCL10 levels can increase up to 1000-fold [ 30 ], while activated HL-60 cells increase CXCL10 negligibly [ 31 ], pointing to different response pathways in these cell lines from monocytic compared to granulocytic precursors, respectively. Both IL-8 and CXLC10 act as chemoattractants.…”

Section: Discussionmentioning

confidence: 99%

Heat Shock Protein 27 Affects Myeloid Cell Activation and Interaction with Prostate Cancer Cells

et al. 2022

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Heat shock proteins are cytoprotective molecules induced by environmental stresses. The small heat shock protein 27 (Hsp27) is highly expressed under oxidative stress conditions, mediating anti-oxidative effects and blocking apoptosis. Since medical gas plasma treatment subjects cancer cells to a multitude of reactive oxygen species (ROS), inducing apoptosis and immunomodulation, probable effects of Hsp27 should be investigated. To this end, we quantified the extracellular Hsp27 in two prostate cancer cell lines (LNCaP, PC-3) after gas plasma-induced oxidative stress, showing a significantly enhanced release. To investigate immunomodulatory effects, two myeloid cell lines (THP-1 and HL-60) were also exposed to Hsp27. Only negligible effects on viability, intracellular oxidative milieu, and secretion profiles of the myeloid cells were found when cultured alone. Interestingly, prostate cancer-myeloid cell co-cultures showed altered secretion profiles with a significant decrease in vascular endothelial growth factor (VEGF) release. Furthermore, the myeloid surface marker profiles were changed, indicating an enhanced differentiation in co-culture upon Hsp27 treatment. Finally, we investigated morphological changes, proliferation, and interaction with prostate cancer cells, and found significant alterations in the myeloid cells, supporting the tendency to differentiate. Collectively, our results suggest an ambiguous effect of Hsp27 on myeloid cells in the presence of prostate cancer cells which needs to be further investigated.

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Resveratrol and ω-3 PUFAs Promote Human Macrophage Differentiation and Function

Cited by 9 publications

References 59 publications

Shared gene characteristics and molecular mechanisms of macrophages M1 polarization in calcified aortic valve disease

Shared gene characteristics and molecular mechanisms of macrophages M1 polarization in calcified aortic valve disease

Nutraceutical formulation for immune system modulation: Active constituents, in vitro antibacterial and immunomodulatory activity, and metabolomics analysis

Heat Shock Protein 27 Affects Myeloid Cell Activation and Interaction with Prostate Cancer Cells

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