Resveratrol loaded native silk fiber-sericin hydrogel double interpenetrating bioactive wound dressing facilitates full-thickness skin wound healing

Abstract

Wound repair is challenging for traditional wound dressings. New bioactive dressings need to be developed urgently. Herein, we reported a highly bioactive silk protein wound dressing (SPD) with natural silk fiber-sericin hydrogel interpenetrating double network structure, which combines the dual characteristics of natural silk and sericin hydrogel. Silk fiber scaffolds were secreted directly from silkworms bred by regulating their spinning behaviors. Sericin in SPD is obtained by dissolving silkworm cocoons at high temperature and high pressure, while it remains intact activities to self-assemble a hydrogel. To explore the effect of SPD, we first systematically evaluated its physicochemical properties and biological activities in vitro. The SPD exhibits high porosity, prominent mechanical strength, pH-responsive degradability, and excellent anti-oxidation and cell compatibility. Besides, SPD can load and maintain long-term drug release. Based on the satisfactory performance of SPD in vitro, effective in vivo treatment was achieved in a mouse full-thickness wound model, as demonstrated by a significantly accelerated wound healing process, promote the regeneration of hair follicles and sebaceous glands, increased expression of VEGF, and reduced inflammation. Further, resveratrol was loaded into SPD to enhance the effects of anti-oxidation and anti-inflammation for wound healing. Our investigation shows that SPD with excellent physicochemical and biological properties applied in a murine full-thickness skin wound model resulted in remarkable and efficient acceleration of healing process, which may inspire the design of new, effective, and safer medical materials for tissue regeneration.

Hydrogel-based dressings designed to facilitate wound healing

“…Li et al reported the development of a natural silk fibroin-gelatin hydrogel-based dressing loaded with resveratrol, which was subsequently employed for wound healing applications. 152 Yu and colleagues formulated a CMCS-plantamajoside hydrogel comprising plantamajoside, a natural Chinese herbal medicine known for its antioxidation capabilities. This hydrogel effectively diminished oxidative stress indicators and exhibited enhanced wound healing, as evaluated through an in vivo burn wound model.…”

Melatonin/Sericin Wound Healing Patches: Implications for Melanoma Therapy

“…In recent years, wound healing patches containing sericin have been the center of attention (Table 2). Sericin as a component of the matrix appeared next to polymers: poly (vinyl alcohol) [236], sodium carboxy-methyl-cellulose and polyvinyl alcohol [237], placentaderived extracellular matrix [238], silk fiber [239], PVA/chitosan [240], PVA [241], robust alcohol, polyurethane/chitosan [242], carboxymethyl cellulose [243], poly (N-isopropylacrylamide) [244], cellulose/silk nonwoven fabric [245], collagen-fibrin [246], poly(2-hydroxyethyl methacrylate) [247], cellulose [248], carboxymethyl cellulose as hydrogels [249], cellulose/poly(vinyl alcohol) [250], poly lactide-co-glycolic acid [251], PVA/collagen [252], PVA/chitosan [253], polycaprolactone/cellulose acetate/fibroin [254], poly(ethyleneterephhalate)-g-poly (hydroxyethylmethacrylate, PET-g-HEMA) nanofibers [255], sericin/chitosan/polyvinyl alcohol [256], gelatin [257], fibroin [258], poly(Σ-caprolactone)/poly(ethylene oxide) [259]. There are also sericin-based patches with only sericin or sericin treated with HRP/H 2 O 2 as a matrix, but in significantly smaller amounts [260,261].…”

“…There are also sericin-based patches with only sericin or sericin treated with HRP/H 2 O 2 as a matrix, but in significantly smaller amounts [260,261]. The main active substances in those inventions included resveratrol [239], turmeric [243], silver nanoparticles [255], poly(lactic-co-glycolic acid) nanoparticles [257], and sericin as a sole ingredient [260,261] or in combinations with human placenta [238], silver [244], collagen [246] or azithromycin [250]. The applications of sericin-polymer patches in recent years involved wound treatment [238,239,[243][244][245]249,250,252,254,257,259,261], including acute [237] and infected large burn wounds [236], artificial skin [242], wound dressings [240,241,248,253,255,258], tissue engineering [247], regenerative scaffolds [246], and periodontal tissue engineering [251].…”

Transparent injectable sericin-honey hydrogel with antioxidant and antibacterial activities combined with feeding sericin accelerates diabetic wound healing

“…At present, sericin has been employed to fabricate various bioactive polymers [27,[31][32][33], and preliminary studies have been conducted in the repair of injured skin [27], muscle [29], nerve [34,35], and others. The combination of the merits of sericin with the properties of hydrogels endows the biomaterial with excellent histocompatibility and the capability of creating a moderately moist microenvironment for wounds [36]. Moreover, the porous structure of hydrogel facilitates the loading of cells, drugs, or other biologically active molecules.…”