Resveratrol prevents benzo(a)pyrene-induced disruption of mitochondrial homeostasis via the AMPK signaling pathway in primary cultured neurons

Kang, Run-Run; Sun, Qian; Chen, Kai-Ge; Cao, Qing-Tian; Liu, Chang; Liu, Kuan; Ma, Zhuo; Deng, Yu; Liu, Wei; Xu, Bin

doi:10.1016/j.envpol.2020.114207

Cited by 34 publications

(13 citation statements)

References 37 publications

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“…RSV is capable of stimulates autophagy/mitophagy by activating Sirt1, and therefore has shown great potential in the treatment of several diseases. Pre-treatment with RSV reduced the benzo (a) pyrene-induced mitochondrial dysfunction and apoptosis via mitophagy regulation in neurons (Kang et al, 2020). In support of this, our study showed that RSV treatment strongly ameliorated Bhlhe40-induced LC3 II and LAMP2 inhibition in C17.2 cells.…”

Section: Discussionsupporting

confidence: 84%

Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta

Zhao

Liú

et al. 2021

Front. Cell Dev. Biol.

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Neural tube defects (NTDs) are the most severe congenital malformations that result from failure of neural tube closure during early embryonic development, and the underlying molecular mechanisms remain elusive. Mitophagy is the best-known way of mitochondrial quality control. However, the role and regulation of mitophagy in NTDs have not yet been elucidated. In this study, we used an all-trans retinoic acid (ATRA)-induced rat model to investigate mitophagy and its underlying mechanism in spina bifida aperta (SBA). The results of western blot, immunofluorescence and RT-qPCR analyses indicated that mitophagy was impaired and Sirt1 was downregulated in SBA. Administration of resveratrol-a strong specific Sirt1 activator-activated Sirt1, thus attenuating autophagy suppression and ameliorating SBA. RNA-sequencing and bioinformatics analysis results indicated that transcriptional regulation played an important role in NTDs. A luciferase reporter assay was performed to demonstrate that the transcription factor Bhlhe40 directly bound to and negatively regulated Sirt1 expression. Further, we discovered that the Bhlhe40/Sirt1 axis regulated mitophagy in neural stem cells. Collectively, our results for the first time demonstrate that Bhlhe40/Sirt1 axis regulated mitophagy is implicated in ATRA-induced SBA. Our findings provide new insights into pathogenesis of NTDs and a basis for potential therapeutic targets for NTDs.

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Section: Discussionsupporting

confidence: 84%

Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta

Zhao

Liú

et al. 2021

Front. Cell Dev. Biol.

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“…BaP has been shown to induce apoptosis in primary cultured neurons ( 6 ), HL-7702 human normal liver cells ( 21 ), and rat lung epithelial cells ( 30 ). Metabolism of BaP leads to the formation of BPDE, which can bind to DNA to form BPDE-DNA adducts that may lead to cell apoptosis ( 31 ).…”

Section: Resultsmentioning

confidence: 99%

“…The trial was set up including the following three parts. In the first part, cells were treated with BaP (1, 2, 5, 10, 20, 50 and 100 µM) for different times (3,6,12, 24 and 48 h) and 6 types of polyphenols (1, 2, 5, 10 and 20 µM) for 24 h to evaluate the cytotoxicity of BaP and polyphenols. In the second part, cells were Pre-treated or co-treated with eriodictyol (5, 10 and 20 µM), naringenin (20 µM) and BaP (50 µM) for 24 h to assess the protective effects of the polyphenols on BaP-induced cytotoxicity.…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

“…These results are consistent with the study by Liu et al (29) showing that eriodictyol inhibited epidermal growth factorinduced cell S-phase accumulation and increased the percentage of G1-phase cells. BaP has been shown to induce apoptosis in primary cultured neurons (6), HL-7702 human normal liver cells (21), and rat lung epithelial cells (30). Metabolism of BaP leads to the formation of BPDE, which can bind to DNA to form BPDE-DNA adducts that may lead to cell apoptosis (31).…”

Section: Effect Of Eriodictyol and Naringenin On Bap-induced Cell Cyc...mentioning

confidence: 99%

“…Many studies have shown that polyphenols hold promises for reducing DNA damage, oxidative stress, and carcinogenesis induced by BaP in vitro and in vivo ( 4 , 5 ). In in vitro studies, primary cultured neurons ( 6 ), HepG2 cells ( 7 ) and Bhas 42 cells ( 8 ) have been used to investigate how polyphenols may counteract the detrimental effects of BaP exposure. In addition, several animal studies have reported that oral administration of polyphenols such as quercetin ( 9 ), curcumin ( 10 ), and galangin ( 11 ) elicited protection against BaP-induced damage of the lung and other organs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Proteomic Analysis of the Protective Effect of Eriodictyol on Benzo(a)pyrene-Induced Caco-2 Cytotoxicity

et al. 2022

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We evaluated the possible protective effects of six polyphenols on benzo(a)pyrene (BaP)-induced cytotoxicity in Caco-2 cells. We show that treatment with quinic acid, ferulic acid, homovanillic acid, trolox and BaP decreased cell viability, whereas naringenin and eriodictyol affected viability in a bi-phasic manner with low concentrations decreasing viability whereas higher concentrations increase viability. Co-treatment with 20 μM eriodictyol or naringenin reduced BaP-induced cytotoxicity, including cell apoptosis, cell cycle progression, and oxidative stress. Our results show that the protective effect of eriodictyol was superior to that of naringenin. The potential protective mechanisms of eriodictyol on BaP-induced toxicity were investigated by proteomics. We identified 80 differentially expressed proteins (DEPs) with proteins associated with genetic information processing pathway representing the highest proportion and number of proteins responding to eriodictyol treatment, including key proteins such as RPA2, SNRPA, RAD23B, NUP155 and AARS. Our results provide new knowledge on how polyphenols may prevent BaP-induced carcinogenesis.

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Resveratrol impacts on aquatic animals: a review

Kari,

Téllez-Isaías,

Khoo

et al. 2024

Fish Physiol Biochem

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Resveratrol prevents benzo(a)pyrene-induced disruption of mitochondrial homeostasis via the AMPK signaling pathway in primary cultured neurons

Cited by 34 publications

References 37 publications

Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta

Bhlhe40/Sirt1 Axis-Regulated Mitophagy Is Implicated in All-Trans Retinoic Acid-Induced Spina Bifida Aperta

Proteomic Analysis of the Protective Effect of Eriodictyol on Benzo(a)pyrene-Induced Caco-2 Cytotoxicity

Resveratrol impacts on aquatic animals: a review

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