Resveratrol inhibits human nasopharyngeal carcinoma cell growth via blocking pAkt/p70S6K signaling pathways

Abstract

Abstract. 4' , has been shown to exert potent anticancer effects on various types of cancer through its anti-proliferative, anti-angiogenic, antioxidant and pro-apoptotic functions. There is still a lack of experimental evidence regarding whether resveratrol has potential anticancer activity in human nasopharyngeal carcinoma (NPC) cells. The purpose of this study was to explore the anticancer activity of resveratrol in human NPC cells both in vitro and in vivo. Our results indicated that treatment with resveratrol led to a time-and dose-dependent decrease in cell proliferation in NPC cells. A dose-dependent increase in apoptosis in response to resveratrol treatment was also observed in NPC cells. Flow cytometric analysis showed that treatment of NPC cells with resveratrol led to cell cycle arrest at the S and G2/M phases. Mechanistically, resveratrol treatment downregulated the expression of Bcl-2 and hypoxia-inducible factor-1α (HIF-1α) proteins and upregulated the expression of caspase-3 protein. In addition, resveratrol treatment also significantly decreased the phosphorylation levels of Akt1, p70S6K and p-4E-BP-1 and the protein expression of several cyclins involved in cell cycle regulation. In vivo studies further showed that resveratrol was able to significantly inhibit the growth of NPC tumor xenografts in nude mice. Collectively, our findings suggest that resveratrol exerts potent anti-proliferative and pro-apoptotic effects on human NPC cells possibly through interfering with the pAkt1/p70S6K signaling pathways, thus it may potentially be developed as an effective agent for chemoprevention and chemotherapy of human NPC.

Biological significance and therapeutic implication of resveratrol-inhibited Wnt, Notch and STAT3 signaling in cervical cancer cells

“…And STAT3 signaling is constitutively activated in cervical cancer and may play potential role in progression of HPV16-mediated cervical carcinogenesis [36]. A body of evidence demonstrates that the multifaceted biological activities of resveratrol are reflected by its inhibitory effects on the activations of cancer-associated signaling pathways [37-39]. For these reasons, the statuses of Wnt, Notch and STAT3 signaling in HeLa and SiHa cells without and with resveratrol treatment were investigated in currently.…”

Effect of Processing, Post-Harvest Irradiation, and Production System on the Cytotoxicity and Mutagenicity of Vitis labrusca L. Juices in HTC Cells

“…are included in this class and possess many medicinal properties. They can be used to treat infections [2], cardiovascular diseases [3] and hyperglycemia [4], and they can serve as chemopreventive [5], chemotherapeutic [6], antioxidant [7] and antimutagenic [8] agents.…”

The pharmacology of resveratrol in animals and humans

“…RSV treatment reduced tumor growth of human nasopharyngeal carcinoma cells (CNE-2Z cell line) [118], head and neck cancer-derived tumor-initiating cells (HNC-TICs) [119], and head and neck squamous cell carcinoma (HNSCC) cells (FaDu cell line) [120], in nude mouse xenograft models. With the anti-tumor effect of against head and neck cancer, RSV suppressed tumor stemness by reducing the expression of stemness markers (Oct4 and Nestin) and mesenchymal-like protein (Vimentin), and induced epithelial protein (Ecadherin) [119], increased cleaved casepase-3 (an apoptotic marker) and γ-histone 2AX (a DNA damage marker) [120].…”