Resveratrol inhibits the hydrogen dioxide-induced apoptosis via Sirt 1 activation in osteoblast cells

Abstract

Sirt 1 plays a critical role in stress responses. We determined the deregulation of Sirt 1 activity, p53 acetylation, Bcl-2 expression, and mitochondria-dependent apoptosis in mouse osteoblast MC3T3-E1 cells which were exposed to H2O2. And then we investigated the protective role of Sirt 1 activator, Resveratrol (RSV), against the H2O2-induced apoptosis. Results demonstrated that Sirt 1 and Bcl-2 were inhibited, whereas p53 acetylation, Bax, and caspase 9 were promoted by H2O2, as was aggravated by the Sirt 1 inhibitor, EX-527. Instead, RSV inhibited the H2O2-induced both p53 acetylation and the caspase 9 activation, whereas ameliorated the H2O2-induced Bcl-2 inhibition and apoptosis. In conclusion, Sirt 1 was downregulated during the H2O2-induced apoptosis in MC3T3-E1 cells. And the chemical activation of Sirt 1 inhibited the H2O2-induced apoptosis via the downregulation of p53 acetylation. Our results suggest that Sirt 1 upregulation appears to be an important strategy to inhibit the oxidative stress-induced apoptosis.

Effect of the three Enterococcus faecalis strains on apoptosis in MC3T3 cells

“…Further investigations are needed to explore the relevance of the CRISPR‐cas system and the effect of the E. faecalis strains on apoptosis in osteoblasts. In addition, the expression of Bcl‐2/Bax can be decreased to promote osteoblast apoptosis by glucocorticoids (Lucinda et al., ), sodium fluoride (Yang et al., ), bilirubin (Ruiz‐Gaspa et al., ) and H 2 O 2 (He et al., ), in contrast to the elevated level of Bcl‐2/Bax in saved osteoblast protected by puerarin (Liu et al., ), vaspin (Zhu et al., ) and ghrelin (Liang et al., ). Lipopolysaccharides (LPS) of Escherichia coli can also decrease the Bcl‐2 expression and increase the Bax expression in the MC3T3 cells (Guo et al., ).…”

Exogenous Hydrogen Sulfide Postconditioning Protects Isolated Rat Hearts From Ischemia/Reperfusion Injury Through Sirt1/PGC-1α Signaling Pathway

“…These effects were largely reversed by the addition of Ex-527, a specific inhibitor of Sirt1. The dosage of Ex-527 was selected based on previous reports [20][21][22] and our preliminary experiments.…”

Grape seed proanthocyanidins prevent H 2 O 2 ‐induced mitochondrial dysfunction and apoptosis via SIRT 1 activation in embryonic kidney cells

“…In HEK‐293 cells, H 2 O 2 caused a significant reduction in the expression of SIRT 1 and increased expression of SIRT 3. H 2 O 2 has already been shown to inhibit SIRT 1 (He et al, ; Xu et al, ) and increase SIRT 3 (Dai et al, ) expressions. Our study show, for the first time, that SIRT1 is the target of GSPE against H 2 O 2 ‐induced sirtuin dysregulation.…”