Resveratrol Promotes Gluconeogenesis by Inhibiting SESN2-mTORC2-AKT Pathway in Calf Hepatocytes

Propionate promotes gluconeogenesis by regulating mechanistic target of rapamycin (mTOR) pathway in calf hepatocytes

“…The gluconeogenesis assay of the hepatocytes was performed as previously described ( Chow and Jesse, 1992 ). Briefly, after 48 h of cell isolation ( Wang et al., 2023 ), the RPMI 1640 medium was replaced with glucose-free medium (11966025, Gibco) containing 5 mM pyruvate. Next, the medium was collected after adding NaP and PA, alone or in combination, for 6 h. After centrifugation, the supernatant was collected, and the glucose content was detected with the Glucose Assay Reagent kit (S0201S; Beyotime, Shanghai, China) according to the manufacturer's instructions.…”