Retention and selectivity in amino, cyano and diol normal bonded phase high-performance liquid chromatographic columns

Smith, P.; Cooper, William T.

doi:10.1016/s0021-9673(00)90058-x

Cited by 38 publications

(8 citation statements)

References 22 publications

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“…hexane : metyl-tert-butyl ether) than using unpolar solvents, although the difference of retention times is not outstanding due to the low retention capability of phenyl phases in general. 30 However, we found that when hexane is used as mobile phase at low pressures, nitro-PAHs are less retained than using polar solvents. On the other hand, we observed that when the pressure falls below 1 bar (Table 4) and under low speed flow (below 0.1 ml min À1 ), the order of elution of these classes of compounds was switched.…”

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confidence: 81%

Advances in PAHs/nitro-PAHs fractioning

2010

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The aim of this work was to develop an efficient methodology for the reliable fractioning of nitratedpolycyclic aromatic hydrocarbons (nitro-PAHs) and polycyclic aromatic hydrocarbons (PAHs). Unlike what usually occurs under pressures developed by HPLC (high performance liquid chromatography) systems (above 11 bar) we observed that when normal phase chromatographic fractioning procedures are accomplished under very low pressures (about 1 bar), dipole molecules (nitro-PAHs) elute much faster than non-polar organic molecules (PAHs). This finding allowed developing an original and very efficient methodology for fractioning nitro-PAHs and PAHs. This method is based on normal-phase liquid chromatography through a home-made phenyl column by using hexane as mobile phase at very low speed flow (0.05 ml min À1). Unlike typical HPLC methodology, the fractioning of nitro-PAHs and PAHs was accomplished as a function of their polarity (first the polar compounds as a unique peak and further, the non-polar compounds, PAHs) rather than as a function of their medium polarizability.

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confidence: 81%

Advances in PAHs/nitro-PAHs fractioning

2010

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“…It has been recently stated that the use of such stationary phases has been largely underestimated for liquid chromatography separations, 244 even though reports on properties and versatile applications of amino-, cyano- and diol-bonded Si-gel stationary phases were available for decades. 245–249…”

Section: Facing the Challenges In The Isolation Of Hydrophilic Compoundsmentioning

confidence: 99%

The isolation of water-soluble natural products – challenges, strategies and perspectives

et al. 2022

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“…The most commonly accepted retention mechanism is Snyder's model, which assumes the competitive adsorption between solutes and solvent molecules on active sites of the silica surface. Several studies have shown that this model is applicable for diol- [23,24], cyano- [23,25], and aminopropyl-bonded silica [26,27].…”

Section: Types Of Stationary Phasesmentioning

confidence: 99%