Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm

Longobardi, V.; Zullo, G.; Salzano, Angela; Canditiis, C. De; Cammarano, Andrea; Luise, Luca De; Puzio, Maria Valeria; Neglia, Gianluca; Gasparrini, B.

doi:10.1016/j.theriogenology.2016.09.046

Cited by 73 publications

(48 citation statements)

References 62 publications

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“…Moreover, Falchi et al [ 123 ] did not find any antioxidant effect of resveratrol on the post-thawed buck semen [ 123 ]. These findings, in agreement with previous studies on buck [ 124 ] and other species [ 125 ], might indicate that the positive effect of resveratrol on thawed semen could be dependent on dose, sperm variables such as concentration used for freezing, animal species, storage procedure, and entity of stressing conditions. As discussed until now, the use of resveratrol in sperm preservation has been extensively related to its supplementation to extenders before cryopreservation.…”

Section: Resveratrol Effects On Malessupporting

confidence: 92%

The Role of Resveratrol in Mammalian Reproduction

et al. 2020

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Resveratrol is one of the most investigated natural polyphenolic compounds and is contained in more than 70 types of plants and in red wine. The widespread interest in this polyphenol derives from its antioxidant, anti-inflammatory and anti-aging properties. Several studies have established that resveratrol regulates animal reproduction. However, the mechanisms of action and the potential therapeutic effects are still unclear. This review aims to clarify the role of resveratrol in male and female reproductive functions, with a focus on animals of veterinary interest. In females, resveratrol has been considered as a phytoestrogen due to its capacity to modulate ovarian function and steroidogenesis via sirtuins, SIRT1 in particular. Resveratrol has also been used to enhance aged oocyte quality and as a gametes cryo-protectant with mainly antioxidant and anti-apoptotic effects. In males, resveratrol enhances testes function and spermatogenesis through activation of the AMPK pathway. Furthermore, resveratrol has been supplemented to semen extenders, improving the preservation of sperm quality. In conclusion, resveratrol has potentially beneficial effects for ameliorating ovarian and testes function.

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Section: Resveratrol Effects On Malessupporting

confidence: 92%

The Role of Resveratrol in Mammalian Reproduction

et al. 2020

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“…It has been reported that Res supplementation in the extender before cryopreservation significantly decreases oxidative stress in buffalo sperm 17 , and Res treatment significantly decreased ROS levels in mouse sperm 11 . As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol significantly improves the fertilisation capacity of bovine sex-sorted semen by inhibiting apoptosis and lipid peroxidation

Zhao

Huang

et al. 2018

Sci Rep

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The aim of this study was to test the effects of five different concentrations (0, 10−3, 10−4, 10−5, and 10−6 M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10−3 M and 10−4 M Res groups were significantly lower than those of controls (P < 0.05); (2) the percentage of viable sperm, percentage of sperm with high Δψm, and the ATP content in 10−3 M and 10−4 M Res groups were significantly higher than those of controls (P < 0.05); (3) the percentage of viable sperm with acrosomal integrity, and the blastocyst percentage and quality of the 10−4 M Res group were significantly higher than those of controls (P < 0.05). In conclusion, 10−4 M Res supplementation in washing and fertilisation medium of sex-sorted bull sperm significantly decreased ROS, PS externalisation, and MDA, and protected mitochondrial function and acrosomal integrity, thereby increasing blastocyst percentage and quality following IVF.

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“…Sperm motility on fresh and frozen/thawed semen was examined by phase‐contrast microscopy (Nikon E200) at 40× magnification on a clean and dry glass slide overlaid with a coverslip and maintained on thermo‐regulated stage at 37°C. Any drifting of the specimen was permitted to stop and the percentage of motile spermatozoa was subjectively determined to the nearest 5% by analysing four to five fields of view (Longobardi et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, cryopreservation, although maintaining sperm viability at subzero temperatures, determines deleterious effects on cell structures resulting in reduced fertility (Pegg, ; Woods, Benson, Agca, & Critser, ). It is known that cryopreservation decreases motility, membrane integrity and mitochondrial activity, induces capacitation‐like changes, hence reducing viability and fertilizing ability (Longobardi et al, ; Parks & Graham, ). It follows that improvement of semen cryopreservation would favourably impact on the efficiency of reproductive technologies intervening on both the paternal and maternal lineages for accelerating genetic progress.…”

Section: Introductionmentioning

confidence: 99%

Effect of caspase inhibitor Z‐VAD‐FMK on bovine sperm cryotolerance

Pagano

Longobardi

Canditiis

et al. 2020

Reprod Domestic Animals

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The aim of this study was to evaluate the treatment of bovine semen with the pan‐caspase inhibitor benzyloxycarbonyl‐Val‐Ala‐Asp‐fluoromethyl ketone (Z‐VAD‐FMK), before or after freezing on semen quality. After the initial assessment, sperm from 4 bulls were pooled (Experiment 1) and cryopreserved in BioXcell containing 0, 20 and 100 μM Z‐VAD‐FMK. After thawing semen viability, motility, membrane integrity, as well as DNA fragmentation and ΔΨm were evaluated. In Experiment 2, bovine frozen/thawed sperm were incubated for 1 hr with 0, 20 and 100 µM Z‐VAD‐FMK before assessing the semen quality. The treatment with Z ‐VAD‐FMK before cryopreservation improved post‐thawing sperm motility compared to the control group (p < .05), while no differences were recorded in sperm viability and membrane integrity among groups (on average 86.8 ± 1.5 and 69.1 ± 1.4, respectively). Interestingly, at the highest concentration, DNA fragmentation decreased (p < .05), while the percentage of spermatozoa with high ΔΨm increased (p < .05). The results of Experiment 2 showed that 1‐hr treatment with Z‐VAD‐FMK did not affect sperm motility and viability (on average 63.4 ± 5.8 and 83.7.1 ± 1.2, respectively). However, Z‐VAD‐FMK improved sperm membrane integrity (p < .05) and at the highest concentration tested decreased the proportion of sperm showing DNA fragmentation (p < .05). No differences were recorded in the percentage of spermatozoa with high ΔΨm (on average 57.0 ± 11.4). In conclusion, the treatment with 100 µM of the caspase inhibitor Z‐VAD‐FMK before freezing increased bovine sperm mass motility and ΔΨm, while decreasing sperm DNA fragmentation. Treatment of semen after thawing with 100 µM Z‐VAD‐FMK improved sperm membrane integrity and reduced DNA fragmentation.

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Resveratrol prevents capacitation-like changes and improves in vitro fertilizing capability of buffalo frozen-thawed sperm

Cited by 73 publications

References 62 publications

The Role of Resveratrol in Mammalian Reproduction

The Role of Resveratrol in Mammalian Reproduction

Resveratrol significantly improves the fertilisation capacity of bovine sex-sorted semen by inhibiting apoptosis and lipid peroxidation

Effect of caspase inhibitor Z‐VAD‐FMK on bovine sperm cryotolerance

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