Resveratrol Modulates Interleukin-1β-induced Phosphatidylinositol 3-Kinase and Nuclear Factor κB Signaling Pathways in Human Tenocytes

Busch, Franziska; Mobasheri, Ali; Shayan, Parviz; Lueders, Cora; Stahlmann, Ralf; Shakibaei, Mehdi

doi:10.1074/jbc.m112.377028

Cited by 89 publications

(71 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, MSC derived from SIRT1 knock-out mice showed clearly reduced chondrogenic potential in vitro (67). Indeed, these results are consistent with earlier reports demonstrating that SIRT1 is able to interact with other transcription factors, like Runx2 in osteoblasts or scleraxis in tenocytes (38,62,68) or p53, NF-B, myogenic differentiation, high mobility group I, E2F transcription factor, peroxisome proliferator-activated receptor-␥, and forkhead box O (27,28,30,69).…”

Section: Discussionsupporting

confidence: 81%

Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells

Buhrmann

Busch

Shayan

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Background: Molecular signaling during chondrogenic differentiation of mesenchymal stem cells (MSC) is poorly understood. Results: Knockdown of sirtuin-1 (SIRT1) in MSC induced inhibition of chondrogenesis, Sox9 expression, up-regulation of nuclear factor-B (NF-B) phosphorylation and acetylation, and NF-B-dependent pro-inflammatory enzymes. Conclusion: SIRT1 supports chondrogenic differentiation of MSCs by Sox9 activation and NF-B deacetylation. Significance: These findings may be essential for improving cartilage tissue regeneration.

show abstract

Section: Discussionsupporting

confidence: 81%

Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells

Buhrmann

Busch

Shayan

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…SH5 is a cell permeable, reversible, substratecompetitive phosphatidylinositol analogue that inhibits AKT activation and the activation of select downstream substrates without decreasing phosphorylation of PDK-1 or other kinases downstream of RAS, such as MAPK. SH5 has been widely used as specific inhibitor of AKT in many cell lines (Lan et al 2011, Busch et al 2012, Qiao et al 2013, Yang et al 2013, acting as a potent inducer of apoptosis that selectively kills various types of cancer cells that contain high levels of active AKT. We used a second inhibitor, a AKT1/2 inhibitor, in additional experiments (Gilot et al 2010).…”

Section: Semen Collection and Processingmentioning

confidence: 99%

Phosphorylated AKT preserves stallion sperm viability and motility by inhibiting caspases 3 and 7

Bolaños¹,

Silva²,

Muñoz³

et al. 2014

Reproduction

View full text Add to dashboard Cite

AKT, also referred to as protein kinase B (PKB or RAC), plays a critical role in controlling cell survival and apoptosis. To gain insights into the mechanisms regulating sperm survival after ejaculation, the role of AKT was investigated in stallion spermatozoa using a specific inhibitor and a phosphoflow approach. Stallion spermatozoa were washed and incubated in Biggers-Whitten-Whittingham medium, supplemented with 1% polyvinyl alcohol (PVA) in the presence of 0 (vehicle), 10, 20 or 30 mM SH5, an AKT inhibitor. SH5 treatment reduced the percentage of sperm displaying AKT phosphorylation, with inhibition reaching a maximum after 1 h of incubation. This decrease in phosphorylation was attributable to either dephosphorylation or suppression of the active phosphorylation pathway. Stallion spermatozoa spontaneously dephosphorylated during in vitro incubation, resulting in a lack of a difference in AKT phosphorylation between the SH5-treated sperm and the control after 4 h of incubation. AKT inhibition decreased the proportion of motile spermatozoa (total and progressive) and the sperm velocity. Similarly, AKT inhibition reduced membrane integrity, leading to increased membrane permeability and reduced the mitochondrial membrane potential concomitantly with activation of caspases 3 and 7. However, the percentage of spermatozoa exhibiting oxidative stress, the production of mitochondrial superoxide radicals, DNA oxidation and DNA fragmentation were not affected by AKT inhibition. It is concluded that AKT maintains the membrane integrity of ejaculated stallion spermatozoa, presumably by inhibiting caspases 3 and 7, which prevents the progression of spermatozoa to an incomplete form of apoptosis.Free Spanish abstract A Spanish translation of this abstract is freely available at

show abstract

“…Furthermore, NF-κB was found to be an important component of the SDF-1/CXCR4 axis (32). NF-κB is a typical transcription factor that is activated through phosphorylation (23). A recent study showed that phosphorylation of NF-κB increases during hypoxia (33).…”

Section: Discussionmentioning

confidence: 99%

“…Res also prevents or mitigates the effects of eye diseases, such as retinal detachment and diabetic retinopathy (19)(20)(21)(22). The main target of Res is nuclear factor (NF)-κB, which is a major factor of the SDF-1/CXCR4 axis (23).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Seong

Ryu

Jeong

et al. 2012

Molecular Medicine Reports

View full text Add to dashboard Cite

Abstract. The present study characterizes the effects of resveratrol (Res) on vascular endothelial growth factor (VEGF) secretion in retinal pigment epithelial (RPE) cells. ARPE-19 cells were treated with CoCl 2 , a hypoxia mimetic agent. CoCl 2 treatment increased protein levels of hypoxia inducible factor-1α (HIF-1α) and CXC-chemokine receptor 4 (CXCR4), and secretion of VEGF. To confirm the effects of Res on VEGF secretion, the human umbilical vein endothelial cell tube formation assay was performed with conditioned medium from Res-treated ARPE-19 cells. The well-known antioxidant Res effectively blocked these effects and reduced phosphorylation of nuclear factor (NF)-κB, an upstream activator of CXCR4.

show abstract

Resveratrol Modulates Interleukin-1β-induced Phosphatidylinositol 3-Kinase and Nuclear Factor κB Signaling Pathways in Human Tenocytes

Cited by 89 publications

References 49 publications

Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells

Sirtuin-1 (SIRT1) Is Required for Promoting Chondrogenic Differentiation of Mesenchymal Stem Cells

Phosphorylated AKT preserves stallion sperm viability and motility by inhibiting caspases 3 and 7

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Contact Info

Product

Resources

About