Resveratrol loaded in cationic glucosylated liposomes to treat Staphylococcus epidermidis infections

Pagano, Livia; Gkartziou, Foteini; Aiello, Stefano; Simonis, Beatrice; Ceccacci, Francesca; Sennato, Simona; Ciogli, Alessia; Mourtas, Spyridon; Spiliopoulou, Iris; Antimisiaris, Sophia G.; Bombelli, Cecilia; Mancini, Giovanna

doi:10.1016/j.chemphyslip.2022.105174

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…DOPC was chosen because-on the basis of our experience-a phosphocholine with unsaturated chains allows us to easily obtain monodisperse RSV-loaded liposomes functionalized with amphiphiles [13,30]. As already explained by Aiello et al [13], cholesterol in the lipid mixture enhances the stability of the lipid bilayer through the bilayer-tightening effect, inducing dense packing and increasing the orientation order of lipid chains.…”

Section: Liposome Preparationmentioning

confidence: 99%

“…The formulations were stable for two weeks during storage, without any significant change in size and PDI, except for LGR liposomes, for which a decrease in ζ-potential was observed. This effect may be due to the migration of RSV towards the surface, thus towards a more hydrated environment: in these conditions, RSV can partially undergo ionization, with the negatively charged hydroxyl group oriented towards the lipid water interface, thus making the potential less positive [30].…”

Section: Stability Studiesmentioning

confidence: 99%

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Resveratrol and Resveratrol-Loaded Galactosylated Liposomes: Anti-Adherence and Cell Wall Damage Effects on Staphylococcus aureus and MRSA

Prevete,

Simonis,

Mazzonna

et al. 2023

Biomolecules

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Antibiotic resistance due to bacterial biofilm formation is a major global health concern that makes the search for new therapeutic approaches an urgent need. In this context,, trans-resveratrol (RSV), a polyphenolic natural substance, seems to be a good candidate for preventing and eradicating biofilm-associated infections but its mechanism of action is poorly understood. In addition, RSV suffers from low bioavailability and chemical instability in the biological media that make its encapsulation in delivery systems necessary. In this work, the anti-biofilm activity of free RSV was investigated on Staphylococcus aureus and, to highlight the possible mechanism of action, we studied the anti-adherence activity and also the cell wall damage on a MRSA strain. Free RSV activity was compared to that of RSV loaded in liposomes, specifically neutral liposomes (L = DOPC/Cholesterol) and cationic liposomes (LG = DOPC/Chol/GLT1) characterized by a galactosylated amphiphile (GLT1) that promotes the interaction with bacteria. The results indicate that RSV loaded in LG has anti-adherence and anti-biofilm activity higher than free RSV. On the other side, free RSV has a higher bacterial-growth-inhibiting effect than encapsulated RSV and it can damage cell walls by creating pores; however, this effect can not prevent bacteria from growing again. This RSV ability may underlie its bacteriostatic activity.

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Section: Liposome Preparationmentioning

confidence: 99%

Section: Stability Studiesmentioning

confidence: 99%

Resveratrol and Resveratrol-Loaded Galactosylated Liposomes: Anti-Adherence and Cell Wall Damage Effects on Staphylococcus aureus and MRSA

Prevete,

Simonis,

Mazzonna

et al. 2023

Biomolecules

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“…Liposomal encapsulation of resveratrol has been shown to improve the solubility, stability, and bioavailability of RSV, enhancing its therapeutic effects, such as the anti-cancer properties [ 215 ], antibacterial properties [ 216 ], wound healing properties [ 217 ], reduction in induced nephrotoxicity [ 218 ], among others properties, by protecting it from degradation and facilitating its delivery to targeted cells or tissues.…”

Section: Rsv Modificationsmentioning

confidence: 99%

Enhancing the Bioavailability of Resveratrol: Combine It, Derivatize It, or Encapsulate It?

Salla,

Karaki,

El Kaderi

et al. 2024

Pharmaceutics

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Overcoming the limited bioavailability and extensive metabolism of effective in vitro drugs remains a challenge that limits the translation of promising drugs into clinical trials. Resveratrol, despite its well-reported therapeutic benefits, is not metabolically stable and thus has not been utilized as an effective clinical drug. This is because it needs to be consumed in large amounts to overcome the burdens of bioavailability and conversion into less effective metabolites. Herein, we summarize the more relevant approaches to modify resveratrol, aiming to increase its biological and therapeutic efficacy. We discuss combination therapies, derivatization, and the use of resveratrol nanoparticles. Interestingly, the combination of resveratrol with established chemotherapeutic drugs has shown promising therapeutic effects on colon cancer (with oxaliplatin), liver cancer (with cisplatin, 5-FU), and gastric cancer (with doxorubicin). On the other hand, derivatizing resveratrol, including hydroxylation, amination, amidation, imidation, methoxylation, prenylation, halogenation, glycosylation, and oligomerization, differentially modifies its bioavailability and could be used for preferential therapeutic outcomes. Moreover, the encapsulation of resveratrol allows its trapping within different forms of shells for targeted therapy. Depending on the nanoparticle used, it can enhance its solubility and absorption, increasing its bioavailability and efficacy. These include polymers, metals, solid lipids, and other nanoparticles that have shown promising preclinical results, adding more “hype” to the research on resveratrol. This review provides a platform to compare the different approaches to allow directed research into better treatment options with resveratrol.

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“…Those result from infection with a methicillin-resistant strain containing a slime capsule as a natural barrier. This also prevents other antibiotics from entering the cell, including rifamycin, fluoroquinolones, gentamicin, tetracycline, chloramphenicol, erythromycin, clindamycin, and sulfonamides [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying S. epidermidis

Hlaoperm

Sudjarwo

Ehrenbrandtner

et al. 2023

Sensors

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Staphylococcus epidermidis (S. epidermidis) belongs to methicillin-resistant bacteria strains that cause severe disease in humans. Herein, molecularly imprinted polymer (MIP) nanoparticles resulting from solid-phase synthesis on entire cells were employed as a sensing material to identify the species. MIP nanoparticles revealed spherical shapes with diameters of approximately 70 nm to 200 nm in scanning electron microscopy (SEM), which atomic force microscopy (AFM) confirmed. The interaction between nanoparticles and bacteria was assessed using height image analysis in AFM. Selective binding between MIP nanoparticles and S. epidermidis leads to uneven surfaces on bacteria. The surface roughness of S. epidermidis cells was increased to approximately 6.3 ± 1.2 nm after binding to MIP nanoparticles from around 1 nm in the case of native cells. This binding behavior is selective: when exposing Escherichia coli and Bacillus subtilis to the same MIP nanoparticle solutions, one cannot observe binding. Fluorescence microscopy confirms both sensitivity and selectivity. Hence, the developed MIP nanoparticles are a promising approach to identify (pathogenic) bacteria species.

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Resveratrol loaded in cationic glucosylated liposomes to treat Staphylococcus epidermidis infections

Cited by 8 publications

References 36 publications

Resveratrol and Resveratrol-Loaded Galactosylated Liposomes: Anti-Adherence and Cell Wall Damage Effects on Staphylococcus aureus and MRSA

Resveratrol and Resveratrol-Loaded Galactosylated Liposomes: Anti-Adherence and Cell Wall Damage Effects on Staphylococcus aureus and MRSA

Enhancing the Bioavailability of Resveratrol: Combine It, Derivatize It, or Encapsulate It?

Molecularly Imprinted Nanoparticle Ensembles for Rapidly Identifying S. epidermidis

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