Resveratrol protects human keratinocytes HaCaT cells from UVA-induced oxidative stress damage by downregulating Keap1 expression

Liu, Yong; Chan, Fangxiao; Sun, Hongxia; Yan, Jiang; Fan, Dongying; Zhao, Dongzhi; An, Jing; Zhou, Dujin

doi:10.1016/j.ejphar.2010.10.009

Cited by 109 publications

(97 citation statements)

References 35 publications

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“…Liu et al reported that resveratrol protects HaCat from UVA-induced oxidative stress damage by down-regulating Keap1 expression. [29]. However, in our system the action mechanism of resveratrol and polydatin is still not fully understood.…”

Section: Discussionmentioning

confidence: 89%

Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response

et al. 2012

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Abstract-It is well known that human keratinocytes produce the anti-microbial peptide β-defensin 2. Its production is enhanced by pathogenic microorganisms or other environmental stressors. In this study, we evaluated the effect of resveratrol, a polyphenol found in several dietary source as grape seed, and its natural precursor, polydatin on heat-stressed human keratinocytes. By reverse transcription-polymerase chain reaction and enzyme-linked immunoadsorbent assay, we demonstrated that resveratrol used in combination with polydatin was able to modulate interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha gene expression. In addition, our data show that resveratrol and polydatin increased the heat shock protein (Hsp)70B′ gene expression, a Hsp that plays an important role in the cytoprotection and repair of cells and tissues. Worthy of note, polydatin used alone or in combination with resveratrol, increased the release of human β-defensin 2. These results highlighted the ability of polydatin and resveratrol to reinforce cytoprotective response in stress conditions and suggest their use in cosmetic or pharmaceutical preparations.

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Section: Discussionmentioning

confidence: 89%

Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response

et al. 2012

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“…15) Numerous studies have shown that polyphenols, such as epigallocatechin gallate (EGCG), 16) resveratrol (trans-3,5,4′-trihydroxystilbene), 17) and quercetin (3,5,7,3′,4′-pentahydroxyflavon), 18) prevent UVinduced skin ageing.…”

Section: 11)mentioning

confidence: 99%

“…13,14) Antioxidants are regarded as promising agents that reduce such oxidative stress. In recent years, naturally occurring compounds, such as phenolic acids, flavonoids, and high molecular weight polyphenols, have gained considerable attention as beneficial protective agents.15) Numerous studies have shown that polyphenols, such as epigallocatechin gallate (EGCG), 16) resveratrol (trans-3,5,4′-trihydroxystilbene), 17) and quercetin (3,5,7,3′,4′-pentahydroxyflavon), 18) prevent UVinduced skin ageing.Passion fruit (Passiflora edulis SimS.) is a vine species of the passion flower family (Passifloraceae) indigenous to the tropical regions of America and is known as a medicinal herb.…”

mentioning

confidence: 99%

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Maruki‐Uchida

Kurita

Sugiyama

et al. 2013

Biological & Pharmaceutical Bulletin

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The use of naturally occurring botanicals with substantial antioxidant activity to prevent photoageing is receiving increasing attention. We have previously identified piceatannol and scirpusin B, which is a dimer of piceatannol, as strong antioxidants that are present in passion fruit (Passiflora edulis) seeds. In the present study, the effects of passion fruit seed extract, piceatannol, and scirpusin B on human keratinocytes were investigated. The passion fruit seed extract and piceatannol upregulated the glutathione (GSH) levels in keratinocytes in a dose-dependent manner, indicating that piceatannol is an active component of the passion fruit seed extract in keratinocytes. The pretreatment with piceatannol also supressed the UVB-induced generation of reactive oxygen species (ROS) in the keratinocytes. In addition, the transfer of the medium from the UVBirradiated keratinocytes to non-irradiated fibroblasts enhanced matrix-metalloproteinase (MMP)-1 activity, and this MMP-1 induction was reduced when the keratinocytes were pretreated with piceatannol. These results suggest that piceatannol attenuates the UVB-induced activity of MMP-1 along with a reduction of ROS generation in keratinocytes. Thus, piceatannol and passion fruit seed extract containing high amounts of piceatannol are potential anti-photoageing cosmetic ingredients.Key words piceatannol; passion fruit seed; keratinocyte; ultraviolet; reactive oxygen species; matrix-metalloproteinase Solar UV irradiation damages human skin causing it to age prematurely, a process referred to as photoageing, and to develop the characteristics of thickened epidermis and increased melanogenesis.1-3) In contrast, intrinsic (chronological) ageing is characterised by skin atrophy accompanied by a loss of elasticity and reduced metabolic activity. [4][5][6][7][8] A hallmark of photoageing is the disorganisation of collagen, the major structural component of the skin. Collagen is produced mainly by dermal fibroblasts and is degraded by the matrix metalloproteinases (MMPs) that are secreted by various cells, including keratinocytes, fibroblasts, and inflammatory cells.2,8,9) UV irradiation, particularly UVB (290-320 nm) and UVA (315-400 nm), induces the expression of matrix metalloproteinase-1 (MMP-1) in the fibroblasts, which is mainly responsible for the degradation of the dermal collagen in the ageing process of human skin. Therefore, UV-induced MMP-1 is considered to contribute substantially to the connective tissue damage that causes severe collagen deficiency and wrinkling during photoageing.2,8) Although keratinocytes do not produce MMP-1 in response to UVB irradiation, damaged epidermal keratinocytes indirectly play important roles in the release of MMP-1. It has been reported that the culture medium of UVB-irradiated keratinocytes stimulates MMP-1 release from fibroblasts more efficiently than the direct irradiation of the fibroblasts. 10,11)The primary mechanism by which UV irradiation initiates molecular responses in human skin is through the photochromic genera...

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“…Responding to hydrogen peroxide (H 2 O 2 ) which plays a pivotal role among ROS, keratinocytes trigger apoptosis determined by released cytochrome c, cleaved caspases activities, and proapoptotic gene expressions (Zuliani et al., 2005). The oxidative damaged keratinocytes contribute the pathogenesis of skin‐related diseases such as psoriasis, skin aging, and skin cancer (Bae et al., 2014; Kohen, 1999; Liu et al., 2011), thus emerging studies that antioxidants protect against H 2 O 2 ‐induced apoptosis in human keratinocytes HaCaT (Bae et al., 2014; Nguyen, Kim, & Lee, 2013; Seo & Jeong, 2015). …”

Section: Introductionmentioning

confidence: 99%

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

Yoon

Lee

Song

et al. 2018

Food Science & Nutrition

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Black soybeans are functional foods containing a variety of bioactives such as isoflavones, carotenoids, tocopherols, phenolic acid as well as anthocyanins. Here, we examined whether Cheongja#3 black soybean extract has a protective effect on oxidative stress‐induced cell death in human keratinocytes HaCaT. First, we identified fat‐soluble bioactives in three varieties of soybean extracts (Saedanbaek, Daechan, and Cheongja#3). In particular, black soybean Cheongja#3 had high amounts of lutein than other varieties. We demonstrated that Cheongja#3 extract reduced intracellular reactive oxygen species levels in HaCaT cells. Furthermore, Cheongja#3 protected cells from hydrogen peroxide (H2O2)‐induced oxidative stress and triggered cell death determined by cell viabilities and apoptotic caspase activities. Next, we identified the underlying mechanism is due to increased Nrf2 antioxidant system by Cheongja#3, thus increasing the expression of heme oxygenases (HO)‐1. These results indicated that Cheongja#3 soybean extract has protective role against oxidative stress by upregulating the Nrf‐2 antioxidant system in human keratinocyte HaCaT cells.

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Resveratrol protects human keratinocytes HaCaT cells from UVA-induced oxidative stress damage by downregulating Keap1 expression

Cited by 109 publications

References 35 publications

Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response

Polydatin, A Natural Precursor of Resveratrol, Induces β-Defensin Production and Reduces Inflammatory Response

The Protective Effects of Piceatannol from Passion Fruit (<i>Passiflora edulis</i>) Seeds in UVB-Irradiated Keratinocytes

Black soybeans protect human keratinocytes from oxidative stress‐induced cell death

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