Resveratrol regulates the inflammation and oxidative stress of granulosa cells in PCOS via targeting TLR2

Yuan, Ben; Luo, Shuhong; Feng, Liulian; Wang, Junling; Mao, Junbiao; Luo, Bingbing

doi:10.1007/s10863-022-09942-7

Cited by 13 publications

(10 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, LPS specifically mediates TLR4-activation and initiates downstream pathways, thereby releasing a series of inflammatory factors. In addition, LPS may activate TLR2 receptors and induce higher protein levels, causing an inflammatory response and oxidative stress. − Our previous study confirmed the presence of TLR2 and TLR4 as important receptors for PSPP-1. The results of the present study indicate that LPS treatment markedly increases both TLR2 and TLR4 expressions, whereas PSPP-1 treatment reduces their expressions.…”

Section: Discussionsupporting

confidence: 58%

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Song,

Niu,

Zhang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Purple sweet potato polysaccharide (PSPP-1) is a novel glucan; this study aimed to examine the anti-inflammatory effect of PSPP-1 and elucidate its potential mechanisms. Lipopolysaccharide (LPS)-induced RAW264.7 was used as the model of inflammation, cell viability, and levels of nitric oxide (NO), reactive oxygen species (ROS), and calcium ion (Ca 2+ ) were analyzed. ELISA and qPCR were used to assess the productions and mRNA expression of cytokines, and Western blotting was used to assess protein expressions in the TLR-mediated pathway, macrophage polarization, and inflammasome activation. The results demonstrated PSPP-1 inhibited cell proliferation and markedly decreased NO, ROS, and Ca 2+ levels. Moreover, PSPP-1 suppressed the secretions and mRNA expressions of pro-inflammatory cytokines and increased those of anti-inflammatory cytokines. Furthermore, PSPP-1 could exert anti-inflammatory effects through different pathways mediated by both TLR2 and TLR4, which modulated the expressions of essential proteins in the myeloid differentiation factor 88 (MyD88)-dependent and toll/IL-1 receptor domain-containing adaptor-inducing interferon-β (TRIF)-dependent signaling pathways. PSPP-1 even regulated the polarization of M1/M2 macrophages and inhibited the nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome activation. These findings indicate that PSPP-1 can suppress LPS-induced inflammation via multiple pathways and may be a potential agent for therapeutic inflammation-related pathophysiological processes and disorders.

show abstract

Section: Discussionsupporting

confidence: 58%

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Song,

Niu,

Zhang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In another study, TLR2 was found to be highly expressed in granulosa cells from PCOS patients and could mediate in ammation and oxidative stress caused by LPS 37 . These results showed that TLR2 was a key molecule mediating in ammation in COVID-19 and PCOS.…”

Section: Discussionmentioning

confidence: 94%

Discovering common pathogenetic processes between SARS-CoV-2 infection and polycystic ovary syndrome based on an integrated bioinformatics approach and experiment validation

Bai,

Zhang,

et al. 2024

Preprint

View full text Add to dashboard Cite

The prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among polycystic ovary syndrome (PCOS) is significantly higher than in the general population. However, the mechanisms underlying this remain obscure. This study aimed to explore the mechanisms by identifying genetic signature of SARS-CoV-2 infection in PCOS. In the present study, a total of 27 common differentially expressed genes (DEGs) were selected for subsequent analyses. Functional analyses showed that immunity and hormone related pathways collectively participated in the development and progression of PCOS and SARS Cov-2 infection. Under these, 7 significant hub genes were identified, including S100A9, MMP9, TLR2, THBD, ITGB2, ICAM1, CD86 by using the algorithm in Cytoscape. Furthermore, hub genes expression was confirmed in validation set, PCOS clinical samples and mouse model. Immune microenvironment analysis with CIBERSORTx database demonstrated that the hub genes were significantly correlated with T cells, dendritic cells, mast cells, B cells, NK cells, eosinophils and positively correlated with immune scores. Among the hub genes, S100A9, MMP9, THBD, ITGB2, CD86 and ICAM1 exhibited preferable values as diagnostic makers for COVID-19 and PCOS. In addition, we established the interaction networks of ovary-specific genes, transcription factors, miRNAs, drugs, and chemical compounds with hub genes with NetworkAnalyst. This work uncovered the common pathogenesis and genetic signature of PCOS and SARS-CoV-2 infection, which might provide a theoretical basis and innovative ideas for further mechanistic research and drug discovery of the comorbidity of two diseases.

show abstract

“…MMP9 has been confirmed to be associated with atherosclerotic thrombosis, endothelial dysfunction, and non-alcoholic fatty liver disease in PCOS patients (36)(37)(38). IL1B, IL6 and TNF are associated with PCOS inflammation, endoplasmic reticulum stress and recurrent abortion, and can be regulated by the active components of Leonuri Herba (39)(40)(41)(42)(43)(44). Leonuri Herba regulates the expression of these genes through AGE-RAGE, PI3K-Akt and MAPK signaling pathways.…”

Section: Discussionmentioning

confidence: 98%

The mechanism of Leonuri Herba in improving polycystic ovary syndrome was analyzed based on network pharmacology and molecular docking

Wu¹,

Liu²,

Zhang³

et al. 2023

J. pharm. pharm. sci.

View full text Add to dashboard Cite

Background: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder affecting women. Chinese herbs have been considered as an alternative treatment for PCOS, and Yi-mu-cao (Leonuri Herba) is one of the most commonly used herbs to treat PCOS, which can relieve symptoms of PCOS patients. But the mechanism of its treatment remains unclear.Method: The main active ingredients and potential targets of Leonuri Herba were obtained by TCMSP and Swiss Target Forecast, and the related targets of PCOS were obtained by searching DrugBank, GeneCard and DisGeNet databases. The Protein-Protein Interaction (PPI) network was constructed using STRING database. GO and KEGG were used to detect the enrichment pathways of key targets. Cytoscape software was used to construct the component-target-pathway network, analyze the PPI network core, and verify the reliability of target binding by molecular docking technology.Result: 8 components and 116 targets of Leonuri Herba on PCOS were screened. Common targets mainly involve the Lipid and atherosclerosis, Endocrine resistance, AGE-RAGE signaling in diabetic complications and other signaling pathways. It is suggested that it can form multi-target and multi-pathway regulatory network through quercetin, kaempferol and other active substances to regulate endocrine disorders and reduce inflammatory response, so as to systematically improve PCOS. Molecular docking experiments showed that the active constituents of Leonurus had good binding activity with potential targets of PCOS.Conclusion: In summary, this study elucidates the potential effect of Leonuri Herba on PCOS, which is helpful to provide reference for clinical practice. This is also conducive to the secondary development of motherwort and its monomer components, and precision medicine for PCOS.

show abstract

Resveratrol regulates the inflammation and oxidative stress of granulosa cells in PCOS via targeting TLR2

Cited by 13 publications

References 42 publications

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Discovering common pathogenetic processes between SARS-CoV-2 infection and polycystic ovary syndrome based on an integrated bioinformatics approach and experiment validation

The mechanism of Leonuri Herba in improving polycystic ovary syndrome was analyzed based on network pharmacology and molecular docking

Contact Info

Product

Resources

About