Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

Abstract

Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.

Study on the mechanism of peanut resistance to Fusarium oxysporum infection induced by Bacillus thuringiensis TG5

“…After induction by B. thuringiensis TG5 and carbendazim, peanut plants secrete glutamyl-s-cysteine sulfoxide and 4,8-dimethyldeca-4,8-dieonic acid, respectively, as defense means. Some bioactive compounds in peanuts ( Kuei et al, 2021 ; Park et al, 2021 ; Duke, 2022 ; Li et al, 2022 ), including resveratrol ( Luo et al, 2021 ; Zhu et al, 2022 ), β-sitosterol, proanthocyanidin, linoleic acid and arachidonic glycosides have inhibitory effects on plant pathogenic bacteria and fungi. To further explore the metabolic pathway and mechanism of microbial pesticides inducing peanut disease resistance, and the difference in disease resistance between microbial pesticides and traditional chemical pesticide carbendazim, provide data and theoretical support for the development and promotion of microbial pesticides, which is of great practical significance for sustainable agricultural development.…”

Effects of Carbohydrates on Rosmarinic Acid Production and In Vitro Antimicrobial Activities in Hairy Root Cultures of Agastache rugosa

“…Indeed, hairy roots have been obtained from many plant sources for the production of most groups of secondary metabolites, including phenolics, alkaloids, glucosinolates, terpenoids, and others. Many previous studies have confirmed that hairy roots can be a good source of plant-derived bioactive compounds, with flavonoid (anthocyanin) accumulation in hairy roots of Fagopyrum tataricum [ 17 ], flavone accumulation in hairy roots of Scultellaria baicalensis [ 18 ], ginsenoside production in hairy roots of Platycodon grandiflorum [ 19 ], glucosinolate production in hairy roots of Nasturtium officinale [ 20 ], resveratrol in hairy roots of Arachis hypogaea [ 21 ], tropane alkaloids in hairy roots of Hyoscyamus reticulatus [ 22 ], and tanshinone in hairy roots of Salvia miltiorrhiza [ 23 ].…”