Retention and mass transfer properties of the series of unbonded, amide-bonded, and alkylsulfobetaine-bonded ethylene bridged hybrid hydrophilic interaction liquid chromatography columns

Gritti, Fabrice; Alden, Bonnie A.; McLaughlin, Justin; Walter, Thomas H.

doi:10.1016/j.chroma.2023.463828

Cited by 8 publications

(3 citation statements)

References 68 publications

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“…However, the stationary phase can influence the number of CRs. Specifically, the thickness of the water layer is linked to the nature of the stationary phase [50][51][52][53]. For example, bare silica stationary phases generate a thinner water layer than zwitterionic phases.…”

Section: Effect Of the Stationary Phasementioning

confidence: 99%

“…The impact of water within the pores is acknowledged not only in terms of compound retention but also in relation to equilibration duration [54] and column efficiency. This is attributed to differences in compound mass transfer based on the water layer thickness [52]. A comparison of pore size was performed using a BEH amide with 300 Å pore size.…”

Section: Effect Of the Stationary Phasementioning

confidence: 99%

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Impact of conditioning runs on hydrophilic interaction chromatography repeatability and its application as a second dimension in online comprehensive two‐dimensional liquid chromatography

Gély,

Monneau,

Hologne

et al. 2024

J of Separation Science

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A common separation approach for polar compounds involves coupling reversed‐phase liquid chromatography (RPLC) with hydrophilic interaction chromatography (HILIC) in two‐dimensional chromatography. The higher proportion of acetonitrile used in the HILIC mobile phase, which enhances mass spectrometry detection, encourages its use in the second dimension. Previous studies demonstrated that the HILIC column can be partially equilibrated within very short timeframes without compromising retention time stability, rendering it suitable in online comprehensive two‐dimensional liquid chromatography (LC×LC) setups. In addition, a specific number of conditioning cycles seems necessary to establish stable retention times. Here, the repeatability of HILIC when employed as second dimension in LC×LC was investigated, with a focus on determining the required number of conditioning cycles to achieve repeatable retention times. Various parameters influenced by the LC×LC online modulation system were studied, such as steep gradient slopes up to 8%, and very short equilibration times, less than or equal to dead time, as well as injection volume and solvent, which depend on the first dimension. Finally, the use of HILIC as a second dimension with tailored conditioning runs was applied to the analysis of hyaluronic acid hydrogel digests. The application of an RPLC×HILIC method using five conditioning runs yielded exceptional stability in second‐dimension retention times.

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Section: Effect Of the Stationary Phasementioning

confidence: 99%

Section: Effect Of the Stationary Phasementioning

confidence: 99%

Impact of conditioning runs on hydrophilic interaction chromatography repeatability and its application as a second dimension in online comprehensive two‐dimensional liquid chromatography

Gély,

Monneau,

Hologne

et al. 2024

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Hydrophilic interaction lilqid chromatography (HILIC) overcomes the disadvantages of both NP and RP chromatography in the separation of polar compounds due to its very different separation selectivity and good compatibility with electrospray ion source mass spectrometry [3]. According to Alpert [4], the retention mechanism of HILIC is primarily based on the partitioning of the solute between the mobile phase and the "water-rich layer", which is adsorbed by hydrophilic groups on the surface of the stationary phase Additionally, the interactions between the solutes, the stationary and the mobile phase also affect the separation performance for HILIC [5][6][7][8][9][10][11]. The selection of polar groups that create a 'waterrich layer' to modulate the interaction between the solute and the stationary phase resulting in improved separation selectivity is a crucial aspect of HILIC stationary phase research.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic interaction chromatographic evaluation of zwitterionic polymer grafted silica gel via multiple binding sites

Zhang,

Hu,

Liu

et al. 2024

J of Separation Science

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A novel zwitterionic polymer grafted silica stationary phase, Sil‐PZIC, was prepared by bonding poly(ethylene maleic anhydride) molecules on the surface of silica via multiple binding sites, followed by ammonolysis of maleic anhydride through a nucleophilic substitution reaction with ethylenediamine. The stationary phase was characterized by solid‐state 13C nuclear magnetic resonance, zeta potential, and elemental analysis and the results show the successful encapsulation of zwitterionic polymer on the surface of silica. The chromatographic performance of Sil‐PZIC was investigated by using nucleosides and nucleic bases as test analytes The variation of retention and separation performance of these model compounds were investigated by varying the chromatographic conditions such as the components of mobile phase, salt concentration, and pH. The results show that the retention of the Sil‐PZIC phase was dominated by a hydrophilic partitioning mechanism accompanied by secondary interactions such as electrostatic and hydrogen bonding. In addition, saccharides and Amadori compounds were also well separated on the Sil‐PZIC, indicating that the Sil‐PZIC column has potential application for separation of the polar compound.

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Managing sample introduction problems in hydrophilic interaction liquid chromatography

Taylor

Kawakami

McCalley

2023

Journal of Chromatography A

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Retention and mass transfer properties of the series of unbonded, amide-bonded, and alkylsulfobetaine-bonded ethylene bridged hybrid hydrophilic interaction liquid chromatography columns

Cited by 8 publications

References 68 publications

Impact of conditioning runs on hydrophilic interaction chromatography repeatability and its application as a second dimension in online comprehensive two‐dimensional liquid chromatography

Impact of conditioning runs on hydrophilic interaction chromatography repeatability and its application as a second dimension in online comprehensive two‐dimensional liquid chromatography

Hydrophilic interaction chromatographic evaluation of zwitterionic polymer grafted silica gel via multiple binding sites

Managing sample introduction problems in hydrophilic interaction liquid chromatography

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